Improved Biolistic Transfection of Hair Cells

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Zhao, Hongyu ^a   Avenarius, Matthew R ^a   Gillespie, Peter G ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; HARMONIN B; HYBRID PROTEIN; PLASMA MEMBRANE CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE; PLASMA MEMBRANE CALCIUM TRANSPORTING ADENOSINE TRIPHOSPHATASE 2; PROTEIN ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BASILAR MEMBRANE; BULLFROG; CELL STRUCTURE; CHICK; COCHLEA; CONFOCAL MICROSCOPY; CONTROLLED STUDY; GENETIC TRANSFECTION; HAIR CELL; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; SACCULUS;

ACTINS; ANIMALS; BIOLISTICS; CHICKENS; FLUORESCENCE; HAIR CELLS, AMPULLA; MICE; MICROSCOPY, CONFOCAL; RANA CATESBEIANA; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

RANA CATESBEIANA;

EID: 84866979291     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0046765     Document Type: Article

References (39)

1. Hudspeth AJ, (1989) How the ear's works work. Nature 341: 397-404.
2. Yamoah EN, Lumpkin EA, Dumont RA, Smith PJ, Hudspeth AJ, et al. (1998) Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia. J Neurosci 18: 610-624.
3. Grillet N, Xiong W, Reynolds A, Kazmierczak P, Sato T, et al. (2009) Harmonin mutations cause mechanotransduction defects in cochlear hair cells. Neuron 62: 375-387.
4. Shin JB, Streijger F, Beynon A, Peters T, Gadzala L, et al. (2007) Hair bundles are specialized for ATP delivery via creatine kinase. Neuron 53: 371-386.
5. Wang H, Murphy R, Taaffe D, Yin S, Xia L, et al. (2011) Efficient cochlear gene transfection in guinea-pigs with adeno-associated viral vectors by partial digestion of round window membrane. Gene Ther 19: 255-263.
6. Karra D, Dahm R, (2010) Transfection techniques for neuronal cells. J Neurosci 30: 6171-6177.
7. Klimaschewski L, Nindl W, Pimpl M, Waltinger P, Pfaller K, (2002) Biolistic transfection and morphological analysis of cultured sympathetic neurons. J Neurosci Methods 113: 63-71.
8. Belyantseva IA, (2009) Helios Gene Gun-mediated transfection of the inner ear sensory epithelium. Methods Mol Biol 493: 103-123.
9. Hasson T, Gillespie PG, Garcia JA, MacDonald RB, Zhao Y, et al. (1997) Unconventional myosins in inner-ear sensory epithelia. J Cell Biol 137: 1287-1307.
10. Kachar B, Battaglia A, Fex J, (1997) Compartmentalized vesicular traffic around the hair cell cuticular plate. Hear Res 107: 102-112.
11. Ogris M, Steinlein P, Kursa M, Mechtler K, Kircheis R, et al. (1998) The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 5: 1425-1433.
12. Ma B, Zhang S, Jiang H, Zhao B, Lv H, (2007) Lipoplex morphologies and their influences on transfection efficiency in gene delivery. J Control Release 123: 184-194.
13. Ross PC, Hui SW, (1999) Lipoplex size is a major determinant of in vitro lipofection efficiency. Gene Ther 6: 651-659.
14. Zuidam NJ, Barenholz Y, (1999) Characterization of DNA-lipid complexes commonly used for gene delivery. Int J Pharm 183: 43-46.
15. Dalluge R, Haberland A, Zaitsev S, Schneider M, Zastrow H, et al. (2002) Characterization of structure and mechanism of transfection-active peptide-DNA complexes. Biochim Biophys Acta 1576: 45-52.
16. Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, et al. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 92: 7297-7301.
17. Finsinger D, Remy JS, Erbacher P, Koch C, Plank C, (2000) Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery. Gene Ther 7: 1183-1192.
18. Diebold SS, Cotten M, Koch N, Zenke M, (2001) MHC class II presentation of endogenously expressed antigens by transfected dendritic cells. Gene Ther 8: 487-493.
19. Zheng JL, Gao WQ, (2000) Overexpression of Math1 induces robust production of extra hair cells in postnatal rat inner ears. Nat Neurosci 3: 580-586.
20. Gubbels SP, Woessner DW, Mitchell JC, Ricci AJ, Brigande JV, (2008) Functional auditory hair cells produced in the mammalian cochlea by in utero gene transfer. Nature 455: 537-541.
21. Driver EC, Kelley MW (2010) Transfection of mouse cochlear explants by electroporation. Curr Protoc Neurosci Chapter 4: Unit 4.34.1-10.
22. Belyantseva IA, Boger ET, Friedman TB, (2003) Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle. Proc Natl Acad Sci USA 100: 13958-13963.
23. Thomas JL, Bardou J, L'hoste S, Mauchamp B, Chavancy G, (2001) A helium burst biolistic device adapted to penetrate fragile insect tissues. J Insect Sci 1: 9.
24. O'Brien JA, Holt M, Whiteside G, Lummis SC, Hastings MH, (2001) Modifications to the hand-held Gene Gun: improvements for in vitro biolistic transfection of organotypic neuronal tissue. J Neurosci Methods 112: 57-64.
25. Raju PA, McSloy N, Truong NK, Kendall MA, (2006) Assessment of epidermal cell viability by near infrared multi-photon microscopy following ballistic delivery of gold micro-particles. Vaccine 24: 4644-4647.
26. Zhang DS, Piazza V, Perrin BJ, Rzadzinska AK, Poczatek JC, et al. (2012) Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia. Nature 481: 520-524.
27. Reiners J, Reidel B, El-Amraoui A, Boeda B, Huber I, et al. (2003) Differential distribution of harmonin isoforms and their possible role in Usher-1 protein complexes in mammalian photoreceptor cells. Invest Ophthalmol Vis Sci 44: 5006-5015.
28. Bahloul A, Michel V, Hardelin JP, Nouaille S, Hoos S, et al. (2010) Cadherin-23, myosin VIIa and harmonin, encoded by Usher syndrome type I genes, form a ternary complex and interact with membrane phospholipids. Hum Mol Genet 19: 3557-3565.
29. Boeda B, El-Amraoui A, Bahloul A, Goodyear R, Daviet L, et al. (2002) Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle. EMBO J 21: 6689-6699.
30. Dumont RA, Lins U, Filoteo AG, Penniston JT, Kachar B, et al. (2001) Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles. J Neurosci 21: 5066-5078.
31. Hill JK, Williams DE, LeMasurier M, Dumont RA, Strehler EE, et al. (2006) Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles. J Neurosci 26: 6172-6180.
32. Holt JR, (2002) Viral-mediated gene transfer to study the molecular physiology of the Mammalian inner ear. Audiol Neurootol 7: 157-160.
33. Bedrosian JC, Gratton MA, Brigande JV, Tang W, Landau J, et al. (2006) In vivo delivery of recombinant viruses to the fetal murine cochlea: transduction characteristics and long-term effects on auditory function. Mol Ther 14: 328-335.
34. Rzadzinska AK, Schneider ME, Davies C, Riordan GP, Kachar B, (2004) An actin molecular treadmill and myosins maintain stereocilia functional architecture and self-renewal. J Cell Biol 164: 887-897.
35. Dazert S, Aletsee C, Brors D, Gravel C, Sendtner M, et al. (2001) In vivo adenoviral transduction of the neonatal rat cochlea and middle ear. Hear Res 151: 30-40.
36. Luebke AE, Foster PK, Muller CD, Peel AL, (2001) Cochlear function and transgene expression in the guinea pig cochlea, using adenovirus- and adeno-associated virus-directed gene transfer. Hum Gene Ther 12: 773-781.
37. Ishimoto S, Kawamoto K, Kanzaki S, Raphael Y, (2002) Gene transfer into supporting cells of the organ of Corti. Hear Res 173: 187-197.
38. Liu Y, Okada T, Sheykholeslami K, Shimazaki K, Nomoto T, et al. (2005) Specific and efficient transduction of cochlear inner hair cells with recombinant adeno-associated virus type 3 vector. Mol Ther 12: 725-733.
39. Siemens J, Kazmierczak P, Reynolds A, Sticker M, Littlewood-Evans A, et al. (2002) The Usher syndrome proteins cadherin 23 and harmonin form a complex by means of PDZ-domain interactions. Proc Natl Acad Sci U S A 99: 14946-14951.