Transfection techniques for neuronal cells

Journal of Neuroscience

Volumn 30, Issue 18, 2010, Pages 6171-6177

  Karra, Daniela ^a   Dahm, Ralf ^b,c  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY OF PADOVA   (Italy)

^c Centro Nacional de Investigaciones Oncológicas   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

LENTIVIRUS VECTOR;

ADENOVIRUS; BIOLISTIC TRANSFORMATION; ELECTROPORATION; GENE TARGETING; GENETIC TRANSFECTION; HERPES SIMPLEX VIRUS; HUMAN; MICROINJECTION; NERVE CELL; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; REVIEW;

ANIMALS; BIOLISTICS; ELECTROPORATION; HUMANS; NEURONS; TRANSDUCTION, GENETIC; TRANSFECTION;

EID: 77951809469     PISSN: 02706474     EISSN: 15292401     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.0183-10.2010     Document Type: Review

References (29)

1. Buning H, Perabo L, Coutelle O, Quadt-Humme S, Hallek M (2008) Recent developments in adeno-associated virus vector technology. J Gene Med 10:717-733. (Pubitemid 352003915)
2. Butcher AJ, Torrecilla I, Young KW, Kong KC, Mistry SC, Bottrill AR, Tobin AB (2009) N-methyl-D-aspartate receptors mediate the phosphorylation and desensitization of muscarinic receptors in cerebellar granule neurons. J Biol Chem 284:17147-17156.
3. Cockrell AS, Kafri T (2007) Gene delivery by lentivirus vectors. Mol Biotechnol 36:184-204.
4. Dahm R, Zeitelhofer M, Gotze B, Kiebler MA, Macchi P (2008) Visualizing mRNA localization and local protein translation in neurons. Methods Cell Biol 85:293-327.
5. Dalby B, Cates S, Harris A, Ohki EC, Tilkins ML, Price PJ, Ciccarone VC (2004) Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications. Methods 33:95-103. (Pubitemid 38569654)
6. Dib-Hajj SD, Choi JS, Macala LJ, Tyrrell L, Black JA, Cummins TR, Waxman SG (2009) Transfection of rat or mouse neurons by biolistics or electroporation. Nat Protoc 4:1118-1126.
7. Dieterlen MT, Wegner F, Schwarz SC, Milosevic J, Schneider B, Busch M, Romuss U, Brandt A, Storch A, Schwarz J (2009) Non-viral gene transfer by nucleofection allows stable gene expression in human neural progenitor cells. J Neurosci Methods 178:15-23.
8. Epstein AL (2009) HSV-1-derived amplicon vectors: recent technological improvements and remaining difficulties-a review. Mem Inst Oswaldo Cruz 104:399-410.
9. Fraefel C (2002) Gene delivery using helper virus-free HSV-1 amplicon vectors. Curr Protoc Hum Genet Chapter 12:Unit 12.12.
10. Goetze B, Grunewald B, Baldassa S, Kiebler M (2004) Chemically controlled formation of a DNA/calcium phosphate coprecipitate: application for transfection of mature hippocampal neurons. J Neurobiol 60:517-525. (Pubitemid 39108891)
11. Judkewitz B, Rizzi M, Kitamura K, Hausser M (2009) Targeted single-cell electroporation of mammalian neurons in vivo. Nat Protoc 4:862-869.
12. Kitamura K, Judkewitz B, Kano M, Denk W, Hausser M (2008) Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo. Nat Methods 5:61-67.
13. Knoll B, Kretz O, Fiedler C, Alberti S, Schutz G, Frotscher M, Nordheim A (2006) Serum response factor controls neuronal circuit assembly in the hippocampus. Nat Neurosci 9:195-204. (Pubitemid 43164542)
14. Liu M, Wang X, Geller AI (2009) Improved long-term expression from helper virus-free HSV-1 vectors packaged using combinations of mutated HSV-1 proteins that include the UL13 protein kinase and specific components of the VP16 transcriptional complex. BMC Mol Biol 10:58.
15. Lo DC, McAllister AK, Katz LC (1994) Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron 13:1263-1268.
16. Lowenstein PR, Mandel RJ, Xiong WD, Kroeger K, Castro MG (2007) Immune responses to adenovirus and adeno-associated vectors used for gene therapy of brain diseases: the role of immunological synapses in understanding the cell biology of neuroimmune interactions. Curr Gene Ther 7:347-360. (Pubitemid 47595777)
17. Murphy RC, Messer A (2001) Gene transfer methods for CNS organotypic cultures: a comparison of three nonviral methods. Mol Ther 3:113-121.
18. Rahim AA, Wong AM, Howe SJ, Buckley SM, Acosta-Saltos AD, Elston KE, Ward NJ, Philpott NJ, Cooper JD, Anderson PN, Waddington SN, Thrasher AJ (2009) Efficient gene delivery to the adult and fetal CNS using pseudotyped non-integrating lentiviral vectors. Gene Ther 16:509-520.
19. Royo NC, Vandenberghe LH, Ma JY, Hauspurg A, Yu L, Maronski M, Johnston J, Dichter MA, Wilson JM, Watson DJ (2008) Specific AAV serotypes stably transduce primary hippocampal and cortical cultures with high efficiency and low toxicity. Brain Res 1190:15-22.
20. Schratt GM, Tuebing F, Nigh EA, Kane CG, Sabatini ME, Kiebler M, Greenberg ME (2006) A brain-specific microRNA regulates dendritic spine development. Nature 439:283-289. (Pubitemid 43128846)
21. Simonato M, Manservigi R, Marconi P, Glorioso J (2000) Gene transfer into neurones for the molecular analysis of behaviour: focus on herpes simplex vectors. Trends Neurosci 23:183-190. (Pubitemid 30236175)
22. Tonges L, Lingor P, Egle R, Dietz GP, Fahr A, Bahr M (2006) Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons. RNA 12: 1431-1438. (Pubitemid 43990566)
23. Washbourne P, McAllister AK (2002) Techniques for gene transfer into neurons. Curr Opin Neurobiol 12:566-573.
24. White BH, Cummins TR, Wolf DH, Waxman SG, Russell DS, Kaczmarek LK (2002) HSV-1 helper virus 5dl1.2 suppresses sodium currents in amplicon-transduced neurons. J Neurophysiol 87:2149-2157.
25. Zeitelhofer M, Vessey JP, Xie Y, Tubing F, Thomas S, Kiebler M, Dahm R (2007) High-efficiency transfection of mammalian neurons via nucleofection. Nat Protoc 2:1692-1704.
26. Zeitelhofer M, Karra D, Vessey JP, Jaskic E, Macchi P, Thomas S, Riefler J, Kiebler M, Dahm R (2009a) High-efficiency transfection of short hairpin RNAs-encoding plasmids into primary hippocampal neurons. J Neurosci Res 87:289-300.
27. Zeitelhofer M, Vessey JP, Thomas S, Kiebler M, Dahm R (2009b) Transfection of cultured primary neurons via nucleofection. Curr Protoc Neurosci Chapter 4:Unit 4.32.
28. Zhang Y, Yu LC (2008) Single-cell microinjection technology in cell biology. Bioessays 30: 606-610. (Pubitemid 351859417)
29. Zhdanov RI, Kuvichkin VV, Shmyrina AS, Jdanov AR, Tverdislov VA (2002) Role of lipid membrane-nucleic acid interactions, DNA-membrane contacts and metal (II) cations in origination of initial cells and in evolution of prokaryotes to eukaryotes. Bioelectrochemistry 58:41-46.