First ring-trial validation of real-time PCR methods for the quantification of allergenic food ingredients

European Food Research and Technology

Volumn 235, Issue 4, 2012, Pages 619-630

  Siegel, Marion ^a   Schnur, Karin ^a   Boernsen, Britta ^a   Pietsch, Klaus ^a   Waiblinger, Hans Ulrich ^a  

^a Chemisches und Veterinäruntersuchungsamt Freiburg ^*  (Germany)

Author keywords

Allergen; Interlaboratory validation; Mustard; Quantification; Real time PCR; Soybean

Indexed keywords

ALLERGEN; INTERLABORATORY VALIDATION; MUSTARD; QUANTIFICATION; REAL-TIME PCR; SOYBEAN;

OILSEEDS;

POLYMERASE CHAIN REACTION;

GLYCINE MAX; SINAPIS ALBA;

EID: 84866537795     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-012-1788-6     Document Type: Article

References (27)

1. Directive 2000/13/EC. Official Journal of the European Union (OJ). L109 (06/05/2000) The European Parliament and the Council of the European Union, Brussels, p 29.
2. Regulation 2011/1169/EC. OJ L 304 (22/11/2011). The European Parliament and the Council of the European Union, Brussels, p 18.
3. State Institutes of Chemical and Veterinarian Analysis of Food in Baden-Wuerttemberg (CVUA) (2011) Allergens in food-situation remains unsatisfactory. http://www. cvuas. de/pub/beitrag. asp?subid=0& Thema_ID=9& ID=1429& Pdf=No. Cited 02. 07. 2012.
4. The Allergen Bureau. http://www. allergenbureau. net/. Cited 02. 07. 2012.
5. German Food Chemist Society, Working Group Biochemical and Molecularbiological Analysis: suppliers of allergen test kits. https://www. gdch. de/index. php?eID=tx_nawsecuredl& u=0& file=fileadmin/downloads/Netzwerk_und_Strukturen/Fachgruppen/Lebensmitte lchemiker/Arbeitsgruppen/analytik/test_kits_2009. pdf& t=1341477674& hash=37c61b0ef175740c45a20df7c77df1250344cc48 (Cited 02. 07. 2012).
6. Demmel A, Hupfer C, Ilg Hampe E, Busch U, Engel KH (2008) Development of a real-time PCR for the detection of lupine DNA (Lupinus Species) in foods. J Agric Food Chem 56(12): 4328-4332.
7. ® real-time polymerase chain reaction in comparison to commercially available protein-based enzyme-linked immunosorbent assay. Anal Chim Acta 24 685(1): 74-83.
8. Köppel R, Dvorak V, Zimmerli F, Breitenmoser A, Eugster A, Waiblinger HU (2010) Two tetraplex real-time PCR fort he detection and quantification of DNA from eight allergens in food. Eur Food Res Technol 230: 367-374.
9. Official Collection of Test Methods (OC) (2003) Determination of peanut contaminations in food with ELISA. German food and feed law-food analysis, Article 64, L 00. 00-69, Beuth-Verlag, Berlin.
10. OC (2006) Determination of hazelnut contaminations in chocolate and chocolate products with ELISA. German food and feed law-food analysis, Article 64, L 44. 00-7, Beuth-Verlag, Berlin.
11. OC (2008) Detection of a DNA sequence specific for celery (Apium graveolens) in boiled sausages with real-time PCR. German food and feed law-food analysis, Article 64, L 08. 00-56, Beuth-Verlag, Berlin.
12. OC (2010) Detection of a DNA sequence specific for hazelnut (Corylus avellana) in chocolate with real-time PCR. German food and feed law-food analysis, Article 64, L 44. 00-8, Beuth-Verlag, Berlin.
13. Fuchs M, Cichna-Markl M, Hochegger R (2010) J Agric Food Chem 2010(58): 11193-11200.
14. International Organization of Standardization (2005) ISO 21570: methods of analysis for the detection of genetically modified organisms and derived products-quantitative nucleic acid based methods. Annex C1.
15. L'Hocine L, Boye J, Munyaba C (2007) Detection and quantification of soy allergens in food: study of two commercial enzymed-linkend immunosorbent assays. J Food Sci 72(3): C145-C153.
16. Mustorp S, Engdahl-Axelsson C, Svensson U (2008) Detection of celery (apium graveolens), mustard (Sinapsis alba, Brassica juncea, Brassica nigra) and sesame (Sesamum indicum) in food by real-time PCR. Eur Food Res Technol 226: 771-778.
17. Kenk M, Panter S, Engler-Blum G, Bergemann J (2011) Sensitive DNA based allergen detection depends on food matrix and isolation method. Eur Food Res Technol. doi: 10. 1007/s00217-011-1639-x.
18. Demmel A, Hupfer C, Busch U, Engel KH (2011) Detection of lupine (Lupinus spp.) DNA in processed foods using real-time PCR. Food Control 22(2): 215-220.
19. International Union of Pure and Applied Chemistry, Analytical Chemistry Division (2006) The international harmonized protocol for the proficiency testing of analytical chemistry laboratories. Pure Appl Chem 78(1): 145-196.
20. OC (2012) Detection and quantification of mustard (Sinapis alba) and soybean (Glycine max) in boiled sausages with real-time PCR. German food and feed law-food analysis, Article 64, L 08. 00-59.
21. European Network of GMO Laboratories (ENGL) (2008) Definition of minimum performance requirements for analytical methods of GMO testing. http://gmo-crl. jrc. ec. europa. eu/doc/Min_Perf_Requirements_Analytical_methods. pdf (Cited 02. 07. 2012).
22. Waiblinger HU, Graf N, Broll H, Grohmann L, Pietsch K (2011) Evaluation of real-time PCR results at the limit of detection. J Verbr Lebensm. doi: 10. 1007/s00003-011-0669-4.
23. Horwitz W (1982) Evaluation of analytical methods for regulation of foods and drugs. Anal Chem 54: 67A-76A.
24. Dienstleistung Lebensmittel Analytik GbR (2007-2012) Proficiency test allergens evaluation reports. Großhansdorf, Germany.
25. ISO 5725-5 (1998) Accuracy (trueness and precision) of measurement methods and results-part 5: alternative methods for the determination of the precision of a standard measurement method.
26. Valoo 2. 4: Analytik-Software, Leer (Germany). http://www. analytik-software. de/ (Cited 02. 07. 2012).
27. Demmel A, Hupfer C, Busch U, Engel KH (2012) Quantification of lupine (Lupinus angustifolius) in wheat flour using realtime PCR and an internal standard material. Eur Food Res Technol. doi: 10. 1007/s00217-012-1741-8.