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The sulfated carbohydrate-protein linkage region isolated from chondroitin 4-sulfate chains of inter-α-trypsin inhibitor in human plasma
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Yamada S, Oyama M, Kinugasa H, Nakagawa T, Kawasaki T, Nakasawa S, Khoo KH, Dell A, Sugahara K: The sulfated carbohydrate-protein linkage region isolated from chondroitin 4-sulfate chains of inter-α-trypsin inhibitor in human plasma. Glycobiology 1995, 5:335-341.
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2-Glc) O-glycosidically linked to a serine residue in the first epidermal growth factor-like domain of human factors VII and IX and protein Z and bovine protein Z
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Lo-Guidice JM, Wieruszeski JM, Lemoine J, Verbert A, Roussel P, Lamblin G: Sialylatlon and sulfation of the carbohydrate chains in respiratory muclns from a patient with cystic fibrosis. J Biol Chem 1994, 269:18794-18813.
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Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzies latipes
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Taguchi T, Seko A. Kitajima K, Muto Y, Inoue S, Khoo KH, Morris HR, Dell A, Inoue Y: Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzies latipes. J Biol Chem 1994, 269:8762-8771. In this paper, extensive use is made of chemical degradation/derivatization methods, as well as LSI mass spectrometry using a 20-25 kV primary cesium ion beam and NMR techniques, to characterize the N-glycan structures in L-hyosophorin isolated from the fertilized eggs of Oryzias latipes and Oryzias melastigma. This study represents the first detailed investigation establishing the highly branched nature of these N-linked pentantennary glycans. Expression of such bulky multiantennary structures in hyosophorin molecules suggests that they may play a rob in regulating cell-cell interactions of blastomeres at certain developing stages of embryos.
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Inoue, Y.9
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A precise structural analysis of a fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of euryhaline killl fish (Fundulus heteroclitus). Novel penta-antennary N-glycan chains with a bisecting N-acetylglucosaminyl residue
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Taguchi T, Kitajima K, Muto Y, Inoue S, Khoo KH, Morris HR, Dell A, Wallace RA, Selman K, Inoue Y: A precise structural analysis of a fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of euryhaline killl fish (Fundulus heteroclitus). Novel penta-antennary N-glycan chains with a bisecting N-acetylglucosaminyl residue. Glycobiology 1995, 5:611-624.
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Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoletin secreted by recombinant BHK-21 cells
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Nimtz M, Wray V, Rüdiger A, Conradt HS: Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoletin secreted by recombinant BHK-21 cells. FEBS Lett 1995, 365:203-208.
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Primary structure of the O-glycosidically linked glycan chain of the crystalline surface layer glycoprotein of Thermoanaerobacter thermohydrosuffuricus L111-69
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Bock K, Schuster-Kolbe J, Altman E, Allmaier G, Stahl B, Christian R, Sleytr UB, Messner P: Primary structure of the O-glycosidically linked glycan chain of the crystalline surface layer glycoprotein of Thermoanaerobacter thermohydrosuffuricus L111-69. J Biol Chem 1994, 269:7137-7144.
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Hemling ME, Mentzer MA, Capiau C, Carr SA: A multifaceted strategy for the characterization of recombinant gD-2, a potential herpes vaccine. In Mass Spectrometry in the Biological Sciences. Edited by Burlingame AL, Carr SA. Totowa, New Jersey: Humana Press; 1996:307-331. This chapter illustrates the effective use of all of the newest techniques of mass spectrometry in the characterization of the glycosylation of the potential herpes vaccine recombinant gD-2, expressed in Chinese hamster ovary cells. It also includes a discussion of the tolerance and intolerance of these techniques to common buffers, salts, and detergents used in biochemical laboratories.
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Carbohydrate structures of β-trace protein from human cerebrospinal fluid: Evidence for 'brain-type' N-glycosylation
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Hoffmann A, Nimtz M, Wurster U, Conradt HS: Carbohydrate structures of β-trace protein from human cerebrospinal fluid: evidence for 'brain-type' N-glycosylation. J Neurochem 1994, 63:2185-2196.
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Novel tyvelose-containlng tri- And tetra-antennary N-glycans in the immunodomlnant antigens of the intracellular parasite Trichinella spiralis
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Reason AJ, Ellis LA, Appleton JA, Wisnewski N, Grieve RB, McNeil M, Wassom DL, Morris HR, Dell A: Novel tyvelose-containlng tri- and tetra-antennary N-glycans in the immunodomlnant antigens of the intracellular parasite Trichinella spiralis. Glycobiology 1994, 4:593-603.
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A unique multifucosylated - 3GalNAcβ1→4GlcNAcβ1→3Galα1 - motif constitutes the repeating unit of the complex O-glycans derived from the cercarial glycocalyx of Schistosoma mansoni
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Khoo KH, Sarda S, Xu X, Cauffield JP, McNeil MR, Homans SW, Morris HR, Dell A: A unique multifucosylated - 3GalNAcβ1→4GlcNAcβ1→3Galα1 - motif constitutes the repeating unit of the complex O-glycans derived from the cercarial glycocalyx of Schistosoma mansoni. J Biol Chem 1995, 270:17114-17123. This paper is an excellent illustration of how the well established techniques of selective degradation and derivatization, coupled with mass spectrometry, can be used to reveal and define unanticipated structures in protein glycosylation.
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Khoo, K.H.1
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Glycosylation of two recombinant human uterine tissue plasminogen activator variants carrying an additional N-glycosylation site in the epidermal-growth-factor-like domain
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Pfeiffer G, Strube KH, Schmidt M, Geyer R: Glycosylation of two recombinant human uterine tissue plasminogen activator variants carrying an additional N-glycosylation site in the epidermal-growth-factor-like domain. Eur J Biochem 1994, 219:331-346.
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Mass spectrometric studies on the N-linked oligosaccharides of baculovirus-expressed mouse interleukin-3
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Studies on the order and site specificity of GaINAc transfer to MUC1 tandem repeats by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase from milk or mammary carcinoma cells
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Stadie TRE, Chai W, Lawson AM, Byfield PGH, Hanisch FG: Studies on the order and site specificity of GaINAc transfer to MUC1 tandem repeats by UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase from milk or mammary carcinoma cells. Eur J Biochem 1995, 229:140-147.
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Eur J Biochem
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Comparative mapping of recombinant proteins and glycoproteins by plasma desorption and matrix-assisted laser desorption/ionization mass spectrometry
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Tsarbopoulos A, Karas M, Strupat K, Pramanik BN, Nagabhushan TL, Hillenkamp F: Comparative mapping of recombinant proteins and glycoproteins by plasma desorption and matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem 1994, 66:2062-2070. Deposition of excess internal energy in analyte molecular ions during the MALDI process causes metastable fragmentation, the extent of which is matrix dependent. In practical terms, the analysis of glycopeptides may be compromised by the lability (partial loss) of sialic acid residues as well as other small neutral species. These authors show that this problem may be ameliorated to some extent using a 9:1 mixture of 2,5-dihydroxybenzoic acid (DHB) and 2-hydroxy-5-methoxybenzoic acid instead of neat DHB.
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Anal Chem
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Karas, M.1
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Tandem mass spectrometry and structural elucidation of glycopeptides from a hydroxyproline-rich plant cell wall glycoprotein indicate that contiguous hydroxyproline residues are the major sites of hydroxyproline O-arabinosylation
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Kieliszewski MJ, O'Neill M, Leykam J, Orlando R: Tandem mass spectrometry and structural elucidation of glycopeptides from a hydroxyproline-rich plant cell wall glycoprotein indicate that contiguous hydroxyproline residues are the major sites of hydroxyproline O-arabinosylation. J Biol Chem 1995, 270:2541-2549. Tandem mass spectrometry on an underivatized O-linked plant cell wall glycopeptide is used to determine which hydroxyproline residue is glycosylated. Precise hydroxyproline-arabinosylation motifs were established, suggesting a sequence-dependent, rather than conformation-dependent, enzymatic mechanism.
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Laser desorption time-of-flight mass spectrometric analysis of transferrin precipitated with antiserum: A unique simple method to identify molecular weight variants
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Carbohydrate structure analysis of batroxobin, a thrombin-like serine protease from Bothrops moojen/ venom
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Purification and amino acid sequence of aminopeptidase P from pig kidney
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Vergas Romero C, Neudorfer I, Mann K, Schäfer W: Purification and amino acid sequence of aminopeptidase P from pig kidney. Eur J Biochem 1995, 229:262-269.
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Eur J Biochem
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Matrix-assisted laser desorption/ionization mass spectrometry: Improved matrix for oligosaccharides
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Characterization and direct glycoform profiling of a hybrid plasminogen activator by matrix-assisted laser desorption and electrospray mass spectrometry: Correlation with high-performance liquid chromatographic and nuclear magnetic resonance analyses of the released glycans
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Müller D, Domon B, Karas M, Van Oostrum J, Richter WJ: Characterization and direct glycoform profiling of a hybrid plasminogen activator by matrix-assisted laser desorption and electrospray mass spectrometry: correlation with high-performance liquid chromatographic and nuclear magnetic resonance analyses of the released glycans. Biol Mass Spectrom 1994, 23:330-338.
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Mass spectrometry of the humanized monoclonal antibody CAMPATH 1H
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Analysis of the N-linked oligosaccharides of human C1s using electrospray Ionisation mass spectrometry
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Petillot Y, Thibault P, Thielens NM, Rossi V, Lacroix M, Coddeville B, Spik G, Schumaker VN, Gagnon J, Arlaud GJ: Analysis of the N-linked oligosaccharides of human C1s using electrospray Ionisation mass spectrometry. FEBS Lett 1995, 358:323-358.
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C-terminal post-translational proteolysis of plant lectins and their recombinant forms expressed in Escherichia coli
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Characterization of humanized anti-TAC, an antibody directed against the interleukin 2 receptor, using electrospray ionization mass spectrometry by direct infusion, LC/MS, and MS/MS
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Lewis DA, Guzzetta AW, Hancock WS, Costello M: Characterization of humanized anti-TAC, an antibody directed against the interleukin 2 receptor, using electrospray ionization mass spectrometry by direct infusion, LC/MS, and MS/MS. Anal Chem 1994, 66:585-595.
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The oligosaccharides of the Fe(III)-Zn(II) purple acid phosphatase of the red kidney bean
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Stahl B, Klabunde T, Witzel H, Krebs B, Steup M, Karas M, Hillenkamp F: The oligosaccharides of the Fe(III)-Zn(II) purple acid phosphatase of the red kidney bean. Eur J Biochem 1994, 220:321-330.
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