Specific labeling of threonine methyl groups for NMR studies of protein-nucleic acid complexes

Biochemistry

Volumn 50, Issue 47, 2011, Pages 10189-10191

  Sinha, Kaustubh ^a   Jen Jacobson, Linda ^a   Rule, Gordon S ^b  

^a UNIVERSITY OF PITTSBURGH   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID-BINDING PROTEINS; BIOSYNTHETIC PATHWAY; DYNAMIC STUDIES; GROWTH MEDIUM; LABELING SCHEME; METHYL GROUP; NMR STUDIES; PROTEIN-NUCLEIC ACIDS; RNA INTERFACE;

AMINO ACIDS; DEUTERIUM; ESCHERICHIA COLI; FUNCTIONAL GROUPS; GLYCEROL; RNA;

PROTEINS;

ALANINE; CARBON 13; DNA; GLYCEROL; ISOLEUCINE; LYSINE; METHIONINE; METHYL GROUP; NUCLEIC ACID; RNA; THREONINE; VALERIC ACID DERIVATIVE;

ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; CULTURE MEDIUM; ESCHERICHIA COLI; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DNA INTERACTION; PROTEIN RNA BINDING; PROTEIN STRUCTURE;

DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; RNA, BACTERIAL; STAINING AND LABELING; THREONINE;

ESCHERICHIA COLI;

EID: 81855190757     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201496d     Document Type: Article

References (23)

1. Boehr, D. D., Dyson, H. J., and Wright, P. E. (2006) Chem. Rev. 106, 3055-3079
2. Kay, L. E. (2005) J. Magn. Reson. 173, 193-207
3. Tzakos, A. G., Grace, C. R., Lukavsky, P. J., and Riek, R. (2006) Annu. Rev. Biophys. Biomol. Struct. 35, 319-342
4. Tugarinov, V., Hwang, P. M., Ollerenshaw, J. E., and Kay, L. E. (2003) J. Am. Chem. Soc. 125, 10420-10428
5. Rosen, M. K., Gardner, K. H., Willis, R. C., Parris, W. E., Pawson, T., and Kay, L. E. (1996) J. Mol. Biol. 263, 627-636
6. Tugarinov, V., Kanelis, V., and Kay, L. E. (2006) Nat. Protoc. 1, 749-754
7. Ruschak, A. M., Velyvis, A., and Kay, L. E. (2010) J. Biomol. NMR 48, 129-135
8. Tugarinov, V. and Kay, L. E. (2004) J. Am. Chem. Soc. 126, 9827-9836
9. Korzhnev, D. M., Kloiber, K., Kanelis, V., Tugarinov, V., and Kay, L. E. (2004) J. Am. Chem. Soc. 126, 3964-3973
10. Tugarinov, V., Ollerenshaw, J. E., and Kay, L. E. (2005) J. Am. Chem. Soc. 127, 8214-8225
11. Ishima, R., Louis, J. M., and Torchia, D. A. (1999) J. Am. Chem. Soc. 121, 11589-11590
12. Ayala, I., Sounier, R., Use, N., Gans, P., and Boisbouvier, J. (2009) J. Biomol. NMR 43, 111-119
13. Lundstrom, P., Teilum, K., Carstensen, T., Bezsonova, I., Wiesner, S., Hansen, D. F., Religa, T. L., Akke, M., and Kay, L. E. (2007) J. Biomol. NMR 38, 199-212
14. Higman, V. A., Flinders, J., Hiller, M., Jehle, S., Markovic, S., Fiedler, S., van Rossum, B. J., and Oschkinat, H. (2009) J. Biomol. NMR 44, 245-260
15. Lo Conte, L., Chothia, C., and Janin, J. (1999) J. Mol. Biol. 285, 2177-2198
16. Bahadur, R. P., Zacharias, M., and Janin, J. (2008) Nucleic Acids Res. 36, 2705-2716
17. Biswas, S., Guharoy, M., and Chakrabarti, P. (2009) Proteins 74, 643-654
18. Studier, F. W. and Moffatt, B. A. (1986) J. Mol. Biol. 189, 113-130
19. Otten, R., Chu, B., Krewulak, K. D., Vogel, H. J., and Mulder, F. A. (2010) J. Am. Chem. Soc. 132, 2952-2960
20. Briercheck, D. M., Wood, T. C., Allison, T. J., Richardson, J. P., and Rule, G. S. (1998) Nat. Struct. Biol. 5, 393-399
21. LeMaster, D. M. and Kushlan, D. M. (1996) J. Am. Chem. Soc. 118, 9255-9264
22. McCallum, S. A., Hitchens, T. K., and Rule, G. S. (1999) J. Mol. Biol. 285, 2119-2132
23. Takeuchi, K., Ng, E., Malia, T. J., and Wagner, G. (2007) J. Biomol. NMR 38, 89-98