Changing the specificity of α-amino acid ester hydrolase toward para-hydroxyl cephalosporins synthesis by site-directed saturation mutagenesis

Biotechnology Letters

Volumn 34, Issue 9, 2012, Pages 1719-1724

  Ye, Li Juan ^a,b   Wang, Lu ^a,b   Pan, Yue ^b   Cao, Yi ^a  

^a SICHUAN UNIVERSITY   (China)

^b Sichuan Industrial Institute of Antibiotics   (China)

Author keywords

Amino acid ester hydrolase; Cefprozil; Model product screening; Site directed saturation mutagenesis; Substrate specificity

Indexed keywords

96-WELL FORMAT; AMINO ACID ESTERS; AMINO GROUP; CEFADROXIL; CEFPROZIL; INITIAL RATE; SATURATION MUTAGENESIS; SPECTROPHOTOMETRIC ASSAYS; SUBSTRATE RECOGNITION; SUBSTRATE SPECIFICITY; XANTHOMONAS;

AMINO ACIDS; CLONING; ESCHERICHIA COLI; ESTERIFICATION; ESTERS; GENES; HYDROLASES; MUTAGENESIS;

ANTIBIOTICS;

ALPHA AMINO ACID ESTERASE; ALPHA-AMINO ACID ESTERASE; CARBOXYLESTERASE; CEPHALOSPORIN DERIVATIVE;

ARTICLE; BIOSYNTHESIS; CHEMICAL STRUCTURE; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; XANTHOMONAS;

CARBOXYLIC ESTER HYDROLASES; CEPHALOSPORINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; XANTHOMONAS;

ESCHERICHIA COLI; XANTHOMONAS;

EID: 84865785490     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-0955-y     Document Type: Article

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