High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli

Biotechnology and Bioengineering

Volumn 109, Issue 10, 2012, Pages 2533-2542

  Matos, Cristina F R O ^a   Branston, Steven D ^b   Albiniak, Anna ^a   Dhanoya, Arjun ^b   Freedman, Robert B ^a   Keshavarz Moore, Eli ^b   Robinson, Colin ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Cell engineering; E. coli; Fermentation; Protein export; Tat pathway

Indexed keywords

BATCH FERMENTATION; DOWNSTREAM-PROCESSING; E. COLI; EXPORT VALUE; FED-BATCH FERMENTATION; FEED-RATES; FERMENTATION PROCESS; FOLDED STATE; GREEN FLUORESCENT PROTEIN; HETEROLOGOUS PROTEINS; IN-LINE; INDUCTION PERIODS; INDUSTRIAL PRODUCTION; PERIPLASM; SIGNAL PEPTIDE; SUBSTRATE PROTEINS; TAT PATHWAY; TAT SIGNAL PEPTIDE; TRANSLOCATION PATHWAY; TWIN-ARGININE TRANSLOCATIONS; WILD-TYPE CELLS;

AMINO ACIDS; CELL CULTURE; CELL ENGINEERING; CELL MEMBRANES; ESCHERICHIA COLI; GENE ENCODING; GLYCEROL; PURIFICATION; RECOMBINANT PROTEINS;

FERMENTATION;

GLYCEROL; GREEN FLUORESCENT PROTEIN; TRANSACTIVATOR PROTEIN; TWIN ARGININE TRANSLOCATION SYSTEM; UNCLASSIFIED DRUG;

ARTICLE; CYTOPLASM; ESCHERICHIA COLI; FED BATCH FERMENTATION; NONHUMAN; PROTEIN DEGRADATION; WILD TYPE;

BIOTECHNOLOGY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; MEMBRANE TRANSPORT PROTEINS; METABOLIC ENGINEERING; PERIPLASM; PROTEIN TRANSPORT; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 84865376795     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24535     Document Type: Article

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