Production of antibody fragments in Escherichia coli

Annals of the New York Academy of Sciences

Volumn 782, Issue , 1996, Pages 143-158

  Harrison, J S ^a   Keshavarz Moore, E ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT PROTEIN;

ANTIBODY PRODUCTION; ANTIBODY STRUCTURE; ANTIBODY TITER; CELL INCLUSION; CELL SURFACE; CONFERENCE PAPER; CULTURE MEDIUM; CYTOPLASM; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY;

ESCHERICHIA COLI;

EID: 0029945829     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb40556.x     Document Type: Conference Paper

References (81)

1. STRANDBERG, L., K. KOHLER & S.-O. ENFORS. 1991. Large-scale fermentation and purification of a recombinant protein from Escherichia coli. Proc. Biochem. 26: 225-234.
2. DATAR, R. V., T. CARTWRIGHT & C.-G. ROSEN. 1993. Process economics of animal cell and baterial fermentations: A case study analysis of tissue plasminogen activator. Bio/Technology 11: 349-357.
3. SANDHU, J. S. 1992. Protein engineering of antibodies. Crit. Rev. Biotechnol. 12: 437-462.
4. JONES, P. T. 1992. Antibody engineering. Biochem. Soc. Trans. 20: 203-207.
5. BIRD, R. E. & B. W. WALKER. 1991. Single chain antibody variable regions. Trends Biotechnol. 9: 132-137.
6. PLUCKTHUN, A. & A: SKERRA. 1989. Expression of functional antibody Fv and Fab fragments in Escherichia coli. Methods Enzymol. 178: 497-515.
7. BETTER, M. & A. H. HORWITZ. 1989. Expression of engineered antibodies and antibody fragments in microorganisms. Methods Enzymol. 178: 476-496.
8. PLUCKTHUN, A. 1992. Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding. Immunol. Rev. 130: 151-188.
9. KING, D. J., O. D. BYRON, A. MOUNTAIN, N. WEIR, A. HARVEY, A. D. G. LAWSON, K. A. PROUDFOOT, D. BALDOCK, S. E. HARDING, G. T. YARRANTON & R. J. OWENS. 1993. Expression, purification and characterisation of B72.3 Fv fragments. Biochem. J. 290: 723-729.
10. CARTER, P., R. F. KELLEY, M. L. RODRIGUES, B. SNEDCOR, M. COVARRUBIAS, M. D. VELLIGAN, W. L. T. WONG, A. M. ROWLAND, C. E. KOTTS, M. E. CARVER, M. YANG, J. H. BOURELL, H. M. SHEPARD & D. HENNER. 1992. High level Escherichia coli expression and production of a bivalent humanizes antibody fragment. Bio/Technology 10: 163-167.
11. PORTER, R. R. 1959. The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain. Biochem. J. 73: 119-126.
12. INBAR, D., J. HOCHMAN & D. GIVOL. 1972. Localization of antibody-combining sites within the variable portions of heavy and light chains. PNAS 69: 2659-2662.
13. HOCHMAN, J., D. INBAR & D. GIVOL. 1973. An active antibody fragment (Fv) composed of the variable portions of heavy and light chains. Biochemistry 12: 1130-1135.
14. BIRD, R. E., K. D. HARDMAN, J. W. JACOBSON, S. JOHNSON, B. M. KAUFMAN, S.-M. LEE, T. LEE, S. H. POPE, G. S. RIORDAN & M. WHITLOW. 1988. Single chain antigen binding proteins. Science 242: 423-426.
15. HUSTON, J. S., D. LEVINSON, M. MUDGETT-HUNTER, M.-S. TAI, J. NOVOTNY, M. N. MARGOLIES, R. J. RIDGE, R. E. BRUCCOLERI, E. HABER, R. CREA & H. OPPERMANN. 1988. Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single chain Fv analogue produced in Escherichia coli. PNAS 85: 5879-5883.
16. PANTOLIANO, M. W., R. E. BIRD, S. JOHNSON, E. D. ASEL, S. W. DODD, J. F. WOOD & K. D. HARDMAN. 1991. Conformational stability and ligand-binding affinity of single-chain Fv immunoglobulin fragments in Escherichia coli. Biochemistry 30: 10117-10125.
17. COLCHER, D., R. BIRD, M. ROSELLI, K. D. HARDMAN, S. JOHNSON, S. POPE, S. W. DODD, M. W. PANTOLIANO, D. E. MILENIC & J. SCHLOM. 1990. In vivo tumor targeting of a recombinant single-chain antigen-binding protein. J. Natl. Cancer Inst. 82: 1191-1197.
18. BERRY, M. J. & J. J. PIERCE. 1993. Stability of immunoadsorbents comprising antibody fragments comparison of Fv fragments and single chain Fv fragments. J. Chromatog. 629: 161.
19. TAI, M.-S., M. MUDGETT-HUNTER, D. LEVINSON, G.-M. WU, E. HABER, H. OPPERMANN & J. S. HUSTON. 1990. A bifunctional fusion protein containing Fc binding fragment B of staphylococcal protein A amino terminal to antidigoxin single-chain Fv. Biochemistry 29: 8024-8030.
20. NEUBERGER, M. S., G. T. WILLIAMS & R. O. Fox. 1984. Recombinant antibodies possessing novel effector functions. Nature 312: 604-608.
21. SKERRA, A., I. PFITZINGER & A. PLUCKTHUN. 1991. The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique. Bio/Technology 9: 273-278.
22. PACK, P., M. KUJAU, V. SCHROECKH, U. KNUPFER, R. WENDEROTH, D. REISENBERG & A. PLUCKTHUN. 1993. Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli. Bio/ Technology 11: 1271-1277.
23. LAUKKANEN, M.-L., T. T. TEERI & K. KEINANEN. 1993. Lipid-tagged antibodies: Bacterial expression and characterization of a lipoprotein-single-chain antibody fusion protein. Protein Eng. 6: 449-454.
24. FIELD, H. 1990. Recombinant immunoglobulin variable domain production - feasibility, progress and directions. Chimicaoggi Sept.: 33-36.
25. PLUCKTHUN, A. 1991. Antibody engineering: Advances from the use of Escherichia coli expression systems. Bio/Technology 9: 545-551.
26. BOSS, M. A., J. H. KENTEN, C. R. WOOD & J. S. EMTAGE. 1984. Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli. Nucleic Acids Res. 12: 3791-3806.
27. CABILLY, S., A. D. RIGGS, H. PANDE, J. E. SHIVELY, W. E. HOLMES, M. REY, L. J. PERRY, R. WETZEL & H. L. HEYNEKER. 1984. Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli. PNAS 81: 3273-3277.
28. FIELD, H., G. T. YARRANTON & A. R. REES. 1989. Expression of mouse immunoglobulin light and heavy chain variable regions in Escherichia coli and reconstitution of antigen-binding activity. Protein Eng. 3: 641-647.
29. CHEADLE, C., L. E. HOOK, D. GIVOL & G. A. RICCA. 1992. Cloning and expression of the variable regions of mouse myeloma protein MOPC315 in E. coli: Recovery of active Fv fragments. Mol. Immunol. 29: 21-30.
30. KENTEN, J., B. HELM, T. ISHIZAKA, P. CATTINI & H. GOULD. 1984. Properties of a human immunoglobulin ε-chain fragment synthesized in Escherichia coli. PNAS 81: 2955-2959.
31. BUCHNER, J. & R. RUDOLPH. 1991. Renaturation, purification and characterisation of recombinant Fab fragments produced in Escherichia coli. Bio/Technology 9: 157-164.
32. SKERRA, A. & A. PLUCKTHUN. 1988. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 240: 1038-1041.
33. HSIUNG, H. M., N. G. MAYNE & G. W. BECKER. 1986. High-level expression, secretion and folding of human growth hormone in Escherichia coli. Bio/Technology 4: 991-995.
34. GLOCKSHUBER, R., M. MALIA, I. PFITZINGER & A. PLUCKTHUN. 1990. A comparison of strategies to stabilize immunoglobulin Fv fragments. Biochemistry 29: 1362-1367.
35. PLUCKTHUN, A. & I. PFITZINGER. 1991. Comparison of the Fv fragments of different phosphorylcholine binding antibodies expressed in Escherichia coli. Ann. N. Y. Acad. Sci. 646: 115-124.
36. BREGEGERE, F. & H. BEDOUELLE. 1992. Expression, export and purification of antibody fragments fused to the maltose-binding protein of Escherichia coli. C. R. Seances Acad. Sci. Serie 3 314: 527-532.
37. BETTER, M., C. P. CHANG, R. R. ROBINSON & A. H. HORWITZ. 1988. Escherichia coli secretion of an active chimeric antibody fragment. Science 240: 1041-1043.
38. FRANCISCO, J. A., R. CAMPBELL, B. L. IVERSON & G. GEOGIOU. 1993. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. PNAS 90: 10444-10448.
39. FUCHS, P., F. BREITLING, S. DUBEL, T. SEEHAUS & M. LITTLE. 1991. Targeting recombinant antibodies to the surface of Escherichia coli: Fusion to a peptidoglycan associated lipoprotein. Bio/Technology 9: 1369-1372.
40. BANYEX, F., A. AYLING, T. PALUMBO, D. THOMAS & G. GEORGIOU. 1991. Optimisation of growth conditions for the production of proteolytically sensitive proteins in the periplasmic space of Escherichia coli. Appl. Microbiol. Biotechnol. 36: 14-20.
41. WULFING, C. & A. PLUCKTHUN. 1994. Protein folding in the periplasm of Escherichia coli. Mol. Microbiol. 12: 685-692.
42. KNAPPIK, A., C. KREBBER & A. PLUCKTHUN. 1993. The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli. Bio/Technology 11: 77-83.
43. SUOMINEN, I., M. KARP, M. LAHDE, A. KOPIO, T. GLUMOFF, P. MEYER & P. MANTSALA. 1987. Extracellular Production of cloned α-amylase by Escherichia coli. Gene 161: 165-176.
44. SEO, J. H., A. I. LOFFLER & J. E. BAILEY. 1988. A parametric study of cloned fusion protein expression in Escherichia coli. Biotechnol. Bioeng. 32: 725-730.
45. WARD, E. S., D. GUSSOW, A. D. GRIFFITHS, P. T. JONES & G. WINTER. 1989. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341: 544-546.
46. TAKKINEN, K., M.-L. LAUKKANEN, D. SIZMANN, K. ALFTHAN, T. IMMONEN, L. VANNE, M. KAARTINEN, J. K. C. KNOWLES & T. T. TEERI. 1991. An active single-chain antibody containing a cellulase linker domain is secreted by Escherichia coli. Protein Eng. 4: 837-841.
47. AYALA, M., M. DUENAS, A. SANTOS, J. VAZQUEZ, A. MENDEZ, A. SILVA & J. V. GAVILONDO. 1992. Bacterial single-chain antibody fragments, specific for carcinoembryonic antigen. BioTechniques 13: 790-799.
48. BETTER, M. & A. H. HORWITZ. 1993. In vivo expression of correctly folded antibody fragments from microorganisms. ACS Symp. Ser. 526: 203-217.
49. CHESTER, K. A., L. ROBSON, P. A. KEEP, R. B. PEDLEY, J. A. BODEN, R. E. HAWKINS & R. H. J. BEGENT. 1994. Production and Tumour-Binding Characterization of a Chimeric Anti-CEA Fab Expressed in Escherichia coli. Intl. J. Cancer 57: 67-72.
50. DUENAS, M., J. VAZQUEZ, M. AYALA, E. SODERLIND, M. OHLIN, L. PEREZ, C. A. K. BORREBAECK & J. V. GAVILONDO. 1994. Intra- and extracellular expression of an scFvantibody fragment in E. coli: Effect of bacterial strains and pathway engineering using GroESLchaperonins. BioTechniques 16: 476-483.
51. BERRY, M. J., T. A. K. WATTAM, J. WILLETS, N. LINDNER, T. DE GRAAF, T. HUNT, M. GANI, P. J. DAVIS & P. PORTER. 1994. Assay and purification of Fv fragments in fermenter cultures: Design and evaluation of generic binding reagents. J. Immunol. Methods 167: 173-182.
52. SHIBUI, T., K. MUNAKATA, R. MATSUMOTO, K. OHTA, R. MATSUSHIMA, Y. MORIMOTO & K. NAGAHARI. 1993. High-level production and secretion of a mouse chimeric Fab fragment with specificity to human carcino embryonic antigen in Escherichia coli. Appl. Microbiol. Biotechnol. 38: 770-775.
53. YEE, L. & H. W. BLANCH. 1992. Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli. Bio/Technology 10: 1550-1556.
54. PAN, J. G., J. S. RHEE & J. M. LEBEAULT. 1987. Physiological constraints in increasing biomass concentration of Escherichia coli B in fed-batch culture Biotechnol. Lett. 9: 89-94.
55. SHIMIZU, N., S. FUKUZONO, K. FUJIMORI, N. NISHIMURA & Y. ODAWARA. 1988. Fed-batch cultures of recombinant Escherichia coli with inhibitory substance concentration monitoring. J. Ferment. Technol. 66: 187-191.
56. JENSEN, E. B. & S. CARLSEN. 1990. Production of recombinant human growth hormone in Escherichia coli : Expression of different precursors and physiological effects of glucose, acetate and salts. Biotechnol. Bioeng. 36: 1-11.
57. REISENBERG, D., K. MENZEL, V. SCHULZ, K. SCHUMANN, G. VEITH, G. ZUBER & W. A. KNORRE. 1990. High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1. Appl. Microbiol. Biotechnol. 34: 77-82.
58. REISENBERG, D., V. SCHULZ, W. A. KNORRE, H.-D. POHL, D. KORZ, E. A. SANDERS, A. ROSS & W.-D. DECKWER. 1991. High cell density cultivation of Escherichia coli at controlled specific growth rate. J. Biotechnol. 20: 17-28.
59. SHIBUI, T. & K. NAGAHARI. 1992. Secretion of a functional Fab fragment in Escherichia coli and the influence of culture conditions. Appl. Microbiol. Biotechnol. 37: 352-357.
60. CABILLY, S. 1989. Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coli. Gene 85: 553-557.
61. MCGREGOR, D. P., P. E. MOLLOY, C. CUNNINGHAM & W. J. HARRIS. 1994. Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli. Mol. Immunol. 31: 219-226.
62. ZABRASKIE, D. W., D. A. WAREHEIM & M. J. POLANSKY. 1987. Effects of fermentation feeding strategies prior to induction of expression of a recombinant malaria antigen in Escherichia coli. J. Ind. Microbiol. 2: 87-95.
63. TSAI, L. B., M. MANN, F. MORRIS, C. ROTGERS & D. FENTON. 1987. The effect of organic nitrogen and glucose on the production of recombinant human insulin-like growth factor in high cell density Escherichia coli fermentations. J. Ind. Microbiol. 2: 181-187.
64. NANCIB, N., C. BRANLANT & J. BOUDRANT. 1991. Metabolic roles of peptone and yeast extract for the culture of a recombinant strain of Escherichia coli. J. Ind. Microbiol. 8: 165-170.
65. H-domain in Escherichia coli using the pelB secretion signal. Gene 113: 95-99.
66. LI, X., J. W. ROBBINS, JR. & K. B. TAYLOR. 1990. The production of recombinant beta-galactosidase in Escherichia coli in yeast extract enriched medium. J. Ind. Microbiol. 5: 85-94.
67. MACKENZIE, C. R., V. SHARMA, D. BRUMMELL, D. BILOUS, G. DUBUC, J. SADOWSKA, N. M. YOUNG, D. R. BUNDLE & S. A. NARANG. 1994. Effect of Cλ-Cκ domain switching on Fab activity and yield in Escherichia coli: Synthesis and expression eigenes encoding two anti-carbohydrate Fabs. Bio/Technology 12: 390-395.
68. SOMERVILLE, JR. J. E., S. C. GOSHORN, H. P. FELL & R. P. DARVEAU. 1994. Bacterial aspects associated with the expression of a single-chain antibody fragment in Escherichia coli. Appl. Microbiol. Biotechnol. 42: 595-603.
69. ANNAND, N. N., S. MANDAL, C. R. MACKENZIE, J. SADOWSKA, B. SIGURSKJOLD, N. M. YOUNG, D. R. BUNDLE & S. NARANG. 1991. Bacterial expression and secretion of various single chain Fv genes encoding proteins specific for a Salmonella serotype B O-antigen. J. Biol. Chem. 266: 21874-21879.
70. ALFTHAN, K., K. TAKKINEN, D. SIZMANN, I. SEPPALA, T. IMMONEN, L. VANNE, S. KERANEN, M. KAARTINEN, J. K. C. KNOWLES & T. T. TEERI. 1993. Efficient secretion of murine Fab fragments by Escherichia coli is determined by the first constant domain of the heavy chain. Gene 128: 203-209.
71. TAKAGI, H., Y. MORINAGA, M. TSUCHIYA, H. IKEMURA & M. INOUYE. 1988. Control of folding of proteins secreted by a high expression secretion vector, pIN-III-ompA: 16-fold increase in production of active subtilisin E in Escherichia coli. Bio/Technology 6: 948-950.
72. FROYEN, G., I. RONSSE & A. BILLIAU. 1993. Bacterial expression of a single-chain antibody fragment (scFv) that neutralizes the biological activity of human interferon-γ molecular. Immunology 30: 805-812.
73. CHAUDHARY, V. K., J. K. BATRA, M. G. GALLO, M. C. WILLINGHAM, D. J. FITZGERALD & I. PASTAN. 1990. A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins. PNAS 87: 1066-1070.
74. ANTHONY, J., R. NEAR, S.-L. WONG, E. IIDA, E. ERNST, M. WITTEKIND, E. HABER & S.-C. NG. 1992. Production of stable anti-digoxin Fv in Escherichia coli. Mol. Immunol. 29: 1237-1247.
75. TSUMOTO, K., Y. NAKAOKI, Y. UEDA, K. OGASAHARA, K. YUTANI, K. WATANABE & I. KUMAGAI. 1994. Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties. Biochem. Biophys. Res. Commun. 201: 546-551.
76. JOHNSON, S. & R. E. BIRD. 1991. Construction of single-chain Fv derivatives of monoclonal antibodies and their production in Escherichia coli. Methods Enzymol. 203: 88-98.
77. POHLNER, J., J. KRAMER & T. F. MEYER. 1993. A plasmid system for high-level expression and in vitro processing of recombinant proteins. Gene 130: 121-126.
78. SKERRA, A. 1994. Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151: 131-135.
79. SKERRA, A. 1994. A general vector, pSAK84, for cloning, bacterial production and single-step purification of antibody Fab fragments. Gene 141: 79-84.
80. CUMBER, A. J., E. S. WARD, G. WINTER, G. D. PARNELL & E. J. WAWRZYNCZAK. 1992. Comparative stabilities in vitro and in vivo of a recombinant mouse antibody FvCys fragment and a bisFvCys conjugate. J. Immunol. 149: 120-126.
81. NYGREN, P.-A., A. KONDO, B. PERSSON & M. UHLEN. 1993. Stability of antibody fragments secreted to the culture medium of Escherichia coli. Protein Eng. 6(SS): 92.