Investigation of the impact of Tat export pathway enhancement on E. coli culture, protein production and early stage recovery

Biotechnology and Bioengineering

Volumn 109, Issue 4, 2012, Pages 983-991

  Branston, Steven D ^a   Matos, Cristina F R O ^b   Freedman, Robert B ^b   Robinson, Colin ^b   Keshavarz Moore, Eli ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Cell engineering; Clarification; Fermentation; Robustness; Tat pathway

Indexed keywords

BIOPROCESSES; CELL INTEGRITY; DETRIMENTAL EFFECTS; DOWNSTREAM-PROCESSING; E. COLI; FOLDED PROTEINS; GROWTH MEDIUM; INNER MEMBRANES; INTACT CELLS; MEMBRANE INTEGRITY; MEMBRANE PROTEINS; OVER-EXPRESSION; PERIPLASMIC ACCUMULATION; PROTEIN ACCUMULATION; PROTEIN PRODUCTION; SCALE-DOWN; SCALEABLE; SPHEROPLASTS; SUBSTRATE PROTEINS; TAT PATHWAY; TWIN-ARGININE TRANSLOCATIONS; WILD TYPES;

AMINO ACIDS; BIOLOGICAL MEMBRANES; CELL CULTURE; CELLS; CENTRIFUGATION; CLARIFICATION; ESCHERICHIA COLI; EXPERIMENTS; FERMENTATION; MOLECULAR BIOLOGY; PROTEINS; ROBUSTNESS (CONTROL SYSTEMS); SHEAR FLOW;

CELL ENGINEERING;

MEMBRANE PROTEIN; PERIPLASMIC PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL ENGINEERING; CELL MEMBRANE; CENTRIFUGATION; CONTROLLED STUDY; DOWNSTREAM PROCESSING; ESCHERICHIA COLI; FERMENTATION; GROWTH RATE; LYSOSOME; NONHUMAN; OSMOTIC STRESS; PROTEIN EXPRESSION; PROTEIN FOLDING; SIGNAL TRANSDUCTION; SPHEROPLAST; TWIN ARGININE TRANSLOCATION PATHWAY;

AMINO ACID SEQUENCE; BACTERIAL TRANSLOCATION; BACTERIOLOGICAL TECHNIQUES; BATCH CELL CULTURE TECHNIQUES; BIOLOGICAL TRANSPORT, ACTIVE; CELL MEMBRANE; CENTRIFUGATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; INDUSTRIAL MICROBIOLOGY; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; PERIPLASM; PERIPLASMIC BINDING PROTEINS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SPHEROPLASTS; VISCOSITY;

EID: 84857445113     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24384     Document Type: Article

References (38)

1. Balasundaram B, Harrison S, Bracewell DG. 2009a. Advances in product release strategies and impact on bioprocess design. Trends Biotechnol 27(8):477-485.
2. Balasundaram B, Nesbeth D, Ward JM, Keshavarz Moore E, Bracewell DG. 2009b. Step change in the efficiency of centrifugation through cell engineering: Coexpression of Staphylococcal nuclease to reduce the viscosity of the bioprocess feedstock. Biotechnol Bioeng 104(1):134-142.
3. Barrett CML, Ray N, Thomas JD, Robinson C, Bolhuis A. 2003. Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli. Biochem Biophys Res Commun 304(2):279-284.
4. Birdsell DC, Cota-Robles EH. 1967. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J Bacteriol 93(1):427-437.
5. Bolhuis A, Bogsch EG, Robinson C. 2000. Subunit interactions in the twin-arginine translocase complex of Escherichia coli. FEBS Lett 472(1):88-892.
6. Bolhuis A, Mathers JE, Thomas JD, Barrett CML, Robinson C. 2001. TatB and TatC form a functional and structural unit of the twin-arginine translocase from Escherichia coli. J Biol Chem 276(23):20213-20219.
7. Bowering LC. 2000. An engineering study of the design, integration, and control of antibody fragment production processes. London: University College London.
8. Boychyn M, Yim SSS, Bulmer M, More J, Bracewell DG, Hoare M. 2004. Performance prediction of industrial centrifuges using scale-down models. Bioproc Biosyst Eng 26(6):385-391.
9. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1-2):248-254.
10. Casadaban MJ, Cohen SN. 1979. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: In vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76(9):4530-4533.
11. Dykxhoorn DM, St. Pierre R, Linn T. 1996. A set of compatible tac promoter expression vectors. Gene 177(1-2):133-136.
12. Garcia-Arrazola R, Siu S, Chan G, Buchanan I, Doyle B, Titchener-Hooker N, Baganz F. 2005. Evaluation of a pH-stat feeding strategy on the production and recovery of Fab fragments from E. coli. Biochem Eng J 23(3):221-230.
13. Georgiou G, Segatori L. 2005. Preparative expression of secreted proteins in bacteria: Status report and future prospects. Curr Opin Biotechnol 16(5):538-545.
14. Graumann K, Premstaller A. 2006. Manufacturing of recombinant therapeutic proteins in microbial systems. Biotechnol J 1(2):164-186.
15. Guzman LM, Belin D, Carson MJ, Beckwith JON. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177(14):4121-4130.
16. Harrison J, Keshavarz-Moore E. 1996. Production of antibody fragments in Escherichia coli. Ann N Y Acad Sci 782:143-158.
17. Heydarian SM, Ison AP, Lilly MD, Shamlou PA. 2000. Turbulent breakage of filamentous bacteria in mechanically agitated batch culture. Chem Eng Sci 55:1775-1784.
18. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. 2006. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng 95(3):483-491.
19. Ize B, Stanley NR, Buchanan G, Palmer T. 2003. Role of the Escherichia coli Tat pathway in outer membrane integrity. Mol Microbiol 48(5):1183-1193.
20. Ize B, Porcelli I, Lucchini S, Hinton JC, Berks BC, Palmer T. 2004. Novel phenotypes of Escherichia coli tat mutants revealed by global gene expression and phenotypic analysis. J Biol Chem 279(46):47543-47554.
21. Matos CFRO, Di Cola A, Robinson C. 2009. TatD is a central component of a Tat translocon-initiated quality control system for exported FeS proteins in Escherichia coli. EMBO Rep 10(5):474-479.
22. Mollet M, Ma N, Zhao Y, Brodkey R, Taticek R, Chalmers JJ. 2004. Bioprocess equipment: Characterization of energy dissipation rate and its potential to damage cells. Biotechnol Prog 20(5):1437-1448.
23. Neal G, Christie J, Keshavarz-Moore E, Shamlou PA. 2003. Ultra scale-down approach for the prediction of full-scale recovery of ovine polycolonal immunoglobulins used in the manufacture of snake venom-specific Fab fragment. Biotechnol Bioeng 81(2):149-157.
24. Pierce JJ, Turner C, Keshavarz-Moore E, Dunnill P. 1997. Factors determining more efficient large-scale release of a periplasmic enzyme from E. coli using lysozyme. J Biotechnol 58(1):1-11.
25. Randall LL, Hardy SJS. 1986. Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli. Cell 46(6):921-928.
26. Robinson C, Bolhuis A. 2004. Tat-dependent protein targeting in prokaryotes and chloroplasts. Biochim Biophys Acta (BBA) Mol Cell Res 1694(1-3):135-147.
27. Sargent F, Bogsch EG, Stanley NR, Wexler M, Robinson C, Berks BC, Palmer T. 1998. Overlapping functions of components of a bacterial Sec-independent protein export pathway. EMBO J 17(13):3640-3650.
28. Settles AM, Yonetani A, Baron A, Bush DR, Cline K, Martienssen R. 1997. Sec-independent protein translocation by the maize Hcf106 protein. Science 278(5342):1467-1470.
29. Tait AS, Aucamp JP, Bugeon A, Hoare M. 2009. Ultra scale-down prediction using microwell technology of the industrial scale clarification characteristics by centrifugation of mammalian cell broths. Biotechnol Bioeng 104(2):321-331.
30. Thomas JD, Daniel RA, Errington J, Robinson C. 2001. Export of active green fluorescent protein to the periplasm by the twin arginine translocase (Tat) pathway in Escherichia coli. Mol Microbiol 39(1):47-53.
31. Tustian AD, Salte H, Willoughby NA, Hassan I, Rose MH, Baganz F, Hoare M, Titchener-Hooker NJ. 2007. Adapted ultra scale-down approach for predicting the centrifugal separation behavior of high cell density cultures. Biotechnol Prog 23(6):1404-1410.
32. Wagner S, Bader ML, Drew D, de Gier J-W. 2006. Rationalizing membrane protein overexpression. Trends Biotechnol 24(8):364-371.
33. Walsh G. 2006. Biopharmaceutical benchmarks 2006. Nat Biotechnol 24(7):769-776.
34. Walsh G. 2010. Post-translational modifications of protein biopharmaceuticals. Drug Discov Today 15(17-18):773-780.
35. Weiner JH, Bilous PT, Shaw GM, Lubitz SP, Frost L, Thomas GH, Cole JA, Turner RJ. 1998. A novel and ubiquitous system for membrane targeting and secretion of cofactor-containing proteins. Cell 93(1):93-101.
36. Wexler M, Sargent F, Jack RL, Stanley NR, Bogsch EG, Robinson C, Berks BC, Palmer T. 2000. TatD is a cytoplasmic protein with DNase activity. J Biol Chem 275(22):16717-16722.
37. Yim SSS, Shamlou PA. 2000. The engineering effects of fluids flow on freely suspended biological macro-materials and macromolecules. Adv Biochem Eng Biotechnol 67:83-122.
38. Zhang H, Kong S, Booth A, Boushaba R, Levy MS, Hoare M. 2007. Prediction of shear damage of plasmid DNA in pump and centrifuge operations using an ultra scale-down device. Biotechnol Prog 23(4):858-865.