Tissue processing prior to analysis of alzheimer's disease associated proteins and metabolites, including Aβ

Methods in Molecular Biology

Volumn 849, Issue , 2012, Pages 493-506

  Schmidt, Stephen D ^a,b   Nixon, Ralph A ^a,c   Mathews, Paul M ^a,c  

^a NATHAN S KLINE INSTITUTE FOR PSYCHIATRIC RESEARCH   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

A ; Alzheimer's disease; Amyloid; Amyloid precursor protein; APP; Brain; Dissection; Fractionation; Homogenization; Membrane protein; SDS PAGE; Western blot analysis

Indexed keywords

AMYLOID BETA PROTEIN; AMYLOID PRECURSOR PROTEIN; MEMBRANE PROTEIN; PEPTIDE FRAGMENT; SUCROSE;

ALZHEIMER DISEASE; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR SPACE; HEMISPHERE; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; TISSUE HOMOGENATE; TISSUE PREPARATION; TRANSGENIC MOUSE; WESTERN BLOTTING; ANIMAL; BRAIN; CHEMISTRY; DISSECTION; HUMAN; METABOLISM; PATHOLOGY; SOLUBILITY; TISSUE FIXATION;

ALZHEIMER DISEASE; AMYLOID BETA-PROTEIN PRECURSOR; ANIMALS; BLOTTING, WESTERN; BRAIN; DISSECTION; HUMANS; IMMUNOHISTOCHEMISTRY; MEMBRANE PROTEINS; MICE; PEPTIDE FRAGMENTS; SOLUBILITY; SUCROSE; TISSUE FIXATION;

EID: 84864612024     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-551-0_33     Document Type: Article

References (29)

1. Gravina, S. A., Ho, L., Eckman, C. B., Long, K. E., Otvos, L., Jr., Younkin, L. H., Suzuki, N., and Younkin, S. G. (1995) Amyloid beta protein (A beta) in Alzheimer's disease brain. Biochemical and immunocytochemical analysis with antibodies specifi c for forms ending at A beta 40 or A beta 42(43), J Biol Chem 270 , 7013-7016
2. Janus, C., Pearson, J., McLaurin, J., Mathews, P. M., Jiang, Y., Schmidt, S. D., Chishti, M. A., Horne, P., Heslin, D., French, J., Mount, H. T., Nixon, R. A., Mercken, M., Bergeron, C., Fraser, P. E., St George-Hyslop, P., and Westaway, D. (2000) A beta peptide immunization reduces behavioural impairment and plaques in a model of Alzheimer's disease, Nature 408 , 979-982
3. Refolo, L. M., Pappolla, M. A., LaFrancois, J., Malester, B., Schmidt, S. D., Thomas-Bryant, T., Tint, G. S., Wang, R., Mercken, M., Petanceska, S. S., and Duff, K. E. (2001) A cholesterol-lowering drug reduces beta-amyloid pathology in a transgenic mouse model of Alzheimer's disease, Neurobiol Dis 8 , 890-899
4. Rozmahel, R., Huang, J., Chen, F., Liang, Y., Nguyen, V., Ikeda, M., Levesque, G., Yu, G., Nishimura, M., Mathews, P., Schmidt, S. D., Mercken, M., Bergeron, C., Westaway, D., and St George-Hyslop, P. (2002) Normal brain development in PS1 hypomorphic mice with markedly reduced gamma-secretase cleavage of betaAPP, Neurobiol Aging 23 , 187-194
5. Rozmahel, R., Mount, H. T., Chen, F., Nguyen, V., Huang, J., Erdebil, S., Liauw, J., Yu, G., Hasegawa, H., Gu, Y., Song, Y. Q., Schmidt, S. D., Nixon, R. A., Mathews, P. M., Bergeron, C., Fraser, P., Westaway, D., and George-Hyslop, P. S. (2002) Alleles at the Nicastrin locus modify presenilin 1-defi ciency phenotype, Proc Natl Acad Sci USA 99 , 14452-14457
6. Pfeifer, M., Boncristiano, S., Bondolfi , L., Stalder, A., Deller, T., Staufenbiel, M., Mathews, P. M., and Jucker, M. (2002) Cerebral hemorrhage after passive anti-Abeta immunotherapy, Science 298 , 1379
7. Phinney, A. L., Drisaldi, B., Schmidt, S. D., Lugowski, S., Coronado, V., Liang, Y., Horne, P., Yang, J., Sekoulidis, J., Coomaraswamy, J., Chishti, M. A., Cox, D. W., Mathews, P. M., Nixon, R. A., Carlson, G. A., St George-Hyslop, P., and Westaway, D. (2003) In vivo reduction of amyloid-beta by a mutant copper transporter, Proc Natl Acad Sci USA 100 , 14193-14198
8. Herzig, M. C., Winkler, D. T., Burgermeister, P., Pfeifer, M., Kohler, E., Schmidt, S. D., Danner, S., Abramowski, D., Sturchler-Pierrat, C., Burki, K., van Duinen, S. G., Maat-Schieman, M. L., Staufenbiel, M., Mathews, P. M., and Jucker, M. (2004) Abeta is targeted to the vasculature in a mouse model of hereditary cerebral hemorrhage with amyloidosis, Nat Neurosci 7 , 954-960
9. Lacombe, P., Mathews, P. M., Schmidt, S. D., Breidert, T., Heneka, M. T., Landreth, G. E., Feinstein, D. L., and Galea, E. (2004) Effect of anti-infl ammatory agents on transforming growth factor beta over-expressing mouse brains: A model revised, J Neuroinfl ammation
10. Pawlik, M., Sastre, M., Calero, M., Mathews, P. M., Schmidt, S. D., Nixon, R. A., and Levy, E. (2004) Overexpression of human cystatin C in transgenic mice does not affect levels of endogenous brain amyloid Beta Peptide, J Mol Neurosci 22 , 13-18
11. Yao, J., Petanceska, S. S., Montine, T. J., Holtzman, D. M., Schmidt, S. D., Parker, C. A., Callahan, M. J., Lipinski, W. J., Bisgaier, C. L., Turner, B. A., Nixon, R. A., Martins, R. N., Ouimet, C., Smith, J. D., Davies, P., Laska, E., Ehrlich, M. E., Walker, L. C., Mathews, P. M., and Gandy, S. (2004) Aging, gender and APOE isotype modulate metabolism of Alzheimer's Abeta peptides and F-isoprostanes in the absence of detectable amyloid deposits, J Neurochem 90 , 1011-1018
12. Mastrangelo, P., Mathews, P. M., Chishti, M. A., Schmidt, S. D., Gu, Y., Yang, J., Mazzella, M. J., Coomaraswamy, J., Horne, P., Strome, B., Pelly, H., Levesque, G., Ebeling, C., Jiang, Y., Nixon, R. A., Rozmahel, R., Fraser, P. E., St George-Hyslop, P., Carlson, G. A., and Westaway, D. (2005) Dissociated phenotypes in presenilin transgenic mice defi ne functionally distinct gamma-secretases, Proc Natl Acad Sci USA 102 , 8972-8977
13. Gandy, S., Zhang, Y. W., Ikin, A., Schmidt, S. D., Bogush, A., Levy, E., Sheffi eld, R., Nixon, R. A., Liao, F. F., Mathews, P. M., Xu, H., and Ehrlich, M. E. (2007) Alzheimer's presenilin 1 modulates sorting of APP and its carboxylterminal fragments in cerebral neurons in vivo, J Neurochem 102 , 619-626
14. Mi, W., Pawlik, M., Sastre, M., Jung, S. S., Radvinsky, D. S., Klein, A. M., Sommer, J., Schmidt, S. D., Nixon, R. A., Mathews, P. M., and Levy, E. (2007) Cystatin C inhibits amyloid-beta deposition in Alzheimer's disease mouse models, Nat Genet 39 , 1440-1442
15. Trinchese, F., Fa, M., Liu, S., Zhang, H., Hidalgo, A., Schmidt, S. D., Yamaguchi, H., Yoshii, N., Mathews, P. M., Nixon, R. A., and Arancio, O. (2008) Inhibition of calpains improves memory and synaptic transmission in a mouse model of Alzheimer disease, J Clin Invest 118 , 2796-2807
16. Yang, D. S., Stavrides, P., Mohan, P. S., Kaushik, S., Kumar, A., Ohno, M., Schmidt, S. D., Wesson, D., Bandyopadhyay, U., Jiang, Y., Pawlik, M., Peterhoff, C. M., Yang, A. J., Wilson, D. A., St George-Hyslop, P., Westaway, D., Mathews, P. M., Levy, E., Cuervo, A. M., and Nixon, R. A. (2011) Reversal of autophagy dysfunction in the TgCRND8 mouse model of Alzheimer's disease ameliorates amyloid pathologies and memory defi cits, Brain 134 , 258-277
17. Choi, J. H., Berger, J. D., Mazzella, M. J., Morales-Corraliza, J., Cataldo, A. M., Nixon, R. A., Ginsberg, S. D., Levy, E., and Mathews, P. M. (2009) Age-dependent dysregulation of brain amyloid precursor protein in the Ts65Dn Down syndrome mouse model, J Neurochem 110 , 1818-1827
18. Morales-Corraliza, J., Mazzella, M. J., Berger, J. D., Diaz, N. S., Choi, J. H., Levy, E., Matsuoka, Y., Planel, E., and Mathews, P. M. (2009) In vivo turnover of tau and APP metabolites in the brains of wild-type and Tg2576 mice: greater stability of sAPP in the beta-amyloid depositing mice, PLoS One 4 , e7134
19. Weidemann, A., Konig, G., Bunke, D., Fischer, P., Salbaum, J. M., Masters, C. L., and Beyreuther, K. (1989) Identifi cation, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein, Cell 57 , 115-126
20. Golde, T. E., Estus, S., Younkin, L. H., Selkoe, D. J., and Younkin, S. G. (1992) Processing of the amyloid protein precursor to potentially amyloidogenic derivatives, Science 255 , 728-730
21. Seubert, P., Oltersdorf, T., Lee, M. G., Barbour, R., Blomquist, C., Davis, D. L., Bryant, K., Fritz, L. C., Galasko, D., Thal, L. J., and et al. (1993) Secretion of beta-amyloid precursor protein cleaved at the amino terminus of the beta-amyloid peptide, Nature 361 , 260-263
22. Caporaso, G. L., Gandy, S. E., Buxbaum, J. D., Ramabhadran, T. V., and Greengard, P. (1992) Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein, Proc Natl Acad Sci USA 89 , 3055-3059
23. Mathews, P. M., Jiang, Y., Schmidt, S. D., Grbovic, O. M., Mercken, M., and Nixon, R. A. (2002) Calpain activity regulates the cell surface distribution of amyloid precursor protein: inhibition of calpains enhances endosomal generation of beta-cleaved C-terminal APP fragments, J Biol Chem 277 , 36415-36424
24. Mathews, P. M., Guerra, C. B., Jiang, Y., Grbovic, O. M., Kao, B. H., Schmidt, S. D., Dinakar, R., Mercken, M., Hille-Rehfeld, A., Rohrer, J., Mehta, P., Cataldo, A. M., and Nixon, R. A. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis, J Biol Chem 277 , 5299-5307
25. Mathews, P. M., Cataldo, A. M., Kao, B. H., Rudnicki, A. G., Qin, X., Yang, J. L., Jiang, Y., Picciano, M., Hulette, C., Lippa, C. F., Bird, T. D., Nochlin, D., Walter, J., Haass, C., Levesque, L., Fraser, P. E., Andreadis, A., and Nixon, R. A. (2000) Brain expression of presenilins in sporadic and early-onset, familial Alzheimer's disease, Mol Med 6 , 878-891
26. Duff, K., Eckman, C., Zehr, C., Yu, X., Prada, C. M., Perez-tur, J., Hutton, M., Buee, L., Harigaya, Y., Yager, D., Morgan, D., Gordon, M. N., Holcomb, L., Refolo, L., Zenk, B., Hardy, J., and Younkin, S. (1996) Increased amyloid-beta42(43) in brains of mice expressing mutant presenilin 1, Nature 383 , 710-713
27. Hsiao, K., Chapman, P., Nilsen, S., Eckman, C., Harigaya, Y., Younkin, S., Yang, F., and Cole, G. (1996) Correlative memory defi cits, Abeta elevation, and amyloid plaques in transgenic mice, Science 274 , 99-102
28. Kosik, K. S., Orecchio, L. D., Binder, L., Trojanowski, J. Q., Lee, V. M., and Lee, G. (1988) Epitopes that span the tau molecule are shared with paired helical fi laments, Neuron 1 , 817-825
29. Hilbich, C., Monning, U., Grund, C., Masters, C. L., and Beyreuther, K. (1993) Amyloid-like properties of peptides fl anking the epitope of amyloid precursor protein-specifi c monoclonal antibody 22C11, J Biol Chem 268 , 26571-26577.