Expression of a library of fungal ß-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain

Applied Microbiology and Biotechnology

Volumn 95, Issue 3, 2012, Pages 647-659

  Wilde, Caroline ^a,b   Gold, Nicholas D ^a   Bawa, Nancy ^a   Tambor, José Humberto M ^a   Mougharbel, Lina ^a   Storms, Reginald ^a   Martin, Vincent J J ^a  

^a Gina Cody School of Engineering and Computer Science   (Canada)

^b LALLEMAND SAS   (France)

Author keywords

Glucosidase; Cell surface display; Ethanol; Saccharomyces cerevisiae

Indexed keywords

ACTIVITY ASSAYS; ASPERGILLUS NIGER; CELL POPULATIONS; CELL SURFACE DISPLAYS; CELL SURFACES; CELLOBIOSE; CELLULOSIC BIOMASS; CHROMOSOMAL INTEGRATION; DIFFERENT SUBSTRATES; ETHANOL PRODUCTION; ETHANOL YIELD; EXPRESSION LEVELS; FLOW CYTOMETRY ANALYSIS; GLUCOSIDASE; NATURAL SUBSTRATES; S.CEREVISIAE; SPECIFIC GROWTH RATE; YEAST SACCHAROMYCES CEREVISIAE;

ASPERGILLUS; CELL CULTURE; CELL MEMBRANES; CELL PROLIFERATION; CELLULOSE; CHROMOSOMES; ENZYMATIC HYDROLYSIS; ETHANOL; GLUCOSE;

YEAST;

BETA GLUCOSIDASE; CELLOBIOSE; CELLULOSE;

BIOASSAY; BIOLOGICAL PRODUCTION; BIOMASS; CELLULOSE; CHROMOSOME; ENZYME ACTIVITY; ETHANOL; FERMENTATION; FUNGUS; GENE EXPRESSION; GLUCOSE; HYDROLYSIS;

ALCOHOL PRODUCTION; ARTICLE; ASPERGILLUS NIGER; ASSAY; BIOMASS FERMENTATION; CELL POPULATION; CELL SURFACE; CHROMOSOME; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGUS GROWTH; HYDROLYSIS; NONHUMAN; PLASMID; PROTEIN ANALYSIS; SACCHAROMYCES CEREVISIAE; TITRIMETRY;

ASPERGILLUS NIGER; BIOMASS; CELLULASES; CELLULOSE; ETHANOL; FERMENTATION; GENE EXPRESSION; GENE LIBRARY; METABOLIC ENGINEERING; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

ASPERGILLUS NIGER; SACCHAROMYCES CEREVISIAE;

EID: 84864586492     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3788-z     Document Type: Article

References (50)

1. Almeida JRM, Modig T, Petersson A, Hahn-Hägerdal B, Lidén G, Gorwa-Grauslund MF (2007) Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J Chem Technol Biotechnol 82:340-349
2. Araujo EF, Barros EG, Caldas RA, Silva DO (1983) Beta-glucosidase activity of a thermophilic cellulolytic fungus, Humicola sp. Biotechnol Lett 5:781-784
3. Breinig F, Schmitt MJ (2002) Spacer-elongated cell wall fusion proteins improve cell surface expression in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 58:637-44
4. Calsavara L, De Moraes F, Zanin G (2001) Comparison of catalytic properties of free and immobilized cellobiase Novozym 188. Appl Biochem Biotechnol 91-93:615-626
5. Dan S, Marton I, Dekel M, Bravdo B-A, He S, Withers SG, Shoseyov O (2000) Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger ß-glucosidase. J Biol Chem 275:4973-4980
6. Den Haan R, Rose SH, Lynd LR, Van Zyl WH (2007) Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng 9:87-94
7. Du Plessis L, Rose SH, Van Zyl WH (2010) Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 86:1503-1511
8. Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP, and related tools. Nat Protoc 2:953-971
9. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A (2004) Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl Environ Microbiol 70:1207-1212
10. Galazka JM, Tian CG, Beeson WT, Martinez B, Glass NL, Cate JHD (2010) Cellodextrin transport in yeast for improved biofuel production. Science 330:84-86
11. Gietz RD, Schiestl RH (1995) Transforming yeast with DNA. Meth Mol Cell Biol 5:255-269
12. Goujon M, McWilliam H, Li WZ, Valentin F, Squizzato S, Paern J, Lopez R (2010) A new bioinformatics analysis tools framework at EMBL-EBI. Nucleic Acids Res 38:W695-W699
13. Guo ZP, Zhang L, Ding ZY, Gu ZH, Shi GY (2011) Development of an industrial ethanol-producing yeast strain for efficient utilization of cellobiose. Enzyme Microb Tech 49:105-112
14. Hamilton R, Watanabe CK, de Boer HA (1987) Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mrnas. Nucleic Acids Res 15:3581-3593
15. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557-580
16. Harju S, Fedosyuk H, Peterson KR (2004) Rapid isolation of yeast genomic DNA: Bust n' grab. BMC Biotechnol 4:8
17. Henrissat B, Driguez H, Viet C, Schülein M (1985) Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Nat Biotechnol 3:722-726
18. Himmel ME, Adney WS, Fox JW, Mitchell DJ, Baker JO (1993) Isolation and characterization of two forms of ß-D-glucosidase from Aspergillus niger. Appl Biochem Biotechnol 39-40:213-25
19. Hoh YK, Yeoh HH, Tan TK (1992) Properties of ß-glucosidase purified from Aspergillus niger mutants USDB 0827 and USDB 0828. Appl Microbiol Biotechnol 37:590-593
20. Jeon E, Hyeon JE, Eun LS, Park BS, Kim SW, Lee J, Han SO (2009a) Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera ß-glucosidase. FEMS Microbiol Lett 301:130-136
21. Jeon E, Hyeon JE, Suh DJ, Suh YW, Kim SW, Song KH, Han SO (2009b) Production of cellulosic ethanol in Saccharomyces cerevisiae heterologous expressing Clostridium thermocellum endoglucanase and Saccharomycopsis fibuligera ß-glucosidase genes. Mol Cells 28:369-373
22. Kondo A, Ueda M (2004) Yeast cell-surface display-applications of molecular display. Appl Microbiol Biotechnol 64:28-40
23. Kotaka A, Bando H, Kaya M, Kato-Murai M, Kuroda K, Sahara H, Hata Y, Kondo A, Ueda M (2008) Direct ethanol production from barley ß-glucan by sake yeast displaying Aspergillus oryzae ß-glucosidase and endoglucanase. J Biosci Bioeng 105:622-627
24. Kotaka A, Sahara H, Kuroda K, Kondo A, Ueda M, Hata Y (2010) Enhancement of ß-glucosidase activity on the cell-surface of sake yeast by disruption of SED1. J Biosci Bioeng 109:442-446
25. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) ClustalW and ClustalX version 2. Bioinformatics 23:2947-2948
26. Lynd LR, Weimer PJ, Van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66:506-577
27. Lynd LR, van Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16:577-83
28. Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, Himmel M, Keller M, McMillan JD, Sheehan J, Wyman CE (2008) How biotech can transform biofuels. Nature Biotechnol 26:169-172
29. Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, He S, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Tasneem A, Thanki N, Yamashita RA, Zhang D, Zhang N, Bryant SH (2009) CDD: Specific functional annotation with the conserved domain database. Nucleic Acids Res 37:205-210
30. Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Lu F, Marchler GH, Mullokandov M, Omelchenko MV, Robertson CL, Song JS, Thanki N, Yamashita RA, Zhang D, Zhang N, Zheng C, Bryant SH (2011) CDD: A conserved domain database for the functional annotation of proteins. Nucleic Acids Res 39:D225-D229
31. Newman JR, Ghaemmaghami S, Ihmels J, Breslow DK, Noble M, DeRisi JL, Weissman JS (2006) Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise. Nature 441:840-6
32. Oliveira C, Teixeira JA, Lima N, Da Silva NA, Domingues L (2007) Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger ß-galactosidase production. J Biosci Bioeng 103:318-324
33. Pack SP, Park K, Yoo YJ (2002) Enhancement of ß-glucosidase stability and cellobiose-usage using surface-engineered recombinant Saccharomyces cerevisiae in ethanol production. Biotechnol Lett 24:1919-1925
34. Parry NJ, Beever DE, Owen E, Vandenberghe I, Van Beeumen J, Bhat MK (2001) Biochemical characterization and mechanism of action of a thermostable ß-glucosidase purified from Thermoascus aurantiacus. Biochem J 353:117-127
35. Riou C, Salmon JM, Vallier MJ, Gunata Z, Barre P (1998) Purification, characterization, and substrate specificity of a novel highly glucose-tolerant ß-glucosidase from Aspergillus oryzae. Appl Environ Microbiol 64:3607-3614
36. Romanos MA, Scorer CA, Clare JJ (1992) Foreign gene expression in yeast: a review. Yeast 8:423-488
37. Sakai A, Shimizu Y, Hishinuma F (1990) Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty. Appl Microbiol Biotechnol 33:302-306
38. Seidle HF, Marten I, Shoseyov O, Huber RE (2004) Physical and kinetic properties of the family 3 ß-glucosidase from Aspergillus niger which is important for cellulose breakdown. Protein J 23:11-23
39. Shibasaki S, Maeda H, Ueda M (2009) Molecular display technology using yeast-arming technology. Anal Sci 25:41-49
40. Sonderegger M, Jeppsson M, Hahn-Hägerdal B, Sauer U (2004) Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis. Appl Environ Microbiol 70:2307-2317
41. Tsai SL, Goyal G, Chen W (2010) Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and ethanol production. Appl Environ Microbiol 76:7514-20
42. Van der Vaart JM, Te Biesebeke R, Chapman JW, Toschka HY, Klis FM, Verrips T (1997) Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins. Appl Environ Microbiol 63:615-620
43. Van Hoek P, Van Dijken JP, Pronk JT (1998) Effect of specific growth rate on fermentative capacity of baker's yeast. Appl Environ Microbiol 64:4226-4233
44. Van Maris AJA, Winkler AA, Kuyper M, de Laat WTAM, Van Dijken JP, Pronk JT (2007) Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component. Adv Biochem Eng Biotechnol 108:179-204
45. Van Rensburg P, Van Zyl WH, Pretorius IS (1998) Engineering yeast for efficient cellulose degradation. Yeast 14:67-76
46. Van Rooyen R, Hahn-Hägerdal B, La Grange DC, Van Zyl WH (2005) Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains. J Biotechnol 120:284-295
47. Visser W, Scheffers WA, Batenburg-Van der Vegte WH, Van Dijken JP (1990) Oxygen requirements of yeasts. Appl Environ Microbiol 56:3785-3792
48. Yang B, Wyman CE (2008) Pretreatment: the key to unlocking lowcost cellulosic ethanol. Biofuels Bioprod Bior 2:26-40
49. Zhang Z, Moo-Young M, Chisti Y (1996) Plasmid stability in recombinant Saccharomyces cerevisiae. Biotechnol Adv 14:401-435
50. Zhang L, Guo Z-P, Hong J-H, Ding Z-Y, Gao Z-Q, He Z-M, Shi G-Y (2011) Expressing ß-glucosidase from Saccharomycopsis fibuligera in industrial ethanol producing yeast and evaluation of the expressing sufficiency. Ann Microbiol 61:1-6