Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme

Applied and Environmental Microbiology

Volumn 70, Issue 2, 2004, Pages 1207-1212

  Fujita, Yasuya ^a   Ito, Junji ^a   Ueda, Mitsuyoshi ^b   Fukuda, Hideki ^a   Kondo, Akihiko ^a  

^a KOBE UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATES; CELLS; CELLULOSE; ENZYMES; ETHANOL; MICROSCOPIC EXAMINATION; SACCHARIFICATION; YEAST;

CELL SURFACE DISPLAY SYSTEMS; FUSION PROTEINS; IMMUNOFLUORESCENCE;

MICROBIOLOGY;

AGGLUTININ; ALCOHOL; ALPHA AGGLUTININ; BETA GLUCOSIDASE; BETA GLUCOSIDASE 1; CARBOHYDRATE; CELLOBIOHYDROLASE II; CELLULOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; ENDOGLUCANASE II; FUNGAL ENZYME; GLUCAN SYNTHASE; HYBRID PROTEIN; PHOSPHORIC ACID; UNCLASSIFIED DRUG;

MICROBIOLOGY;

ARTICLE; ASPERGILLUS; ASPERGILLUS ACULEATUS; BIOCATALYST; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; CONTROLLED STUDY; DEGRADATION; FERMENTATION; FLUORESCENCE MICROSCOPE; FUNGAL STRAIN; HYDROLYSIS; IMMUNOFLUORESCENCE; NONHUMAN; SACCHARIFICATION; SACCHAROMYCES CEREVISIAE; TRICHODERMA REESEI; YEAST;

BETA-GLUCOSIDASE; CELLULASE; CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CULTURE MEDIA; ETHANOL; FERMENTATION; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRICHODERMA;

ASPERGILLUS; ASPERGILLUS ACULEATUS; HYPOCREA JECORINA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; TRICHODERMA;

EID: 2342638898     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.2.1207-1212.2004     Document Type: Article

References (38)

1. Aristidou, A., and M. Penttilä. 2000. Metabolic engineering applications to renewable resource utilization. Curr. Opin. Biotechnol. 11:187-198.
2. Bothast, R. J., N. N. Nichols, and B. S. Dien. 1999. Fermentations with new recombinant organisms. Biotechnol. Prog. 15:867-875.
3. Chen, H., M. Hayn, and H. Esterbauer. 1992. Purification and characterization of two extracellular β-glucosidases from Trichoderma reesei. Biochim. Biophys. Acta 1121:54-60.
4. Cho, K. M., and Y. J. Yoo. 1999. Novel SSF process for ethanol production from microcrystalline cellulose using the 8-integrated recombinant yeast, L2612δGC. J. Microbiol. Biotechnol. 9:340-345.
5. Doi, R. H., and Y. Tamaru. 2001. The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem. Rec. 1: 24-32.
6. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Reberse, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.
7. Fujita, Y., S. Takahashi, M. Ueda, A. Tanaka, H. Okada, Y. Morikawa, T. Kawaguchi, M. Arai, H. Fukuda, and A. Kondo. 2002. Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl. Environ. Microbiol. 68:5136-5141.
8. Guedon, E., M. Desvaux, and H. Petitdemange. 2002. Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. Appl. Environ. Microbiol. 68:53-58.
9. Henrissat, B., H. Driguez, C. Viet, and M. Schülein. 1985. Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Bio/Technology 3:722-726.
10. Ingram, L. O., P. F. Gomez, X. Lai, M. Moniruzzaman, B. E. Wood, L. P. Yomano, and S. W. York. 1998. Metabolic engineering of bacteria for ethanol production. Biotechnol. Bioeng. 58:204-214.
11. Lipke, P. N., D. Wojciechowicz, and J. Kurjan. 1989. AGα1 is the structural gene for the Saccharomyces cerevisiae α-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating. Mol. Cell. Biol. 9:3155-3165.
12. Lynd, L. R., C. E. Wyman, and T. U. Gerngross. 1999. Biocommodity engineering. Biotechnol. Prog. 15:777-793.
13. Medve, J., J. Karlsson, D. Lee, and F. Tjerneld. 1998. Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei: adsorption, sugar production pattern, and synergism of the enzymes. Biotechnol. Bioeng. 59:621-634.
14. Medve, J., J. Ståhlberg, and F. Tjerneld. 1994. Adsorption and synergism of cellobiohydrolase I and II of Trichoderma reesei during hydrolysis of microcrystalline cellulose. Biotechnol. Bioeng. 44:1064-1073.
15. Murai, T., M. Ueda, T. Kawaguchi, M. Arai, and A. Tanaka. 1998. Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing β-glucosidase and carboxymethylcellulase from Aspergillus aculeatus. Appl. Environ. Microbiol. 64:4857-4861.
16. Murai, T., M. Ueda, Y. Shibasaki, N. Kamasawa, M. Osumi, T. Imanaka, and A. Tanaka. 1999. Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface. Appl. Microbiol. Biotechnol. 51:65-70.
17. Okada, H., K. Tada, T. Sekiya, K. Yokoyama, A. Takahashi, H. Tohda, H. Kumagai, and Y. Morikawa. 1998. Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl. Environ. Microbiol. 64:555-563.
18. Okada, H., T. Sekiya, K. Yokoyama, H. Tohda, H. Kumagai, and Y. Morikawa. 1998. Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizozaccharomyces pombe and characterization of its products. Appl. Microbiol. Biotechnol. 49:301-308.
19. Penttilä, M., P. Lehtovaara, H. Nevalainen, R. Bhikhabhai, and J. Knowles. 1986. Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene. Gene 45:253-263.
20. Saloheimo, A., B. Henrissat, A.-M. Hoffren, O. Teleman, and M. Penttilä. 1994. A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol. 13:219-228.
21. Saloheimo, M., P. Lehtovaara, M. Penttilä, T. T. Teeri, J. Ståhlberg, G. Johansson, G. Pettersson, M. Claeyssens, P. Tomme, and J. K. C. Knowles. 1988. EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. Gene 63:11-21.
22. Saloheimo, M., T. Nakari-Setälä, M. Tenkanen, and M. Penttilä. 1997. cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast. Eur. J. Biochem. 249:584-591.
23. Shibasaki, S., M. Ueda, K. Ye, K. Shimizu, N. Kamasawa, M. Osumi, and A. Tanaka. 2001. Creation of cell surface-engineered yeast that display different fluorescent proteins in response to the glucose concentration. Appl. Microbiol. Biotechnol. 57:528-533.
24. Shibasaki, S., M. Ueda, T. Iizuka, M. Hirayama, Y. Ikeda, N. Kamasawa, M. Osumi, and A. Tanaka. 2001. Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses. Appl. Microbiol. Biotechnol. 55:471-475.
25. Shoham, Y., R. Lamed, and E. A. Bayer. 1999. The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides. Trends Microbiol. 7:275-281.
26. Sun, Y., and J. Cheng. 2002. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour. Technol. 83:1-11.
27. Tajima, M., Y. Nogi, and T. Fukasawa. 1985. Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast 1:67-77.
28. Tomme, P., H. Van Tilbeurgh, G. Pettersson, J. Van Damme, J. Vandekerckhove, J. Knowles, T. Teeri, and M. Claeyssens. 1988. Studies of the cellulolytic system of Trichodema reesei QM9414: analysis of domain function in two cellobiohydrolases by limited proteolysis. Eur. J. Biochem. 170:575-581.
29. Teeri, T. T. 1997. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol. 15:160-167.
30. Teeri, T. T., P. Lehtovaara, S. Kauppinen, I. Salovouri, and J. Knowles. 1987. Homologous domains in Trichoderma reesei cellulolytic enzymes: gene sequence and expression of cellobiohydrolase II. Gene 51:43-52.
31. Van Rensburg, P., W. H. Van Zyl, and I. S. Pretorius. 1996. Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae. Curr. Genet. 30:246-250.
32. Van Rensburg, P., W. H. Van Zyl, and I. S. Pretorius. 1998. Engineering yeast for efficient cellulose degradation. Yeast 14:67-76.
33. Walseth, C. S. 1952. Occurrence of cellulases in enzyme preparations from microorganisms. Tech. Assoc. Pulp Paper Ind. 35:228-233.
34. Wood, B. E., and L. O. Ingram. 1992. Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Appl. Environ. Microbiol. 58:2103-2110.
35. Wood, T. M., and K. M. Bhat. 1988. Methods for measuring cellulase activities. Methods Enzymol. 160:87-112.
36. Woodward, J. 1991. Synergism in cellulose systems. Bioresour. Technol. 36:67-75.
37. Zaldivar, J., J. Nielsen, and L. Olsson. 2001. Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. Appl. Microbiol. Biotechnol. 56:17-34.
38. Zhou, S., and L. O. Ingram. 2001. Simultaneous saccharification and fermentation of amorphous cellulose to ethanol by recombinant Klebsiella oxytoca SZ21 without supplemental cellulase. Biotechnol. Lett. 23:1455-1462.