Enhancement of alkaline phytase production in Pichia pastoris: Influence of gene dosage, sequence optimization and expression temperature

Protein Expression and Purification

Volumn 84, Issue 2, 2012, Pages 247-254

  Yang, Mimi ^a   Johnson, Steven C ^a,b   Murthy, Pushpalatha P N ^a  

^a MICHIGAN TECHNOLOGICAL UNIVERSITY   (United States)

^b UNIVERSITY OF NEW ENGLAND   (United States)

Author keywords

Alkaline phytase; Influence of temperature on heterologous expression; Lily pollen; Phosphate contamination; Phytic acid; Pichia pastoris; Protein expression; qPCR; Transgene dosage; Transgene sequence optimization

Indexed keywords

PHYTASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYMOLOGY; GENE DOSAGE; GENE EXPRESSION; GENETICS; LILY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PLASMID; SEQUENCE ALIGNMENT; TEMPERATURE;

6-PHYTASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GENE DOSAGE; GENE EXPRESSION; LILIUM; MOLECULAR SEQUENCE DATA; PICHIA; PLASMIDS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TEMPERATURE;

ANIMALIA; BOVIDAE; GLYCINE MAX; LILIUM; PICHIA PASTORIS; ZEA MAYS;

EID: 84863536193     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.06.001     Document Type: Article

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