A novel thermostable phytase from the fungus Aspergillus aculeatus RCEF 4894: Gene cloning and expression in Pichia pastoris

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 3, 2011, Pages 679-686

  Ma, Zhong You ^a,b   Pu, Shun Chang ^a   Jiang, Jing Jing ^a   Huang, Bo ^a   Fan, Mei Zhen ^a   Li, Zeng Zhi ^a  

^a ANHUI AGRICULTURAL UNIVERSITY   (China)

^b ANHUI SCIENCE AND TECHNOLOGY UNIVERSITY   (China)

Author keywords

Aspergillus aculeatus; Broad pH optima; High thermostability; Phytase

Indexed keywords

AMINO ACIDS; ASPERGILLUS; CLONE CELLS; GENES; PHOSPHATASES; STABILITY; YEAST;

AMINO ACID SEQUENCE; ASPERGILLUS ACULEATUS; ENZYMATIC ACTIVITIES; GENE CLONING AND EXPRESSIONS; HIGH THERMOSTABILITY; PH OPTIMA; PHYTASES; POLYMERASE CHAIN REACTION TECHNIQUES;

POLYMERASE CHAIN REACTION;

ASPERGILLUS; ASPERGILLUS ACULEATUS; FUNGI; PICHIA PASTORIS;

EID: 79951552973     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-010-0506-4     Document Type: Article

References (29)

1. Arnold K, Bordoli L, Kopp J et al (2006) The SWISS-MODEL workspace: A web-based environment for protein structure homology modelling. Bioinformatics 22: 195-201. doi: 10. 1093/bioinformatics/bti770.
2. Bae HD, Yanke LJ, Cheng KJ et al (1999) A novel staining method for detecting phytase activity. J Microbiol Methods 39: 17-22. doi: 10. 1016/S0167-7012(99)00096-2.
3. Bohn L, Meyer A, Rasmussen S (2008) Phytate: impact on environment and human nutrition. A challenge for molecular breeding. J Zhejiang Univ Sci B 9: 165-191. doi: 10. 1631/jzus. B071064.
4. Chadha BS, Harmeet G, Mandeep M et al (2004) Phytase production by the thermophilic fungus Rhizomucor pusillus. World J Microbiol Biotechnol 20: 105-109. doi: 10. 1023/B: WIBI. 0000013319. 13348. 0a.
5. Cho J, Lee C, Kang S et al (2005) Molecular cloning of a phytase gene (phyM) from Pseudomonas syringae MOK1. Curr Microbiol 51: 11-15. doi: 10. 1007/s00284-005-4482-0.
6. De Preter K, Speleman F, Combaret V et al (2002) Quantification of MYCN, DDX1, and NAG gene copy number in neuroblastoma using a real-time quantitative PCR assay. Mod Pathol 15(2): 159-166. doi: org/10. 1038/modpathol. 3880508.
7. Dvořáková J (1998) Phytase: sources, preparation and exploitation. Folia Microbiol 43: 323-338. doi: 10. 1007/BF02818571.
8. Gargova S, Roshkova Z, Vancheva G (1997) Screening of fungi for phytase production. Biotechnol Tech 11: 221-224. doi: 10. 1023/A: 1018426119073.
9. Guo MJ, Zhuang YP, Chu J et al (2007) Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34. Process Biochem 42: 1660-1665. doi: 10. 1016/j. procbio. 2007. 09. 003.
10. Han Y, Wilson DB, Lei Xg (1999) Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae. Appl Environ Microbiol 65: 1915-1918.
11. Hong CY, Cheng KJ, Tseng TH et al (2004) Production of two highly active bacterial phytases with broad pH optima in germinated transgenic rice seeds. Transgenic Res 13: 29-39. doi: 10. 1023/B: TRAG. 0000017158. 96765. 67.
12. Huang H, Luo H, Wang Y et al (2008) A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions. Appl Microbiol Biotechnol 80: 417-426. doi: 10. 1007/s00253-008-1556-5.
13. Kostrewa D, Gruninger-Leitch F, Darcy A, Broger C, Mitchell D, van Loon APGM (1997) Crystal structure of phytase from Aspergillus ficuum at 2. 5 a resolution. Nat Struct Mol Biol 4(3): 185-190. doi: 10. 1038/nsb0397-185.
14. Lei XG, Porres JM (2003) Phytase enzymology, applications, and biotechnology. Biotechnol Lett 25: 1787-1794. doi: 10. 1023/A: 1026224101580.
15. Lei XG, Porres JM, Mullaney EJ et al (2007) Phytase: source, structure and application. In: Polaina J, MacCabe AP (eds) Industrial enzymes: structure, function and applications. Springer, Dordrecht, pp 505-529. doi: 10. 1007/1-4020-5377-0_29.
16. Liu Q, Huang Q, Lei XG, Hao Q (2004) Crystallographic snapshots of Aspergillus fumigatus phytase, revealing its enzymatic dynamics. Structure 12(9): 1575-1583. doi: 10. 1016/j. str. 2004. 06. 015.
17. Miksch G, Kleist S, Friehs K et al (2002) Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. Appl Microbiol Biotechnol 59: 685-694. doi: 10. 1007/s00253-002-1071-z.
18. Nakashima B, McAllister T, Sharma R et al (2007) Diversity of phytases in the rumen. Microb Ecol 53: 82-88. doi: 10. 1007/s00248-006-9147-4.
19. Pasamontes L, Haiker M, Wyss M et al (1997) Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus. Appl Environ Microbiol 63: 1696-1700.
20. Pavlova K, Gargova S, Hristozova T et al (2008) Phytase from antarctic yeast strain Cryptococcus laurentii AL27. Folia Microbiol 53: 29-34. doi: 10. 1007/s12223-008-0004-3.
21. Selle PH, Ravindran V (2008) Phytate-degrading enzymes in pig nutrition. Livestock Sci 113: 99-122. doi: 10. 1016/j. livsci. 2007. 05. 014.
22. Shi P, Huang H, Wang Y et al (2008) A novel phytase gene appA from Buttiauxella sp. GC21 isolated from grass carp intestine. Aquaculture 275: 70-75. doi: 10. 1016/j. aquaculture. 2008. 01. 021.
23. Shirzadegan M, Christie P, Seemann JR (1991) An efficient method for isolation of RNA from tissue cultured plant cells. Nucl Acids Res 19: 6055.
24. Tseng Y, Fang T, Tseng S (2000) Isolation and characterization of a novel phytase from Penicillium simplicissimum. Folia Microbiol 45: 121-127. doi: 10. 1007/BF02817409.
25. Vohra A, Satyanarayana T (2001) Phytase production by the yeast, Pichia anomala. Biotechnol Lett 23: 551-554. doi: 10. 1023/A: 1010314114053.
26. Wang Y, Gao X, Su Q et al (2007) Cloning, expression, and enzyme characterization of an acid heat-stable phytase from Aspergillus fumigatus WY-2. Curr Microbiol 55: 65-70. doi: 10. 1007/s00284-006-0613-5.
27. Wyss M, Pasamontes L, Remy R et al (1998) Comparison of the thermostability properties of three acid phosphatases from molds: Aspergillus fumigatus phytase, A. niger phytase, and A. niger pH 2. 5 acid phosphatase. Appl Environ Microbiol 64: 4446-4451.
28. Xiang T, Liu Q, Deacon AM, Koshy M, Kriksunov IA, Lei XG, Hao Q, Thiel DJ (2004) Crystal structure of a heat-resilient phytase from Aspergillus fumigatus, carrying a phosphorylated histidine. J Biol Chem 339(2): 437-445. doi: 10. 1016/j. jmb. 2004. 03. 057.
29. Zhang WM, Lei XG (2008) Cumulative improvements of thermostability and pH-activity profile of Aspergillus niger PhyA phytase by site-directed mutagenesis. Appl Microbiol Biotechnol 77: 1033-1040. doi: 10. 1007/s00253-007-1239-7.