Alkaline phytase from Lilium longiflorum: Purification and structural characterization

Protein Expression and Purification

Volumn 46, Issue 2, 2006, Pages 221-232

  Garchow, Barry G ^a   Jog, Sonali P ^a   Mehta, Bakul Dhagat ^a   Monosso, Jodi M ^a   Murthy, Pushpalatha P N ^a  

^a MICHIGAN TECHNOLOGICAL UNIVERSITY   (United States)

Author keywords

Alkaline phytase; Animal feed; Enzyme purification; Ferguson plot; Lilium longiflorum; Phosphate contamination; Pollen grains

Indexed keywords

PHYTASE; VEGETABLE PROTEIN;

ANIMAL FOOD; ARTICLE; CHEMISTRY; DIET SUPPLEMENTATION; ENZYMOLOGY; ISOLATION AND PURIFICATION; LILY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

6-PHYTASE; ANIMAL FEED; CHROMATOGRAPHY, LIQUID; DIETARY SUPPLEMENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; LILIUM; PLANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ANIMALIA; LILIUM; LILIUM LONGIFLORUM;

EID: 33645392484     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.031     Document Type: Article

References (35)

1. R.F. Irvine, and M.J. Schell Back in the water: the return of the inositol phosphates Nat. Rev. Mol. Cell. Biol. 2 2001 327 338
2. F.A. Loewus Structure and occurrence of inositol in plants D.J. Morre W.F. Boss F.A. Loewus Inositol Metabolism in Plants 1990 Wiley-Liss New York, NY 1 11
3. N.R. Reddy, S.K. Sathe, and D.R. Salunkhe Phytates in legumes and cereals Adv. Food Res. 28 1982 1 92
4. T.S. Nelson, T.R. Shieh, R.J. Wodzinski, and J.H. Ware Effect of supplemental phytase on the utilization of phytate phosphorous by chicks J. Nutr. 101 1971 1289 1294
5. X.G. Lei, and C.H. Stahl Nutritional benefits of phytase and dietary determinants of its efficacy J. Appl. Anim. Res. 17 2000 97 112
6. A.N. Sharpley, S.C. Chapra, R. Wedepohl, J.T. Sims, T.C. Daniel, and K.R. Reddy Managing agricultural phosphorous for protection of surface waters: issues and options J. Environ. Qual. 23 1994 437 451
7. R.J. Wodzinski, and A.H.J. Ullah Phytase Adv. Appl. Microbiol. 42 1996 263 302
8. X.G. Lei, and C.H. Stahl Biotechnological development of effective phytases for mineral nutrition and environmental protection Appl. Microbiol. Biotechnol. 57 2001 474 481
9. E.J. Mullaney, and A.H.J. Ullah The term phytase comprises several different classes of enzymes Biochem. Biophys. Res. Commun. 312 2003 179 184
10. U. Konietzny, and R. Greiner Molecular and catalytic properties of phytate-degrading enzymes (phytases) Int. J. Food Sci. Technol. 37 2002 791 812
11. B.-C. Oh, W.-C. Choi, S. Park, Y.-O. Kim, and T.-K. Oh Biochemical properties and substrate specificities of alkaline and histidine acid phytases Appl. Microbiol. Biotechnol. 63 2004 362 372
12. J.J. Scott, and F.A. Loewus A calcium-activated phytase from pollen of Lilium longiflorum Plant Physiol. 82 1986 333 335
13. B.G. Baldi, J.J. Scott, J.D. Everard, and F.A. Loewus Localization of constitutive phytases in lily pollen and properties of the pH 8 form Plant Sci. 56 1988 137 147
14. J.J. Scott Alkaline phytase activity in nonionic detergent extracts of legume seeds Plant Physiol. 95 1991 1298 1301
15. L. Barrientos, J.J. Scott, and P.P.N. Murthy Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen Plant Physiol. 106 1994 1489 1495
16. J. Kerovuo, M. Lauraeus, P. Nurminen, N. Kalkkinen, and J. Apajalahti Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis Appl. Environ. Microbiol. 64 1998 2079 2085
17. J. Kerovuo, J. Rouvinen, and F. Hatzack Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus phytase: indication of a novel reaction mechanism Biochem. J. 352 2000 623 628
18. Y.-O. Kim, J.-K. Lee, H.-K. Kim, J.-H. Yu, and T.-K. Oh Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli FEMS Microbiol. Lett. 162 1998 185 191
19. B.-C. Oh, B.S. Chang, K.-H. Park, N.-C. Ha, H.-K. Kim, B.-H. Oh, and T.-K. Oh Calcium-dependent catalytic activity of a novel phytase from Bacillus amyloliquefaciens DS11 Biochemistry 40 2001 9669 9676
20. D.B. Dickinson Permeability and respiratory properties of germinating pollen Physiol. Plant. 20 1967 118 127
21. F.A. Loewus, and M.W. Loewus Collection and storage of pollen from Lilium longiflorum Plant Cell InCompatibility Newslett. 22 1990 32 36
22. E.F. Rossomando Ion-exchange chromatography Methods Enzymol. 182 1990 309 317
23. L. Giri Chromatofocusing Methods Enzymol. 182 1990 380 392
24. P.H. O'Farrell High resolution two-dimensional electrophoresis of proteins J. Biol. Chem. 250 1975 4007 4021
25. D.S. Ballantyne, and J.R. Warmington Purification of native enolase from medically important Candida species Biotechnol. Appl. Biochem. 31 2000 213 218
26. W.J. Henzel, T.M. Billeci, J.T. Stults, S.C. Wong, C. Grimley, and C. Watanabe Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases Proc. Natl. Acad. Sci. USA 90 1993 5011 5015
27. R. Aebersold, and D.R. Goodlett Mass spectrometry in proteomics Chem. Rev. 101 2001 269 295
28. P. Jungblut, and B. Thiede Protein identification from 2-DE gels by MALDI mass spectrometry Mass Spectrom. Rev. 16 1997 145 162
29. K.M. Bischoff, L. Shi, and P.J. Kennelly The detection of enzyme activity following sodium dodecyl sulfate polyacrylamide gel electrophoresis Anal. Biochem. 260 1998 1 17
30. K. Burridge, and A. Nelson An in-gel assay for protein tyrosine phosphatase activity: detection of widespread distribution in cells and tissues Anal. Biochem. 232 1995 56 64
31. D. Hames One-dimensional polyacrylamide gel electrophoresis B.D. Hames D. Rickwood Gel Electrophoresis of Proteins: A Practical Approach second ed. 1990 Oxford University Press New York, NY 1 147
32. K.A. Ferguson Starch-gel electrophoresis-application to the classification of pituitary proteins and polypeptides Metabolism 13 1964 985 1002
33. J.L. Hedrick, and A.J. Smith Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis Arch. Biochem. Biophys. 126 1968 155 164
34. Sigma, Nondenatured protein molecular weight marker kit, Sigma Chemical Company, St. Louis, MO, 1986, Technical Bulletin No. MKR-137.
35. S.R. Gallagher, One-dimensional electrophoresis using non-denaturing conditions, in: J.E. Coligan, B.M. Dunn, D.W. Speicher, P.T. Wingfield (Eds.), Current Protocols in Protein Science, Wiley, New York, 1995, pp. 10.3.1-10.3.11.