Real-time PCR determination of rRNA gene copy number: Absolute and relative quantification assays with Escherichia coli

Applied Microbiology and Biotechnology

Volumn 78, Issue 2, 2008, Pages 371-376

  Lee, Changsoo ^a   Lee, Seungyong ^b   Shin, Seung Gu ^b   Hwang, Seokhwan ^b  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

Author keywords

Absolute quantification; Escherichia coli; Real time PCR; Relative quantification; rRNA gene copy number

Indexed keywords

BIOASSAY; CHROMOSOMES; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION;

ABSOLUTE QUANTIFICATION; CHROMOSOMAL GENES; REAL TIME POLYMERASE CHAIN REACTION (PCR); RELATIVE QUANTIFICATION; RRNA GENE COPY NUMBER;

RNA;

DNA; RIBOSOME RNA;

BIOASSAY; CHROMOSOME; COLIFORM BACTERIUM; DNA; GENE; POLYMERASE CHAIN REACTION; RNA;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CHROMOSOME; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DEXTRO 1 DEOXYXYLULOSE 5 PHOSPHATE SYNTHASE GENE; ESCHERICHIA COLI; GENE IDENTIFICATION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ASSAY; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

ESCHERICHIA COLI; GENE DOSAGE; GENES, RRNA; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TRANSFERASES;

ESCHERICHIA COLI;

EID: 38649119145     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1300-6     Document Type: Article

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