Muscarinic acetylcholine receptor-mediated activation of Gq in rat brain membranes determined by guanosine-5′-O-(3-[35S]thio) triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay

Journal of Neural Transmission

Volumn 119, Issue 5, 2012, Pages 525-532

  Odagaki, Yuji ^a   Toyoshima, Ryoichi ^a  

^a SAITAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Carbamylcholine chloride (CCh); G proteins; Guanosine 5 O (3 35S thio)triphosphate ( 35S GTP S) binding; Muscarinic acetylcholine receptors (mAChRs); Scintillation proximity assay (SPA)

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANOSINE 5' O (3 [ 35 S]THIO)TRIPHOSPHATE; GUANOSINE DERIVATIVE; GUANOSINE DIPHOSPHATE; MUSCARINIC M1 RECEPTOR; MUSCARINIC RECEPTOR; SNAKE VENOM; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CONCENTRATION (PARAMETERS); CORPUS STRIATUM; HIPPOCAMPUS; IMMUNOASSAY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; RAT; SCINTILLATION PROXIMITY ASSAY; SIGNAL NOISE RATIO;

ANIMALS; BINDING, COMPETITIVE; BRAIN; CELL MEMBRANE; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; MALE; RADIOLIGAND ASSAY; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, MUSCARINIC;

EID: 84863495264     PISSN: 03009564     EISSN: 14351463     Source Type: Journal    
DOI: 10.1007/s00702-011-0742-2     Document Type: Article

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