The [35S]GTPγS binding assay: Approaches and applications in pharmacology

Life Sciences

Volumn 74, Issue 4, 2003, Pages 489-508

  Harrison, C ^a   Traynor, J R ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

G proteins; GTPgammaS; Receptors

Indexed keywords

ADENOSINE A1 RECEPTOR AGONIST; ADENYLATE CYCLASE; ADRENALIN; ALPHA 2 ADRENERGIC RECEPTOR; ANALGESIC AGENT; ANTIASTHMATIC AGENT; ANTIDEPRESSANT AGENT; ANTIHISTAMINIC AGENT; CANNABINOID; CARBACHOL; CARDIOVASCULAR AGENT; CHEMOTACTIC PEPTIDE; FORMYLMETHIONYLLEUCYLPHENYLALANINE; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; LUZINDOLE; MAGNESIUM ION; MUSCARINIC AGENT; NEUROLEPTIC AGENT; OPIATE AGONIST; PHOSPHOLIPASE C; PROTEIN ANTIBODY; PROTEIN SUBUNIT; RGS PROTEIN; SODIUM ION; UNINDEXED DRUG;

ALLOSTERISM; BINDING AFFINITY; BINDING ASSAY; DRUG EFFICACY; DRUG POTENCY; DRUG RECEPTOR BINDING; ERYTHROLEUKEMIA CELL; FEASIBILITY STUDY; GENE EXPRESSION SYSTEM; HUMAN; IMMUNOPRECIPITATION; KIDNEY CELL; NONHUMAN; PHEOCHROMOCYTOMA CELL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RECEPTOR AFFINITY; RECEPTOR BINDING; RECEPTOR DOWN REGULATION; RECEPTOR OCCUPANCY; REVIEW; SIGNAL TRANSDUCTION; STOICHIOMETRY;

EID: 0242552831     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.lfs.2003.07.005     Document Type: Article

References (145)

1. 2C receptors expressed in human embryonic kidney 293 cells. European Journal of Pharmacology. 383:1999;311-319.
2. Alt A., Clark M.J., Woods J.H., Traynor J.R. Mu and delta opioid receptors activate the same G proteins in human neuroblastoma SH-SY5Y cells. British Journal of Pharmacology. 135:2002;217-225.
3. 35S]thio)triphosphate binding in digitonin-permeabilized C6 rat glioma cells: evidence for an organized association of mu-opioid receptors and G protein. Journal of Pharmacology and Experimental Therapeutics. 291:2001;116-121.
4. 35S]thio)triphosphate binding by endogenous opioids acting at a cloned μ receptor. Journal of Pharmacology and experimental Therapeutics. 286:1998;282-288.
5. i/o activation profiles in CHO cells expressing human muscarinic acetylcholine receptors: dependence on agonist as well as receptor-subtype. British Journal of Pharmacology. 132:2001;950-958.
6. 35S]-GTPγS binding. British Journal of Pharmacology. 121:1997;1203-1209.
7. Arunlakshana O., Schild H.O. Some quantitative uses of drug antagonists. British Journal of Pharmacology. 14:1959;48-58.
8. Asano T., Pedersen S.E., Scott C.W., Ross E.M. Reconstitution of catecholamine-stimulated binding of guanosine 5′-O-(3-thiotriphosphate) to the stimulatory GTP-binding protein of adenylate cyclase. Biochemistry. 23:1984;5460-5467.
9. (1D) receptors. Neuropsychopharmacology. 25:2001;410-422.
10. Baker S.P., Scammells P.J., Belardinelli L. Differential A1 adenosine receptor reserve for inhibition of cyclic AMP accumulation and G-protein activation in DDT(1) MF-2 cells. British Journal of Pharmacology. 30:2000;1156-1164.
11. Barr A.J., Brass L.F., Manning D.R. Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells. Journal of Biological Chemistry. 272:1997;2223-2229.
12. 35S]-GTPγS binding. Biochemical Pharmacology. 56:1998;1167-1174.
13. Bernard R., Lydic R., Baghdoyan H.A. Hypocretin-1 activates G proteins in arousal-related brainstem nuclei of rat. Neuroreport. 13:2002;447-450.
14. Birnbaumer L., Abranowitz J., Brown A.M. Receptor-effector coupling by G proteins. Biochimica Biophysica Acta. 1031:1990;163-224.
15. Blumer J.B., Chandler L.J., Lanier S.M. Expression analysis and subcellular distribution of the two G-protein regulators AGS3 and LGN indicate distinct functionality. Journal of Biological Chemistry. 277:2002;15897-15903.
16. Blumer K.J., Thorner J. Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the α subunit but are required for receptor coupling. Proceedings of the National Academy of Science USA. 87:1990;4363-4367.
17. Bokoch G.M., Katada T., Northrup J.K., Ui M., Gilman A.G. Purification and properties of the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Journal of Biological Chemistry. 259:1984;3560-3567.
18. 2: implication for intracellular signalization cross-regulation. Molecular Pharmacology. 55:1999;473-480.
19. Breivogel C.S., Childers S.R. Cannabinoid agonist signal transduction in rat brain: comparison of cannabinoid agonists in receptor binding, G-protein activation, and adenylyl cyclase inhibition. Journal of Pharmacology & Experimental Therapeutics. 295:2000;328-336.
20. Breivogel C.S., Selley D.E., Childers S.R. Acute and chronic effects of opioids on δ and μ receptor activation of G proteins in NG108-15 and SK-N-SH cell membranes. Journal of Neurochemistry. 68:1997;1462-1472.
21. 35S]GTPγS binding to rat cerebellar membranes correlates with agonist-induced decreases in GDP affinity. Journal of Biological Chemistry. 273:1998;16865-16873.
22. q/11 in SH-SY5Y human neuroblastoma cells. British Journal of Pharmacology. 134:2001;257-264.
23. 3); elevated basal signaling activity. Biochemical Journal. 348:2000;531-537.
24. q-linked NK2 receptors mediate neurally induced contraction of human sigmoid circular smooth muscle. Gastroenterology. 119:2000;51-61.
25. Claing A., Laporte S.A., Caron M.G., Lefkowitz R.J. Endocytosis of G protein-coupled receptors: roles of G protein-coupled receptor kinases and beta-arrestin proteins. Progress in Neurobiology. 66:2002;61-79.
26. Costa T., Herz A. Antagonists with negative intrinsic efficacy at δ-opioid receptors coupled to GTP-binding proteins. Proceedings of the National Academy of Science USA. 86:1989;7321-7325.
27. 2C receptors revealed by antibody capture assays: influence of receptor reserve and relationship to agonist-directed trafficking. Molecular Pharmacology. 62:2002;578-589.
28. Dallas-Yang Q., Qureshi S.A., Xie D., Zhang B.B., Jiang G. Detection of glucagon-dependent GTPγS binding in high-throughput format. Analalytical Biochemistry. 301:2002;156-159.
29. 35S]guanosine-5′-O- (3-thio)triphosphate binding mediated by cholinergic muscarinic receptors in membranes from Chinese hamster ovary cells and rat striatum using an anti-G protein scintillation proximity assay. Journal of Pharmacology and Experimental Therapeutics. 289:1999;946-955.
30. De Vries L., Zheng B., Fischer T., Elenko E., Farquhar M.G. The regulator of G protein signaling family. Annual Review of Pharmacology and Toxicology. 40:2000;235-271.
31. 351 in the rat Gαo protein. Neuropharmacology. 38:1999;1035-1041.
32. 35S]-thio)triphosphate binding. British Journal of Pharmacology. 121:1997;1422-1428.
33. Emmerson P.J., Clark M.J., Mansour A., Akil H., Woods J.H., Medzihradsky F. Characterization of opioid agonist efficacy in a C6 glioma cell line expressing the μ opioid receptor. Journal of Pharmacology and Experimental Therapeutics. 278:1996;1121-1127.
34. 2 vasopressin receptor agonist- and regulator of G-protein-signaling-sensitive G protein. Analytical Biochemistry. 300:2002;212-220.
35. 35S]guanosine-5′-O-(3-thio)triphosphate binding. Molecular Pharmacology. 54:1998;249-257.
36. 5A receptor couples to Gi/Go proteins and inhibits adenylate cyclase in HEK 293 cells. European Journal of Pharmacology. 361:1998;299-309.
37. Fung B.K. Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits. Journal of Biological Chemistry. 258:1983;10495-10502.
38. Furchgott R.F. The use of β-haloalkylamines in the differentiation of receptors and the determination of dissociation constants of receptor-agonist complexes. Harper N.J., Simmonds A.B. Advances in Drug Research. vol 3:1966;21-25 Academic Press, New York.
39. B R1 subunit. Molecular Pharmacology. 57:2000;419-426.
40. 35S]GTPγS binding correlates with Alzheimer's disease neurofibrillary pathology in entorhinal cortex and CA1 hippocampal subfield. Journal of Neuroscience Research. 67:2002;388-398.
41. 4 receptor. Naunyn-Schmiedebergs Archives of Pharmacology. 36:2000;5555-5564.
42. Gierschik P., Moghtader R., Straub C., Dieterich K., Jakobs K.H. Signal amplification in HL-60 granulocytes. European journal of Biochemistry. 197:1991;725-732.
43. 35S] GTPγS binding assays. Naunyn Schmiedeberg's Archives of Pharmacology. 361:2000;498-504.
44. Gonzalez-Maeso J., Torre I., Rodriguez-Puertas R., Garcia-Sevilla J.A., Guimon J., Meana J.J. Effects of age, postmortem delay and storage time on receptor-mediated activation of G-proteins in human brain. Neuropsychopharmacology. 26:2002;468-478.
45. Gou Z.-D., Suga H., Okamura M., Takeda S., Haga T. Receptor-Gα fusion proteins as a tool for ligand screening. Life Sciences. 68:2001;2319-2327.
46. i1 protein subunits promotes high-affinity agonist binding to the neurotensin receptor NTS-1 expressed in Escherichia coli. FEBS Letters. 493:2001;101-105.
47. 2c receptor antagonists. Journal of Biological Chemistry. 271:1996;22591-22597.
48. Hashiba E., Lambert D.G., Jenck F., Wicjmann J., Smith G. Characterization of the non-peptide nociceptin receptor agonist Ro64-6198 in Chinese hamster ovary cells expressing recombinant human nociceptin receptors. Life Sciences. 70:2002;1719-1725.
49. 35S]thio) triphosphate to guanine nucleotide binding proteins in cardiac membranes. European Journal of Biochemistry. 186:1989;725-731.
50. Hilf G., Kupprion C., Wieland T., Jakobs K.H. Dissociation of guanosine 5′-[γ-thio] triphosphate from guanine-nucleotide-binding regulatory proteins in native cardiac membranes. European Journal of Biochemistry. 204:1992;725-731.
51. Hong W., Werling L. Lack of effects by sigma ligands on neuropeptide Y-induced G-protein activation in rat hippocampus and cerebellum. Brain Research. 901:2001;208-218.
52. Horstman D.A., Brandon S., Wilson A.L., Guyer C.A., Cragoe E.J. Jr., Limbird L.E. An aspartate conserved among G-protein receptors confers allosteric regulation of alpha 2-adrenergic receptors by sodium. Journal of Biological Chemistry. 265:1990;21590-21595.
53. q-protein coupling. Journal of Neurochemistry. 80:2002;678-696.
54. Iňiguez-Lluhi J., Simon M.I., Robishaw J.D., Gilman A.G. G protein βγ subunits synthesized in Sf9 cells. Functional characterization and the significance of prenylation of γ Journal of Biological Chemistry. 267:1992;23409-23417.
55. 35S]thiotriphosphate binding to liposomes with varying muscarinic receptor/Go protein stoichiometry. Molecular Pharmacology. 1954:1998;899-906.
56. Jian X., Sainz E., Clark W.A., Jensen R.T., Battey J.F., Northup J.K. The bombesin receptor subtypes have distinct G protein specificities. Journal of Biological Chemistry. 274:1999;11573-11581.
57. 1 dopamine receptors couple to multiple Gα proteins in different brain regions. Journal of Neurochemistry. 78:2001;981-990.
58. i1α. Molecular Pharmacology. 56:1999;684-692.
59. Kenakin T. Drug efficacy at G protein-coupled receptors. Annual Review of Pharmacology and Toxicology. 42:2002;349-379.
60. 35S]GTPγS binding assessment of metabotropic glutamate receptor standards in Chinese hamster ovary cell lines expressing the human metabotropic receptor subtypes 2 and 4. Neuropharmacology. 37:1998;179-187.
61. s. Naunyn Schmiedebergs Archives of Pharmacology. 365:2002;231-241.
62. Kurose H., Katada T., Haga T., Haga K., Ichiyama A., Ui M. Functional interaction of purified muscarinic receptors with purified inhibitory guanine nucleotide regulatory proteins reconstituted in phospholipid vesicles. Journal of Biological Chemistry. 261:1986;6423-6428.
63. 1 receptor binding and activation of GTP-binding proteins in the basal ganglia of patients with Parkinson's syndrome and of MPTP-treated marmosets. European Journal of Neuroscience. 14:2001;1827-1832.
64. 35S]GTPγS binding is a tool for pharmacological characterization of G protein-coupled dinucleotide receptors. Life Sciences. 65:1999;PL183-PL190.
65. 35S] GTPγS binding to cell membranes. Methods in Molecular Biology. 106:1999;231-245.
66. Lazareno S., Farries T., Birdsall N.J.M. Pharmacological characterization of guanine nucleotide exchange reactions in membranes from CHO cells stably transfected with human muscarinic receptors m1-m4. Life Sciences. 52:1993;449-456.
67. Lefkowitz R.J., Cotecchia S., Samama P., Costa T. Constitutive activity of receptors coupled to guanine nucleotide regulatory proteins. Trends in Pharmacological Sciences. 14:1993;203-207.
68. 2A-adrenoceptor: allosteric modulation by amilorides. Molecular Pharmacology. 58:2000;1091-1099.
69. Linder M.E., Middleton P., Hepler J.R., Taussig R., Gilman A.G., Mumby S.M. Lipid modifications of G proteins: α subunits are palmitoylated. Proceedings of the National Acadamy of Sciences USA. 90:1993;3675-3679.
70. Liu J.G., Liao X.P., Gong Z.H., Qin B.Y. Methadone-induced desensitization of the delta-opioid receptor is mediated by uncoupling of receptor from G protein. European Journal of Pharmacology. 374:1999;301-308.
71. 5]enkephalin. Journal of Biological Chemistry. 41:2001;37779-37786.
72. Lorenzen A., Beukers M.W., van de Graff P.H., Lang H., van Muijlwijk-Koezen J., de Groote M., Menge W., Schwabe U., Ijzerman A.P. Modulation of agonist responses at the A1 adenosine receptor by an irreversible antagonist, receptor-G protein uncoupling and by G protein activation state. Biochemical Pharmacology. 64:2002;1251-1265.
73. 35S]-thio)-triphosphate binding. Molecular Pharmacology. 44:1993;2115-2123.
74. Lorenzen A., Guerra L., Schwabe U. Interaction of full and partial agonists of the A1 adenosine receptor with receptor/G protein complexes in rat brain membranes. Molecular Pharmacology. 49:1996;915-926.
75. Mandala S., Hajdu R., Bergstrom J., Quackenbush E., Xie J., Milligan J., Thornton R., Shei G.J., Card D., Keohane C., Rosenbach M., Hale J., Lynch C.L., Rupprecht K., Parsons W., Rosen H. Alteration of lymphocyte trafficking by sphingosine-1-phosphate receptor agonists. Science. 296:2002;346-349.
76. 16 Fusion Product. J Biomol Screen. 7:2002;281-289.
77. i2α fused to the human mu opioid receptor differently. Journal of Neurochemistry. 81:2002;1372-1382.
78. 1 coexpression and fusion protein studies. Molecular Pharmacology. 58:2000;407-412.
79. 1A receptors. Journal of Neurochemistry. 74:2000;347-357.
80. 35S]GTPγS binding to G-proteins in postmortem brains of opioid addicts. Molecular Psychiatry. 5:2000;308-315.
81. Milligan G. Insights into ligand pharmacology using receptor-G-protein fusion proteins. Trends in Pharmacological Sciences. 21:2000;24-28.
82. Miyamoto A., Laufs U., Pardo C., Liao J.K. Modulation of bradykinin receptor ligand binding affinity and its coupled G-proteins by nitric oxide. Journal of Biological Chemistry. 272:1997;19601-19608.
83. Mueller A., Mahmoud N.G., Goedecke M.C., McKeating J.A., Strange P.G. Pharmacological characterization of the chemokine receptor, CCR5. British Journal of Pharmacology. 135:2002;1033-1043.
84. 2-adrenoceptors. Biochemical Pharmacology. 62:2001;913-922.
85. Neilan C.L., Akil H., Woods J.H., Traynor J.R. Constitutive activity of the delta-opioid receptor expressed in C6 glioma cells: identification of non-peptide delta-inverse agonists. British Journal of Pharmacology. 128:1999;556-562.
86. 1D receptors. Naunyn-Schmiedebergs Archives of Pharmacology. 359:1999;447-453.
87. 35S]thio)- triphosphate binding and antibody study. Molecular Pharmacology. 55:1999;564-574.
88. 1A receptors as a function of receptor: G-protein stoichiometry. Neuropharmacology. 36:1997;451-459.
89. 1A receptor-mediated Gαi3 activation; conformational selection by high-efficacy agonists, and relationship to trafficking of receptor signaling. Molecular Pharmacology. 62:2002;590-601.
90. 2 melatonin receptors: evidence for agonist activity of some mt1-antagonists. British Journal of Pharmacology. 127:1999;1288-1294.
91. Ohtaki T., Kumano S., Ishibashi Y., Ogi K., Matsui H., Harada M., Kitada C., Kurokaw T., Onda H., Fujino M. Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus. Journal of Biological Chemistry. 274:1999;37041-37045.
92. Ozaki S., Kawamoto H., Itoh Y., Miyaji M., Azuma T., Ichikawa D., Nambu H., Iguchi T., Iwasawa Y., Ohta H. In vitro and in vivo pharmacological characterization of J-113397, a potent and selective non-peptidyl ORL1 receptor antagonist. European Journal of Pharmacology. 18(402):2000;45-53.
93. 1-like receptor agonists. Neurochemical Research. 25:2000;759-767.
94. 2A adrenoceptors by ligands with agonist, silent and inverse agonist properties. Naunyn Schmiedebergs Archives of Pharmacology. 366:2002;134-141.
95. Pemberton A.D., Zamolodchikova T.S., Scudamore C.L., Chilvers E.R., Miller H.R., Walker T.R. Proteolytic action of duodenase is required to induce DNA synthesis in pulmonary artery fibroblasts. European Journal of Biochemistry. 269:2002;1171-1180.
96. Pitcher J.A., Inglese J., Higgins J.B., Arriza J.L., Casey P.J., Kim C., Benovic J.L., Kwatra M.M., Caron M.G., Lefkowitz R.J. Role of βγ subunits of G proteins in targeting the β-adrenergic receptor kinase to membrane-bound receptors. Science. 257:1992;1264-1267.
97. q -coupled receptors is mediated by Gβγ exchange. Proceedings of the National Academy of Sciences of the United States of America. 96:1999;10626-10631.
98. Rane M.J., Prossnitz E.R., Arthur J.M., Ward R.A., McLeish K.R. Deficient homologous desensitization of formyl peptide receptors stably expressed in undifferentiated HL-60 cells. Biochemical Pharmacology. 60:2000;179-187.
99. Remmers A.E., Clark M.J., Alt A., Medzihradsky F., Woods J.H., Traynor J.R. Activation of G protein by opioid receptors: role of receptor number and G protein concentration. European Journal of Pharmacology. 396:2000;67-75.
100. Rezaei K., Saar K., Soomets U., Valkna A., Nasman J., Zorko M., Akerman K., Schroeder T., Bartfai T., Langel U. Role of third intracellular loop of galanin receptor type 1 in signal transduction. Neuropeptides. 34:2000;25-31.
101. Ross E.M., Wilkie T.M. GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins. Annual Review of Biochemistry. 69:2000;795-827.
102. 3 receptors. British Journal of Pharmacology. 135:2002;383-392.
103. Selley D.E., Cao C.C., Liu Q., Childers S.R. Effects of sodium on agonist efficacy for G-protein activation in μ-opioid receptor-transfected CHO cells and rat thalamus. British Journal of Pharmacology. 130:2000;987-996.
104. 35S]GTPγS binding in mMOR-CHO cells and rat thalamus. Journal of Pharmacology and Experimental Therapeutics. 285:1998;496-505.
105. Selley D.E., Sim L.J., Xiao R., Liu Q., Childers S.R. μ-opioid receptor-stimulated guanosine-5′-O-(γ-thio)triphosphate binding in rat thalamus and cultured cell lines: signal transduction mechanisms underlying agonist efficacy. Molecular Pharmacology. 51:1997;87-96.
106. Serradeil-Le Gal C., Lafontan M., Raufaste D., Marchand J., Pouzet B., Casellas P., Pascal M., Maffrand J.P., Le Fur G. Characterization of NPY receptors controlling lipolysis and leptin secretion in human adipocytes. FEBS Letters. 475:2000;150-156.
107. 35S] GTPγS binding in Hcrtr2-transfected cell lines and in brain homogenate. Biochemical and Biophysical Research Communications. 294:2002;615-620.
108. Shirai H., Takahashi K., Katada T., Inagami T. Mapping of G protein coupling sites of the angiotensin II type 1 receptor. Hypertension. 25:1995;726-730.
109. Siehler S., Hoyer D. Characterization of human recombinant somatostatin receptors. 3. Modulation of adenylate cyclase activity. Naunyn-Schmiedeberg's Archives of Pharmacology. 360:1999;510-521.
110. 35S]guanyl-5′-O- (γ-thio)-triphosphate binding in guinea-pig brain. Journal of Comparative Neurology. 386:1997;562-572.
111. 35S]GTPγS autoradiography in rat brain. Journal of Neuroscience. 16:1996;2684-2692.
112. 35S]thio]-triphosphate binding. Proceedings of the National Academy of Sciences USA. 92:1995;7242-7246.
113. 35S]GTPγS binding. Methods in Molecular Biology. 83:1997;117-132.
114. 35S]GTPγS binding in rat brain. Neuroreport. 7:1996;729-733.
115. 35S]guanyl-5′-O-(γ-thio)-triphosphate binding in cynomolgus monkey brain. Neuroscience. 94:1999;651-662.
116. Sim-Selley L.J., Selley D.E., Vogt L.J., Childers S.R., Martin T.J. Chronic heroin self-administration desensitizes μ opioid receptor-activated G-proteins in specific regions of rat brain. Journal of Neuroscience. 2:2000;4555-4562.
117. Simon M.I., Strathman M.P., Gautam N. Diversity of G proteins in Signal Transduction. Science. 252:1991;802-808.
118. 35S]GTPγS. Brain Research Brain Research Reviews. 38:2001;149-164.
119. Stanasila L., Lim W.K., Neubig R.R., Pattus F. Coupling efficacy and selectivity of the human μ-opioid receptor expressed as receptor-Gα fusion proteins in Escherichia coli. Journal of Neurochemistry. 75:2000;1190-1199.
120. Sternweis P.C., Robishaw J.D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. Journal of Biological Chemistry. 259:1984;13806-13813.
121. Strange P.G. Mechanisms of inverse agonism at G-protein-coupled receptors. Trends in Pharmacological Sciences. 23:2002;89-95.
122. 35S]thio)triphosphate to NG108-15 cell membranes: characterization of agonist and inverse agonist effects. Journal of Pharmacology and Experimental Therapeutics. 283:1997;1276-1284.
123. 2 and receptor differentiation: a statistical analysis of competitive antagonism. Life Sciences. 25:1979;637-654.
124. 2D-adrenergic receptor regulated G protein activation in PC12 cell membranes. Pharmacology. 52:1996;252-262.
125. 2-adrenergic receptor activation of G proteins: evidence for a precoupled receptor/G protein state. Molecular Pharmacology. 45:1994;524-531.
126. 35S]thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Molecular pharmacology. 47:1995;848-854.
127. 2 subtypes is caused by their sequestration/internalization. Journal of Biochemistry (Tokyo). 124:1998;863-868.
128. 35S]GTPγS and cAMP functional assays. European Journal of Pharmacology. 411:2000;211-221.
129. 3 receptors. Journal of Medicinal Chemistry. 44:2001;2966-2975.
130. 1b adrenoreceptors in Rat-1 fibroblasts. Journal of Biological Chemistry. 272:1997;27330-27337.
131. i1α fusion protein expressed in Sf9 insect cells. Acta Pharmacology Sin. 23:2002;230-236.
132. 5 muscarinic receptors expressed in CHO-K1 cells. British Journal of Pharmacology. 127:1999;590-596.
133. 3. Journal of Biological Chemistry. 274:1999;33259-33266.
134. 2-adrenergic coupling to Gs-, Gi-, and Gq-proteins. Molecular Pharmacology. 58:2000;954-966.
135. Wieland T., Liedel K., Kaldenberg-Stasch S., Meyer zu Heringdorf D., Schmidt M., Jakobs K.H. Analysis of receptor-G protein interactions in permeabilized cells. Naunyn-Schmiedeberg's Archives of Pharmacology. 351:1995;329-336.
136. Wieland T., Kreiss J., Gierschik P., Jakobs K.H. Role of GDP in formyl-peptide-receptor-induced activation of guanine-nucleotide-binding proteins in membranes of HL60 cells. European Journal of Biochemistry. 205:1992;1201-1206.
137. 35S]guanosine-5′-O-(3-thio)triphosphate binding: agonist potencies and the influence of sodium chloride on intrinsic activity. Molecular Pharmacology. 51:1997;1060-1069.
138. 1 adenosine receptor-Gi/o alpha fusion proteins. Biochemistry. 38:1999;2272-2278.
139. Xiao Z.-L., Chen Q., Amaral J., Biancani P., Behar J. Defect of receptor-G protein coupling in human gall bladder with cholesterol stones. American Journal of Physiology. Gastrointestinal and Liver Physiology. 278:2000;G251-G258.
140. Yang Q., Lanier S.M. Influence of G protein type on agonist efficacy. Molecular Pharmacology. 56:1999;651-656.
141. Zahn K., Eckstein N., Trankle C., Sadee W., Mohr K. Allosteric modulation of muscarinic receptor signaling: alcuronium-induced conversion of pilocarpine from an agonist into an antagonist. Journal of Pharmacology and Experimental Therapeutics. 301:2002;720-728.
142. Zaki P., Keith D.E., Thomas J.B., Carroll F.I., Evans C.J. Agonist- and antagonist- and inverse agonist-regulated trafficking of the δ-opioid receptor correlates with, but does not require G protein activation. Journal of Pharmacology and Experimental Therapeutics. 298:2001;1015-1020.
143. Zarbin M.A., Palacios J.M., Wamsley J.K., Kuhar M.J. Axonal transport of beta-adrenergic receptors. Antero- and retrogradely transported receptors differ in agonist affinity and nucleotide sensitivity. Molecular Pharmacology. 24:1983;341-348.
144. 5 functionally couples to inhibitory G proteins and undergoes desensitization. Journal of Cellular Biochemistry. 71:1998;36-45.
145. 35S]GTPγS binding to membranes: determination of potencies and efficacies of ligands. Journal of Pharmacology and Experimental Therapeutics. 282:1997;676-684.