A simplified reconstitution of mRNA-directed peptide synthesis: Activity of the epsilon enhancer and an unnatural amino acid

Analytical Biochemistry

Volumn 297, Issue 1, 2001, Pages 60-70

  Forster, Anthony C ^a   Weissbach, Herbert ^b   Blacklow, Stephen C ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b FLORIDA ATLANTIC UNIVERSITY   (United States)

Author keywords

Biotin; E. coli; Elongation factor; Enhancer; Epsilon; Initiation factor; Peptide; Reconstitution; Ribosome; Translation; TRNA; Unnatural amino acid

Indexed keywords

AMINO ACIDS; ELONGATION; ESCHERICHIA COLI; MACROMOLECULES; PEPTIDES; PURIFICATION; TRANSLATION (LANGUAGES);

BIOTIN; E. COLI; ELONGATION FACTOR; ENHANCER; EPSILON; INITIATION FACTORS; RECONSTITUTION; RIBOSOME; TRNA; UNNATURAL AMINO ACIDS;

BIOSYNTHESIS;

AMINO ACID; DIPEPTIDE; ELONGATION FACTOR; INITIATION FACTOR; MESSENGER RNA; OLIGOPEPTIDE; TRIPEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC RECOMBINATION; MOLECULAR CLONING; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; RIBOSOME; RNA TRANSLATION;

EID: 0035477877     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5329     Document Type: Article

References (63)

1. Inhibitors of translation
2. Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors
3. Late events of translation initiation in bacteria: A kinetic analysis
4. Mimicry grasps reality in translation termination
5. Translation initiation: Adept at adapting
6. DNA-directed in vitro synthesis of beta-galactosidase: Studies with purified factors
7. The mRNA-directed synthesis of the alpha-peptide of beta-galactosidase, ribosomal proteins L12 and L10, and elongation factor Tu, using purified translational factors
8. Requirements for in vitro reconstruction of protein synthesis
9. Isolation and point of action of a factor from Escherichia coli required to reconstruct translation
10. The joining of ribosomal subunits in eukaryotes requires eIF5B
11. Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond
12. In vitro expression of Escherichia coli ribosomal protein L 10 gene: Tripeptide synthesis as a measure of functional mRNA
13. Translation initiation factor IF1 is essential for cell viability in Escherichia coli
14. Altered translation initiation factor 2 in the cold-sensitive ssyG mutant affects protein export in Escherichia coli
15. Escherichia coli protein synthesis initiation factor IF3 controls its own gene expression at the translational level in vivo
16. Prokaryotic and eukaryotic translation factors
17. Both forms of translational initiation factor IF2 (alpha and beta) are required for maximal growth of Escherichia coli: Evidence for two translational initiation codons for IF2 beta
18. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli
19. The downstream box: An efficient and independent translation initiation signal in Escherichia coli
20. Chemical synthesis and in vivo hyperexpression of a modular gene coding for Escherichia coli translational initiation factor IF1
21. A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli
22. Regulated expression of foreign genes fused to lac: Control by glucose levels in growth medium
23. The role of cysteinyl residues in the activity of bacterial elongation factor Ts, a guanosine nucleotide dissociation protein
24. The "allosteric three-site model" of elongation cannot be confirmed in a well-defined ribosome system from Escherichia coli
25. Purification and characterization of protein synthesis initiation factors IF1, IF2, and IF3 from Escherichia coli
26. Superexpression and fast purification of E coli initiation factor IF2
27. Studies on the in vitro synthesis of beta-galactosidase: Necessary components in the ribosomal wash
28. Synthesis of small RNAs using T7 RNA polymerase
29. Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites
30. Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase
31. In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor
32. Nucleoside triphosphate regeneration decreases the frequency of translation errors
33. In vitro selection and evolution of functional proteins by using ribosome display
34. The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli
35. Discrimination by Escherichia coli initiation factor IF3 against initiation on non-canonical codons relies on complementarity rules
36. Characterization of the ribosome-dependent guanosine triphosphatase activity of polypeptide chain initiation factor IF 2
37. Relationship between size of mRNA ribosomal binding site and initiation factor function
38. Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs
39. Mapping the path of the nascent peptide chain through the 23S RNA in the 50S ribosomal subunit
40. GTP consumption of elongation factor Tu during translation of heteropolymeric mRNAs
41. Fast recycling of Escherichia coli ribosomes requires both ribosome recycling factor (RRF) and release factor RF3
42. A simplified in vitro system to measure gene expression based on dipeptide synthesis
43. Initiation factors IF1 and IF2 synergistically remove peptidyl-tRNAs with short polypeptides from the P-site of translating Escherichia coli ribosomes
44. Recycling of initiation factors IF-1, IF-2 and IF-3
45. An in vitro polysome display system for identifying ligands from very large peptide libraries
46. Factor-free ("non-enzymic") and factor-dependent systems of translation of polyuridylic acid by Escherichia coli ribosomes
47. Molecular movement inside the translational engine
48. High-level translation initiation
49. Crystal structure of an initiation factor bound to the 30S ribosomal subunit
50. In vitro codon-reading specificities of unmodified tRNA molecules with different anticodons on the sequence background of Escherichia coli tRNASer
51. Codon reading properties of an unmodified transfer RNA
52. Transfer RNA modification: Influence on translational frameshifting and metabolism
53. Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA
54. Footprinting mRNA-ribosome complexes with chemical probes
55. The gene encoding the elongation factor P protein is essential for viability and is required for protein synthesis
56. Molecular characterization of a prokaryotic translation factor homologous to the eukaryotic initiation factor eIF4A
57. Co-translational folding
58. Processing of the N termini of nascent polypeptide chains requires deformylation prior to methionine removal
59. Degradation of mRNA in Escherichia coli: An old problem with some new twists
60. A general method for site-specific incorporation of unnatural amino acids into proteins
61. In vitro virus: Bonding of mRNA bearing puromycin at the 3′-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro
62. RNA-peptide fusions for the in vitro selection of peptides and proteins