ECSTASY, an adjustable membrane-tethered/soluble protein expression system for the directed evolution of mammalian proteins

Protein Engineering, Design and Selection

Volumn 25, Issue 7, 2012, Pages 367-375

  Chen, Cheng Pao ^a   Hsieh, Yuan Ting ^a,b   Prijovich, Zeljko M ^a   Chuang, Huai Yao ^a,c   Chen, Kai Chuan ^a   Lu, Wei Cheng ^a   Tseng, Qingzong ^a   Leu, Yu Lin ^d   Cheng, Tian Lu ^e   Roffler, Steve R ^a  

^a INSTITUTE OF BIOMEDICAL SCIENCES   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

^c NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

^d CHIA NAN UNIVERSITY OF PHARMACY AND SCIENCE   (Taiwan)

^e KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

Author keywords

glucuronidase; directed evolution; GPI anchor; high throughput screening; mammalian cell surface display

Indexed keywords

ACTIVE PROTEINS; ACTIVITY-BASED; ANTIBODY-DIRECTED ENZYME PRODRUG THERAPIES; CAMPTOTHECIN (CPT); CANCER THERAPY; CULTURE PLATES; DIRECTED EVOLUTION; FLUORESCENCE ACTIVATED CELL SORTING; GLUCURONIDASE; GLYCOSYL; HIGH-THROUGHPUT; HIGH-THROUGHPUT SCREENING; MAMMALIAN CELLS; MAMMALIAN PROTEINS; MEMBRANE-TETHERED PROTEINS; MULTIPLEX MEASUREMENT; PHOSPHATIDYLINOSITOL; PHOSPHOLIPASE C; PRODRUGS; PROTEIN CONCENTRATIONS; PROTEIN EXPRESSION SYSTEMS; PROTEIN VARIANTS; RETROVIRAL TRANSDUCTION; SCREENING SYSTEM; SOLUBLE PROTEINS; SURFACE TETHERED; WILD TYPES;

ANIMAL CELL CULTURE; CELL MEMBRANES; CHEMOTHERAPY; CLONE CELLS; FLUORESCENCE; MAMMALS; RECOMBINANT PROTEINS;

PLANTS (BOTANY);

BETA GLUCURONIDASE; FIRTECAN; MEMBRANE PROTEIN; PHOSPHOLIPASE C; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANTIBODY DIRECTED ENZYME PRODRUG THERAPY; ARTICLE; BLADDER CANCER; CANCER CELL CULTURE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME CLEAVABLE SURFACE TETHERED ALL PURPOSE SCREENING SYSTEM; ENZYME STABILITY; FIBROBLAST; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION SYSTEM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MAMMAL CELL; MICROTITER PLATE ASSAY; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN HYDROLYSIS; PROTEIN SECRETION; RETROVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION; WILD TYPE;

3T3 CELLS; ANIMALS; CELL LINE; GENE EXPRESSION; GLUCURONIDASE; GPI-LINKED PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICE; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SOLUBILITY;

MAMMALIA;

EID: 84863002974     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs033     Document Type: Article

References (33)

1. Apweiler, R., Hermjakob, H. and Sharon, N. (1999) Biochim. Biophys. Acta, 1473, 4-8.
2. Brewis, I.A., Turner, A.J. and Hooper, N.M. (1994) Biochem. J., 303(Part 2), 633-638.
3. Chen, K.C., Cheng, T.L., Leu, Y.L., Prijovich, Z.M., Chuang, C.H., Chen, B.M. and Roffler, S.R. (2007) Cancer Gene Ther., 14, 187-200.
4. Chen, K.C., Wu, C.H., Chang, C.Y., et al. (2008) Chem. Biol., 15, 1277-1286.
5. Chen, K.C., Wu, S.Y., Leu, Y.L., Prijovich, Z.M., Chen, B.M., Wang, H.E., Cheng, T.L. and Roffler, S.R. (2011) Bioconjug. Chem., 22, 938-948.
6. Cheng, T.L., Liao, K.W., Tzou, S.C., Cheng, C.M., Chen, B.M. and Roffler, S.R. (2004) Cancer Gene Ther., 11, 380-388.
7. Chou, W.C., Liao, K.W., Lo, Y.C., Jiang, S.Y., Yeh, M.Y. and Roffler, S.R. (1999) Biotechnol. Bioeng., 65, 160-169.
8. de Graaf, M., Pinedo, H.M., Oosterhoff, D., van der Meulen-Muileman, I.H., Gerritsen, W.R., Haisma, H.J. and Boven, E. (2004) Hum. Gene Ther., 15, 229-238.
9. Durocher, Y. and Butler, M. (2009) Curr. Opin. Biotech., 20, 700-707.
10. Fernandez-Gacio, A., Uguen, M. and Fastrez, J. (2003) Trends Biotechnol., 21, 408-414.
11. Fox, R.J., Davis, S.C., Mundorff, E.C., et al. (2007) Nat. Biotechnol., 25, 338-344.
12. Garcia-Martin, M.L., Herigault, G., Remy, C., Farion, R., Ballesteros, P., Coles, J.A., Cerdan, S. and Ziegler, A. (2001) Cancer Res., 61, 6524-6531.
13. Helmlinger, G., Yuan, F., Dellian, M. and Jain, R.K. (1997) Nat. Med., 3, 177-182.
14. Huang, P.T., Chen, K.C., Prijovich, Z.M., Cheng, T.L., Leu, Y.L. and Roffler, S.R. (2011) Cancer Gene Ther., 18, 381-389.
15. Jain, S., Drendel, W.B., Chen, Z.W., Mathews, F.S., Sly, W.S. and Grubb, J.H. (1996) Nat. Struct. Biol., 3, 375-381.
16. Kukuruzinska, M.A., Bergh, M.L. and Jackson, B.J. (1987) Annu. Rev. Biochem., 56, 915-944.
17. Liao, K.W., Lo, Y.C. and Roffler, S.R. (2000) Gene Ther., 7, 339-347.
18. Lipovsek, D., Antipov, E., Armstrong, K.A., Olsen, M.J., Klibanov, A.M., Tidor, B. and Wittrup, K.D. (2007) Chem. Biol., 14, 1176-1185.
19. Marshall, C., Franks, L. and Carbonell, A. (1977) J. Natl Cancer I., 58, 1743.
20. Martin, G.R. and Jain, R.K. (1994) Cancer Res., 54, 5670-5674.
21. Mastrobattista, E., Taly, V., Chanudet, E., Treacy, P., Kelly, B.T. and Griffiths, A.D. (2005) Chem. Biol., 12, 1291-1300.
22. Okui, N. and Okada, E. (2005) J. Biomed. Opt., 10, 1-8.
23. Prijovich, Z.M., Chen, K.C. and Roffler, S.R. (2009) Mol. Cancer Ther., 8, 940-946.
24. Shipley, J.M., Grubb, J.H. and Sly, W.S. (1993) J. Biol. Chem., 268, 12193-12198.
25. Sitia, R. and Braakman, I. (2003) Nature, 426, 891-894.
26. Stemmer, W.P., Crameri, A., Ha, K.D., Brennan, T.M. and Heyneker, H.L. (1995) Gene, 164, 49-53.
27. Tu, R., Martinez, R., Prodanovic, R., Klein, M. and Schwaneberg, U. (2011) J. Biomol. Screen., 16, 285-294.
28. Turner, N.J. (2009) Nat. Chem. Biol., 5, 567-573.
29. Varadarajan, N., Cantor, J.R., Georgiou, G. and Iverson, B.L. (2009) Nat. Protoc., 4, 893-901.
30. Wolfe, L.A., Mullin, R.J., Laethem, R., Blumenkopf, T.A., Cory, M., Miller, J.F., Keith, B.R., Humphreys, J. and Smith, G.K. (1999) Bioconjugate Chem., 10, 38-48.
31. Wu, C.H., Balasubramanian, W.R., Ko, Y.P., Hsu, G., Chang, S.E., Prijovich, Z.M., Chen, K.C. and Roffler, S.R. (2004) Biotechnol. Appl. Biochem., 40, 167-172.
32. Yoo, T.H., Pogson, M., Iverson, B.L. and Georgiou, G. (2012) Chembiochem., 13, 649-653.
33. Yu, Y.Y.L., Netuschil, N., Lybarger, L., Connolly, J.M. and Hansen, T.H. (2002) J. Immunol., 168, 3145.