A simple method for the production of recombinant proteins from mammalian cells

Biotechnology and Applied Biochemistry

Volumn 40, Issue 2, 2004, Pages 167-172

  Wu, Chia Hung ^a   Balasubramanian, Wesley Roy ^a   Ko, Ya Ping ^a   Hsu, George ^a   Chang, Shih En ^a   Prijovich, Zeljko M ^a   Chen, Kai Chuan ^a   Roffler, Steve R ^a  

^a INSTITUTE OF BIOMEDICAL SCIENCES   (Taiwan)

Author keywords

glucuronidase; Aminopeptidase N; Ammonium sulphate; His tag; Mammalian cell; Purification

Indexed keywords

ACETIC ACID; AFFINITY CHROMATOGRAPHY; AMMONIA; ANTIBODIES; BIOCHEMISTRY; CELLS; ENZYMES; PRECIPITATION (CHEMICAL);

FIBROBLAST CLONES; MAMMALIAN CELLS; MAMMALIAN PROTEINS; MURINE FIBROBLASTS;

PROTEINS;

AMMONIUM SULFATE; BETA GLUCURONIDASE; HYBRID PROTEIN; MICROSOMAL AMINOPEPTIDASE; NITRILOTRIACETIC ACID; POLYHISTIDINE; PROTEIN A; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; MOUSE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION;

ANIMALS; BALB 3T3 CELLS; CELL CULTURE TECHNIQUES; CELL LINE; CLONING, MOLECULAR; HUMANS; MICE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

ANIMALIA; MAMMALIA; MURINAE;

EID: 5444224599     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20030184     Document Type: Article

References (25)

1. Seite, O. (2000) ChemBioChem 1, 214-246
2. Shipley, J. M., Grubb, J. H. and Sly, W. S. (1993) J. Biol. Chem. 268, 12193-12198
3. Liao, K. W., Lo, Y C. and Roffler, S. R. (2000) Gene Ther. 7, 339-347
4. Cheng, T. L., Wu, P. Y., Wu, M. F., Chern, J. W. and Roffler, S. R. (1999) Bioconjug. Chem. 10, 520-528
5. Cheng, T. L., Chen, B. M., Chan, L. Y., Wu, P. Y., Chern, J. W. and Roffler, S. R. (1997) Cancer Immunol. Immunother. 44, 305-315
6. Yeh, M. Y., Roffler, S. R. and Yu, M. H. (1992) Int. J. Cancer 51, 274-282
7. Pohlmann, R., Boeker, M. W. and von Figura, K. (1995) J. Biol. Chem. 270, 27311-27318
8. Haisma, H. J., Brakenhoff, R. H., van der Meulen-Muileman, I., Pinedo, H. M. and Boven, E. (1998) Cancer Immunol. Immunother. 45, 266-272
9. Haisma, H. J., Sernee, M. F., Hooijberg, E., Brakenhoff, R. H., van der Meulen-Muileman, I. H., Pinedo, H. M. and Boven, E. (1998) Blood 92, 184-190
10. Sands, M. S., Vogler, C. A., Ohlemiller, K. K., Roberts, M. S., Grubb, J. H., Levy, B. and Sly, W. S. (2001) J. Biol. Chem. 276, 43160-43165
11. Sperker, B., Murdter, T. E., Backman, J. T., Fritz, P. and Kroemer, H. K. (2002) Life Sci. 71, 1547-1557
12. Altmann, F., Staudachen E., Wilson, I. B. and Marz, L. (1999) Glycoconj. J. 16, 109-123
13. Sly, W. S., Quinton, B. A., McAlister, W. H. and Rimoin, D. L. (1973) J. Pediatr. 82, 249-257
14. Chen, B. M., Cheng, T. L., Tzou, S. C. and Roffler, S. R. (2001) Int. J. Cancer 94, 850-858
15. Ikeda, N., Nakajima, Y., Tokuhara, T., Hattori, N., Sho, M., Kanehiro, H. and Miyake, M. (2003) Clin. Cancer Res. 9, 1503-1508
16. Pasqualini, R., Koivunen, E., Kain, R., Lahdenranta, J., Sakamoto, M., Stryhn, A., Ashmun, R. A., Shapiro, L. H., Arap, W. and Ruoslahti, E. (2000) Cancer Res. 60, 722-727
17. Qiaexpressionist (2001) Handbook for High-level Expression and Purification of 6xHis-tagged Proteins, 5th edn, Qiagen, Valencia, CA
18. Cutler, P. (2003) Proteomics 3, 3-18
19. Hanash, S. (2003) Nature (London) 422, 226-232
20. Gupta, G. S. and Singh, G. P. (1983) Biochim. Biophys. Acta 748, 398-404
21. Gehrmann, M. C., Opper, M., Sedlacek, H. H., Bosslet, K. and Czech, J. (1994) Biochem. J. 301, 821-828
22. Lin, C. W., Orcutt, M. L. and Fishman, W. H. (1975) J. Biol. Chem. 250, 4737-4743
23. Lusis, A. J. and Paigen, K. (1978) J. Biol. Chem. 253, 7336-7345
24. Huang, K., Takahara, S., Kinouchi, T., Takeyama, M., Ishida, T., Ueyama, H., Nishi, K. and Ohkubo, I. (1997) J. Biochem. (Tokyo) 122, 779-787
25. Ishii, K., Usui, S., Sugimura, Y., Yoshida, S., Hioki, T., Tatematsu, M., Yamamoto, H. and Hirano, K. (2001) Int. J. Cancer 92, 49-54