Easy preparation of a large-size random gene mutagenesis library in Escherichia coli

Analytical Biochemistry

Volumn 428, Issue 1, 2012, Pages 7-12

  You, Chun ^a   Percival Zhang, Y H ^a,b,c,d  

^a State University   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c DOE   (United States)

^d Gate Fuels   (United States)

Author keywords

Cherry fluorescent protein; Directed evolution; Escherichia coli; High transformation efficiency; Prolonged overlap extension PCR; Simple cloning

Indexed keywords

CLONE CELLS; DNA; EFFICIENCY; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE; LINEAR TRANSFORMATIONS;

DIRECTED EVOLUTION; ENZYME EVOLUTIONS; FLUORESCENT PROTEIN; ORDERS OF MAGNITUDE; OVERLAP EXTENSION POLYMERASE CHAIN REACTIONS; PROLONGED OVERLAP EXTENSION PCR; RESTRICTION ENZYMES; TRANSFORMATION EFFICIENCY;

POLYMERASE CHAIN REACTION;

DNA FRAGMENT;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; DNA SHUFFLING; DNA TEMPLATE; ESCHERICHIA COLI; GENE LIBRARY; MOLECULAR WEIGHT; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROLONGED OVERLAP EXTENSION POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 84862999874     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.05.022     Document Type: Article

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