Rapid sequence scanning mutagenesis Using in Silico oligo design and the megaprimer PCR of whole plasmid method (MegaWHOP)

Methods in Molecular Biology

Volumn 634, Issue , 2010, Pages 127-135

  Krauss, Ulrich ^a   Jaeger, Karl Erich ^a   Eggert, Thorsten ^a  

^a NONE

Author keywords

Directed evolution; Saturation mutagenesis; Sequence scanning mutagenesis; Structure function studies

Indexed keywords

ESCHERICHIA COLI;

PRIMER DNA;

ARTICLE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETICS; METHODOLOGY; MUTAGENESIS; PLASMID; POLYMERASE CHAIN REACTION;

DIRECTED MOLECULAR EVOLUTION; DNA PRIMERS; ESCHERICHIA COLI; MUTAGENESIS; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 80053074108     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-652-8_9     Document Type: Article

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