Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis

Microbial Biotechnology

Volumn 4, Issue 1, 2011, Pages 98-105

  Zhang, Xiao Zhou ^a   Zhang, Y H Percival ^a,b,c  

^a State University   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c BioEnergy Science Center of Department of Energy   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CELLULASE; PROTEIN BSCEL5; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BACTERIUM TRANSFORMATION; DNA TEMPLATE; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION;

BACILLUS SUBTILIS; CELLULASE; DIRECTED MOLECULAR EVOLUTION; EVOLUTION, MOLECULAR; GENE TRANSFER TECHNIQUES; PLASMIDS; TRANSFORMATION, BACTERIAL;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 79951667638     PISSN: 17517907     EISSN: 17517915     Source Type: Journal    
DOI: 10.1111/j.1751-7915.2010.00230.x     Document Type: Article

References (37)

1. Amin, N.S., Wong, S., and Schellenberger, V. (2003) Direct transformation of site-saturation libraries in Bacillus subtilis. Biotechniques 35: 1134-1136, 1138, 1140.
2. Ara, K., Ozaki, K., Nakamura, K., Yamane, K., Sekiguchi, J., and Ogasawara, N. (2007) Bacillus minimum genome factory: effective utilization of microbial genome information. Biotechnol Appl Biochem 46: 169-178.
3. Burkholder, P.R., and Giles, N.H., Jr (1947) Induced biochemical mutations in Bacillus subtilis. Am J Bot 34: 345-348.
4. Canosi, U., Morelli, G., and Trautner, T.A. (1978) The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Mol Gen Genet 166: 259-267.
5. Caspers, M., Brockmeier, U., Degering, C., Eggert, T., and Freudl, R. (2010) Improvement of Sec-dependent secretion of a heterologous model protein in Bacillus subtilis by saturation mutagenesis of the N-domain of the AmyE signal peptide. Appl Microbiol Biotechnol 86: 1877-1885.
6. Cold Spring Harbor Protocols (2006) Using deoxycholate and trichloroacetic acid to concentrate proteins and remove interfering substances. Cold Spring Harb Protoc. doi:10.1101/pdb.prot4258.
7. Collier, K.D., Kean, A.C., Lee, E., Leiva, N., Morrison, T.B., Schellenberger, V., and Selifonova, O. (2003) Phosphate limited inducible promoter and a Bacillus expression system. World patent: WO/2003/054140.
8. Crameri, A., Raillard, S.-A., Bermudez, E., and Stemmer, W.P.C. (1998) DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature 391: 288-291.
9. Cutting, S.M., and Vander Horn, P.B. (eds) (1990) Molecular Biological Methods for Bacillus. Chichester, UK: John Wiley & Sons.
10. Dunn, A.K., and Handelsman, J. (1999) A vector for promoter trapping in Bacillus cereus. Gene 226: 297-305.
11. Ehrlich, S.D. (1978) DNA cloning in Bacillus subtilis. Proc Natl Acad Sci USA 75: 1433-1436.
12. Hartl, B., Wehrl, W., Wiegert, T., Homuth, G., and Schumann, W. (2001) Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes. J Bacteriol 183: 2696-2699.
13. Hong, J., Ye, X., Wang, Y., and Zhang, Y.-H.P. (2008) Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Anal Chim Acta 621: 193-199.
14. Maamar, H., and Dubnau, D. (2005) Bistability in the Bacillus subtilis K-state (competence) system requires a positive feedback loop. Mol Microbiol 56: 615-624.
15. Mironczuk, A.M., Kovács, Á.T., and Kuipers, O.P. (2008) Induction of natural competence in Bacillus cereus ATCC14579. Microb Biotechnol 1: 226-235.
16. Naki, D., Paech, C., Ganshaw, G., and Schellenberger, V. (1998) Selection of a subtilisin-hyperproducing Bacillus in a highly structured environment. Appl Microbiol Biotechnol 49: 290-294.
17. Phan, T.T., Nguyen, H.D., and Schumann, W. (2006) Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr Purif 46: 189-195.
18. Romero, P.A., and Arnold, F.H. (2009) Exploring protein fitness landscapes by directed evolution. Nat Rev Mol Cell Biol 10: 866-876.
19. Sambrook, J., and Russel, D.W. (2001) Molecular Cloning: A Laboratory Manual. New York, USA: Cold Spring Harbor Laboratory.
20. Schumann, W. (2007) Production of recombinant proteins in Bacillus subtilis. Adv Appl Microbiol 62: 137-189.
21. Shafikhani, S., Siegel, R.A., Ferrari, E., and Schellenberger, V. (1997) Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization. Biotechniques 23: 304-310.
22. Smits, W.K., Eschevins, C.C., Susanna, K.A., Bron, S., Kuipers, O.P., and Hamoen, L.W. (2005) Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development. Mol Microbiol 56: 604-614.
23. Strausberg, S.L., Alexander, P.A., Gallagher, D.T., Gilliland, G.L., Barnett, B.L., and Bryan, P.N. (1995) Directed evolution of a subtilisin with calcium-independent stability. Biotechnology (N Y) 13: 669-673.
24. Susanna, K.A., Fusetti, F., Thunnissen, A.-M.W.H., Hamoen, L.W., and Kuipers, O.P. (2006) Functional analysis of the competence transcription factor ComK of Bacillus subtilis by characterization of truncation variants. Microbiology 152: 473-483.
25. Tjalsma, H., Bolhuis, A., Jongbloed, J.D., Bron, S., and van Dijl, J.M. (2000) Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome. Microbiol Mol Biol Rev 64: 515-547.
26. Tjalsma, H., Antelmann, H., Jongbloed, J.D., Braun, P.G., Darmon, E., Dorenbos, R., etal. (2004) Proteomics of protein secretion by Bacillus subtilis: separating the 'secrets' of the secretome. Microbiol Mol Biol Rev 68: 207-233.
27. Wolf, M., Geczi, A., Simon, O., and Borriss, R. (1995) Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase. Microbiology 141 (Part 2): 281-290.
28. Xue, G.-P., Johnson, J.S., and Dalrymple, B.P. (1999) High osmolarity improves the electro-transformation efficiency of the gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J Microbiol Methods 34: 183-191.
29. Yamane, K., Bunai, K., and Kakeshita, H. (2004) Protein traffic for secretion and related machinery of Bacillus subtilis. Biosci Biotechnol Biochem 68: 2007-2023.
30. You, L., and Arnold, F.H. (1996) Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng 9: 77-83.
31. Zhang, X.-Z., and Zhang, Y.-H.P. (2010) One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: opportunities and challenges. Eng Life Sci 10: 398-406.
32. Zhang, X.-Z., Cui, Z.-L., Hong, Q., and Li, S.-P. (2005) High-level expression and secretion of methyl parathion hydrolase in Bacillus subtilis WB800. Appl Environ Microbiol 71: 4101-4103.
33. Zhang, X.-Z., Zhang, Z., Zhu, Z., Sathitsuksanoh, N., Yang, Y., and Zhang, Y.H.P. (2010) The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity. Appl Microbiol Biotechnol 86: 525-533.
34. Zhang, Y.-H.P., and Lynd, L.R. (2005) Determination of the number-average degree of polymerization of cellodextrins and cellulose with application to enzymatic hydrolysis. Biomacromolecules 6: 1510-1515.
35. Zhang, Y.-H.P., Himmel, M., and Mielenz, J.R. (2006a) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24: 452-481.
36. Zhang, Y.-H.P., Cui, J.-B., Lynd, L.R., and Kuang, L.R. (2006b) A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidences from enzymatic hydrolysis and supramolecular structure. Biomacromolecules 7: 644-648.
37. Zhao, H., and Arnold, F.H. (1999) Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Eng 12: 47-53.