Knockdown of HURP inhibits the proliferation of hepacellular carcinoma cells via downregulation of gankyrin and accumulation of p53

Biochemical Pharmacology

Volumn 83, Issue 6, 2012, Pages 758-768

  Kuo, Tzu Ching ^a   Chang, Pu Yuan ^a   Huang, Shiu Feng ^b   Chou, Chen Kung ^a   Chao, Chuck C K ^a  

^a CHANG GUNG UNIVERSITY   (Taiwan)

^b NATIONAL HEALTH RESEARCH INSTITUTES   (Taiwan)

Author keywords

Chemoresistance; Gankyrin; Hepatocellular carcinoma; HURP; Oncogenesis; p53

Indexed keywords

CISPLATIN; GANKYRIN; HEPATOMA UPREGULATED PROTEIN; MESSENGER RNA; PROTEIN; PROTEIN MDM2; PROTEIN P53; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CARCINOMA CELL; CELL PROLIFERATION; CELL STRAIN HEK293; CELL TRANSFORMATION; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; HUMAN; HUMAN CELL; IN VIVO STUDY; LIVER CELL CARCINOMA; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; TUMOR GROWTH; UBIQUITINATION; UPREGULATION; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; CARCINOMA, HEPATOCELLULAR; CELL ADHESION; CELL LINE, TUMOR; CELL PROLIFERATION; CISPLATIN; DOWN-REGULATION; DRUG RESISTANCE, NEOPLASM; FEMALE; HEK293 CELLS; HUMANS; LIVER NEOPLASMS; MICE; MICE, NUDE; NEOPLASM PROTEINS; PROTEASOME ENDOPEPTIDASE COMPLEX; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MDM2; RNA, SMALL INTERFERING; TUMOR SUPPRESSOR PROTEIN P53; UBIQUITINATION; UP-REGULATION;

EID: 84862832334     PISSN: 00062952     EISSN: 18732968     Source Type: Journal    
DOI: 10.1016/j.bcp.2011.12.034     Document Type: Article

References (36)

1. A.P. Tsou, C.W. Yang, C.Y. Huang, R.C. Yu, Y.C. Lee, and C.W. Chang Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma Oncogene 22 2003 298 307 (Pubitemid 36174187)
2. M.D. Koffa, C.M. Casanova, R. Santarella, T. Kocher, M. Wilm, and I.W. Mattaj HURP is part of a Ran-dependent complex involved in spindle formation Curr Biol 16 2006 743 754
3. H.H. Sillje, S. Nagel, R. Korner, and E.A. Nigg HURP is a Ran-importin beta-regulated protein that stabilizes kinetochore microtubules in the vicinity of chromosomes Curr Biol 16 2006 731 742
4. J. Wong, and G. Fang HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture J Cell Biol 173 2006 879 891
5. C.T. Yu, J.M. Hsu, Y.C. Lee, A.P. Tsou, C.K. Chou, and C.Y. Huang Phosphorylation and stabilization of HURP by Aurora-A: implication of HURP as a transforming target of Aurora-A Mol Cell Biol 25 2005 5789 5800
6. J. Wong, R. Lerrigo, C.Y. Jang, and G. Fang Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain Mol Biol Cell 19 2008 2083 2091
7. J.M. Hsu, Y.C. Lee, C.T. Yu, and C.Y. Huang Fbx7 functions in the SCF complex regulating Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis by a proline-rich region J Biol Chem 279 2004 32592 32602
8. D. Hanahan, and R.A. Weinberg The hallmarks of cancer Cell 100 2000 57 70 (Pubitemid 30046295)
9. B. Vogelstein, D. Lane, and A.J. Levine Surfing the p53 network Nature 408 2000 307 310
10. Y. Yang, C.C. Li, and A.M. Weissman Regulating the p53 system through ubiquitination Oncogene 23 2004 2096 2106 (Pubitemid 38496739)
11. D. Michael, and M. Oren The p53-Mdm2 module and the ubiquitin system Semin Cancer Biol 13 2003 49 58 (Pubitemid 36269180)
12. H. Higashitsuji, K. Itoh, T. Nagao, S. Dawson, K. Nonoguchi, and T. Kido Reduced stability of retinoblastoma protein by gankyrin, an oncogenic ankyrin-repeat protein overexpressed in hepatomas Nat Med 6 2000 96 99 (Pubitemid 30048534)
13. S. Dawson, S. Apcher, M. Mee, H. Higashitsuji, R. Baker, and S. Uhle Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome J Biol Chem 277 2002 10893 10902 (Pubitemid 34952844)
14. H. Higashitsuji, K. Itoh, T. Sakurai, T. Nagao, Y. Sumitomo, and T. Masuda The oncoprotein gankyrin binds to MDM2/HDM2, enhancing ubiquitylation and degradation of p53 Cancer Cell 8 2005 75 87 (Pubitemid 40991816)
15. W. Qiu, J. Wu, E.M. Walsh, Y. Zhang, C.Y. Chen, and J. Fujita Retinoblastoma protein modulates gankyrin-MDM2 in regulation of p53 stability and chemosensitivity in cancer cells Oncogene 27 2008 4034 4043 (Pubitemid 351931989)
16. Y.H. Ou, P.H. Chung, T.P. Sun, and S.Y. Shieh p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation Mol Biol Cell 16 2005 1684 1695
17. N.K. Sun, H.P. Lu, and C.C. Chao Identification of rat DDB1, a putative DNA repair protein, and functional correlation with its damaged-DNA recognition activity J Biomed Sci 9 2002 371 380 (Pubitemid 41582725)
18. C.L. Sun, and C.C. Chao Cross-resistance to death ligand-induced apoptosis in cisplatin-selected HeLa cells associated with overexpression of DDB2 and subsequent induction of cFLIP Mol Pharmacol 67 2005 1307 1314 (Pubitemid 40410431)
19. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
20. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
21. C.C. Chao, Y.L. Lee, P.W. Cheng, and S. Lin-Chao Enhanced host cell reactivation of damaged plasmid DNA in HeLa cells resistant to cis-diamminedichloroplatinum(II) Cancer Res 51 1991 601 605
22. Z.Z. Wu, and C.C. Chao Knockdown of NAPA using short-hairpin RNA sensitizes cancer cells to cisplatin: implications to overcome chemoresistance Biochem Pharmacol 80 2010 827 837
23. T.C. Kuo, and C.C. Chao Hepatitis B virus X protein prevents apoptosis of hepatocellular carcinoma cells by upregulating SATB1 and HURP expression Biochem Pharmacol 80 2010 1093 1102
24. T. Riley, E. Sontag, P. Chen, and A. Levine Transcriptional control of human p53-regulated genes Nat Rev Mol Cell Biol 9 2008 402 412 (Pubitemid 351574202)
25. C.L. Wei, Q. Wu, V.B. Vega, K.P. Chiu, P. Ng, and T. Zhang A global map of p53 transcription-factor binding sites in the human genome Cell 124 2006 207 219 (Pubitemid 43069320)
26. A. Puisieux, K. Galvin, F. Troalen, B. Bressac, C. Marcais, and E. Galun Retinoblastoma and p53 tumor suppressor genes in human hepatoma cell lines FASEB J 7 1993 1407 1413 (Pubitemid 23336171)
27. H.V. Clegg, K. Itahana, and Y. Zhang Unlocking the Mdm2-p53 loop Cell Cycle 7 2008 1 6
28. Y. Xu Regulation of p53 responses by post-translational modifications Cell Death Differ 10 2003 400 403 (Pubitemid 36626351)
29. L. Feng, T. Lin, H. Uranishi, W. Gu, and Y. Xu Functional analysis of the roles of posttranslational modifications at the p53C terminus in regulating p53 stability and activity Mol Cell Biol 25 2005 5389 5395 (Pubitemid 40853576)
30. K.A. Krummel, C.J. Lee, F. Toledo, and G.M. Wahl The C-terminal lysines fine-tune P53 stress responses in a mouse model but are not required for stability control or transactivation Proc Natl Acad Sci USA 102 2005 10188 10193 (Pubitemid 41023347)
31. C.L. Brooks, and W. Gu p53 ubiquitination: Mdm2 and beyond Mol Cell 21 2006 307 315 (Pubitemid 43163526)
32. S. Yamasaki, N. Yagishita, T. Sasaki, M. Nakazawa, Y. Kato, and T. Yamadera Cytoplasmic destruction of p53 by the endoplasmic reticulum-resident ubiquitin ligase 'synoviolin' EMBO J 26 2007 113 122 (Pubitemid 46094704)
33. Z.Z. Wu, N.K. Sun, K.Y. Chien, and C.C. Chao Silencing of the SNARE protein NAPA sensitizes cancer cells to cisplatin by inducing ERK1/2 signaling, synoviolin ubiquitination and p53 accumulation Biochem Pharmacol 82 2011 1630 1640
34. Y. Aylon, and M. Oren Living with p53, dying of p53 Cell 130 2007 597 600 (Pubitemid 47268063)
35. T. Tanaka, S. Ohkubo, I. Tatsuno, and C. Prives hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes Cell 130 2007 638 650 (Pubitemid 47268061)
36. S. Das, L. Raj, B. Zhao, Y. Kimura, A. Bernstein, and S.A. Aaronson Hzf Determines cell survival upon genotoxic stress by modulating p53 transactivation Cell 130 2007 624 637 (Pubitemid 47277418)