A 24-microwell plate with improved mixing and scalable performance for high throughput cell cultures

Process Biochemistry

Volumn 47, Issue 4, 2012, Pages 612-618

  Wen, Yuan ^a   Zang, Ru ^a   Zhang, Xudong ^a   Yang, Shang Tian ^a  

^a The Ohio State University   (United States)

Author keywords

24 Microwell plate; Cell culture; High throughput; Mixing, aeration, CHO

Indexed keywords

24-MICROWELL PLATE; AGITATION RATES; ANTIBODY PRODUCTION; BIOTECHNOLOGY INDUSTRY; CELL AGGREGATION; CELL CLONES; CELL CULTURE OPTIMIZATION; CELL CULTURE PROCESS; CHO CELL; DATA QUALITY; DRUG SCREENING; GLUCOSE CONSUMPTION; HIGH THROUGHPUT; HIGH THROUGHPUT SCREENING; OXYGEN TRANSFER; POTENTIAL APPLICATIONS; REPRODUCIBILITIES; SCALABLE PERFORMANCE; SERUM-FREE MEDIUM; SPECIFIC GROWTH RATE; SPINNER FLASKS; STANDARD DEVIATION; STATIC MIXERS;

BIOACTIVITY; BOTTLES; CELL CULTURE; CELL PROLIFERATION; EVAPORATION; GROWTH KINETICS; MICROCHANNELS; MIXERS (MACHINERY); PHASE TRANSITIONS; THROUGHPUT;

MIXING;

EID: 84862802734     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2011.12.023     Document Type: Article

References (38)

1. B. Leader, Q.J. Baca, and D.E. Golan Protein therapeutics: a summary and pharmacological classification Nat Rev Drug Discov 7 2008 21 39
2. A. Nelson, E. Dhimolea, and J.M. Reichert Development trends for human monoclonal antibody therapeutics Nat Rev Drug Discov 9 2010 767 774
3. M. Micheletti, and G.J. Lye Microscale bioprocess optimisation Curr Opin Biotechnol 17 2006 611 618 (Pubitemid 44791911)
4. R. Bhambure, K. Kumar, and A.S. Rathore High-throughput process development for biopharmaceutical drug substance Trends Biotechnol 29 2011 127 135
5. F. Kensy, G.T. John, B. Hofmann, and J. Buchs Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates Bioprocess Biosyst Eng 28 2005 75 81 (Pubitemid 41749729)
6. Y. Kostov, P. Harms, L. Randers-Eichhorn, and G. Rao Low-cost microbioreactor for high-throughput bioprocessing Biotechnol Bioeng 72 2001 346 352 (Pubitemid 32099587)
7. H.L. Lee, P. Boccazzi, R.J. Ram, and A.J. Sinskey Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control Lab Chip 6 2006 1229 1235 (Pubitemid 44290155)
8. M.M. Maharbiz, W.J. Holtz, R.T. Howe, and J.D. Keasling Microbioreactor arrays with parametric control for high-throughput experimentation Biotechnol Bioeng 86 2004 485 490
9. N. Szita, P. Boccazzi, Z. Zhang, P. Boyle, A.J. Sinskey, and K.F. Jensen Development of a multiplexed microbioreactor system for high-throughput bioprocessing Lab Chip 5 2005 819 826 (Pubitemid 41117308)
10. A. Zanzotto, N. Szita, P. Boccazzi, P. Lessard, A.J. Sinskey, and K.F. Jensen Membrane-aerated microbioreactor for high-throughput bioprocessing Biotechnol Bioeng 87 2004 243 254 (Pubitemid 38879755)
11. Z. Zhang, P. Boccazzi, H.G. Choi, G. Perozziello, A.J. Sinskey, and K.F. Jensen Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor Lab Chip 6 2006 906 913 (Pubitemid 43962798)
12. Z. Zhang, N. Szita, P. Boccazzi, A.J. Sinskey, and K.F. Jensen A well-mixed, polymer-based microbioreactors with integrated optical measurements Biotechnol Bioeng 93 2006 286 296
13. R. Legmann, H.B. Schreyer, R.G. Combs, E.L. McCormick, A.P. Russo, and S.T. Rodgers A predictive High-Throughput Scale-Down model of monoclonal antibody production in CHO cells Biotechnol Bioeng 104 2009 1107 1120
14. L.M. Chiverton Miniaturization-Micro scale bioprocess development Immunotherapy 3 2011 13 16
15. R. Bareither, and D. Pollard A review of advanced small-scale parallel bioreactor technology for accelerated process development: current state and future need Biotechnol Prog 27 2011 2 14
16. A. Chen, R. Chitta, D. Chang, and A. Amanullah Twenty-four well plate miniature bioreactor system as a scale-down model for cell culture process development Biotechnol Bioeng 102 2009 148 160
17. K. Isett, H. George, W. Herber, and A. Amanullah Twenty-four-well plate miniature bioreactor high-throughput system: assessment for microbial cultivations Biotechnol Bioeng 98 2007 1017 1028 (Pubitemid 350205579)
18. R.R. Deshpande, C. Wittmann, and E. Heinzle Microplates with integrated oxygen sensing for medium optimization in animal cell culture Cytotechnology 46 2004 1 8 (Pubitemid 40897990)
19. W.A. Duetz Microtiter plates as mini-bioreactors: miniaturization of fermentation methods Trends Microbiol 15 2007 469 475 (Pubitemid 350026353)
20. A.S. Kocincova, S. Nagl, S. Arain, C. Krause, S.M. Borisov, and M. Arnold Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH Biotechnol Bioeng 100 2008 430 438 (Pubitemid 351792919)
21. A. Amanullah, J.M. Otero, M. Mikola, J.A. Hope, H.B. Schreyer, and J. Aunins Novel microbioreactor high throughput technology for cell culture process development: reproducibility and scalability assessment fed-batch CHO cultures Biotechnol Bioeng 106 2010 57 67
22. S. Weiss, G.T. John, I. Klimant, and E. Heinzle Modeling of mixing in 96-well microplates observed with fluorescence indicators Biotechnol Prog 18 2002 821 830 (Pubitemid 34853394)
23. P. Girard, M. Jordan, M. Tsao, and F.M. Wurm Small-scale bioreactor system for process development and optimization Biochem Eng J 7 2001 117 119 (Pubitemid 32162698)
24. C. Heath, and R. Kiss Cell culture process development: advances in process engineering Biotechnol Prog 23 2007 46 51 (Pubitemid 46293485)
25. B. Kondragunta, J.L. Drew, K.A. Brorson, A.R. Moreira, and G. Rao Advances in clone selection using high-throughput bioreactors Biotechnol Prog 26 2010 1095 1103
26. J. Diao, L. Young, P. Zhou, and M.L. Shuler An actively mixed mini-bioreactor for protein production from suspended animal cells Biotechnol Bioeng 100 2008 72 81 (Pubitemid 351579017)
27. E.L. Paul, V.A. Atiemo-Obeng, and S.M. Kresta Handbook of industrial mixing: science and practice 2004 John Wiley & Sons Hoboken
28. R. Hermann, N. Walther, U. Maier, and J. Buchs Optical method for the determination of the oxygen-transfer capacity of small bioreactors based on sulfite oxidation Biotechnol Bioeng 74 2001 355 363 (Pubitemid 32777451)
29. R. Hermann, M. Lehmann, and J. Buchs Characterization of gas-liquid mass transfer phenomena in microtiter plates Biotechnol Bioeng 81 2003 178 186 (Pubitemid 36077294)
30. S.D. Doig, S.C.R. Pickering, G.J. Lye, and F. Baganz Modelling surface aeration rates in shaken microtitre plates using dimensionless groups Chem Eng Sci 60 2005 2741 2750 (Pubitemid 40393747)
31. T.A. Barrett, A. Wu, H. Zhang, M.S. Levy, and G.J. Lye Microwell engineering characterization for mammalian cell culture process development Biotechnol Bioeng 105 2010 260 275
32. R.A. Taticek Enhanced recombinant protein expression in baculovirus-infected high-density insect cell suspension cultures and the operation of a continuous flow bioreactor 1995 Cornell University Ithaca
33. M.L. Shuler, and F. Kargi Bioprocess engineering: basic concepts 2nd ed. 2002 Prentice Hall Upper Saddle River, NJ
34. S. Ostrovidov, J. Jiang, Y. Sakai, and T. Fujii Membrane-based PDMS microbioreactor for perfused 3D primary rat hepatocyte cultures Biomed Microdevices 6 2004 279 287 (Pubitemid 39545747)
35. X. Zhang, and S.T. Yang High-throughput 3-D cell-based proliferation and cytotoxicity assays for drug screening and bioprocess development J Biotechnol 151 2011 186 193
36. M. Micheletti, T. Barret, S.D. Doig, F. Baganz, M.S. Levy, and J.M. Woodley Fluid mixing in shaken bioreactors: implications for scale-up predictions from microliter-scale microbial and mammalian cell cultures Chem Eng Sci 61 2006 2939 2949 (Pubitemid 43277397)
37. C.A. Reznikoff, D.W. Brankow, and C. Heidelberger Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division Cancer Res 33 1973 3231 3238
38. R.E. Berson, M.R. Purcell, and M.K. Sharp Computationally determined shear on cells grown in orbiting culture dishes Adv Exp Med Biol 614 2008 189 198