A combination of flow cytometry and traditional screening using chemicals to isolate high glutathione-producing yeast mutants

Bioscience, Biotechnology and Biochemistry

Volumn 76, Issue 6, 2012, Pages 1085-1090

  Nishiuchi, Hiroaki ^a   Tabira, Yukiko ^a   Yamagishi, Kazuo ^a  

^a AJINOMOTO CO INC   (Japan)

Author keywords

5 Chloromethyl fluorescein diacetate (CMFDA); Efficient screening method; Glutathione (GSH) producing yeast

Indexed keywords

5-CHLOROMETHYL-FLUORESCEIN DIACETATE (CMFDA); CERULENIN; FLUORESCENCE INTENSITIES; FOOD PRODUCTION; GLUTATHIONES; PARENTAL STRAINS; SACCHAROMYCES CEREVISIAE STRAINS; SCREENING EFFICIENCY; SCREENING METHODS; YEAST STRAIN;

CHEMICAL CONTAMINATION; CHEMICALS; FLOW CYTOMETRY; PEPTIDES;

YEAST;

5 CHLOROMETHYLFLUORESCEIN; 5-CHLOROMETHYLFLUORESCEIN; CERULENIN; FATTY ACID SYNTHASE INHIBITOR; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; GLUTATHIONE;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; FLOW CYTOMETRY; FOOD HANDLING; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; SACCHAROMYCES CEREVISIAE;

CERULENIN; FATTY ACID SYNTHESIS INHIBITORS; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; FOOD TECHNOLOGY; GLUTATHIONE; HIGH-THROUGHPUT SCREENING ASSAYS; MUTATION; SACCHAROMYCES CEREVISIAE;

EID: 84862749525     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.110883     Document Type: Article

References (22)

1. Ueda Y, Sakaguchi M, Hirayama K, Miyajima R, and Kimizuka A, Agric. Biol. Chem., 54, 163-169 (1990).
2. Kuroda M, Nagaba H, Tsubuku T, Kawajiri H, and Ueda Y, Food Sci. Technol. Int. Tokyo, 3, 239-240 (1997).
3. Li Y, Wei G, and Chen J, Appl. Microbiol. Biotechnol., 66, 233-242 (2004).
4. Ueda Y, Yonemitsu M, Tsubuku T, Sakaguchi M, and Miyajima R, Biosci. Biotechnol. Biochem., 61, 1977-1980 (1997).
5. Ohtake Y and Yabuuchi S, Yeast, 7, 953-961 (1991).
6. Inoue Y, Sugiyama K, Izawa S, and Kimura A, Biochim. Biophys. Acta, 1395, 315-320 (1998).
7. Sofyanovich OA, Nishiuchi H, Yamagishi K, Maekawa K, and Serebryanyy VA, Mol. Biotechnol., 48, 218-227 (2011).
8. Wang Z, Zhang L, and Tan T, Process Biochem., 45, 1168-1171 (2010).
9. Sherman F, Methods Enzymol., 350, 3-41 (2002).
10. Erdeniz N, Mortensen UH, and Rothstein R, Genome Res., 7, 1174-1183 (1997).
11. Wach A, Yeast, 12, 259-265 (1996).
12. Shevchuk NA, Bryksin AV, Nushinovich YA, Cabello FC, Sutherland M, and Ladisch S, Nucleic Acids Res., 32, e19 (2004).
13. Nishiuchi H, Umezawa Y, and Wakabayashi H, Food Sci. Technol. Res., 17, 573-577 (2011).
14. Thomas D, Kuras L, Barbey R, Cherest H, Blaiseau PL, and Surdin-Kerjan Y, Mol. Cell. Biol., 15, 6526-6534 (1995).
15. Rouillon A, Barbey R, Patton EE, Tyers M, and Thomas D, EMBO J., 19, 282-294 (2000).
16. Wheeler GL, Quinn KA, Perrone G, Dawes IW, and Grant CM, Mol. Microbiol., 2, 545-556 (2002).
17. Yen JL, Su NY, and Kaiser P, Mol. Biol. Cell, 16, 1872-1882 (2005).
18. Cherest H and Surdin-Kerjan Y, Genetics, 130, 51-58 (1992).
19. Oskouian B and Saba JD, Mol. Gen. Genet., 261, 346-353 (1999).
20. Wu AL and Moye-Rowley WS, Mol. Cell. Biol., 14, 5832-5839 (1994).
21. Inoue Y, Choi B-Y, Murata K, and Kimura A, Biochem. Biophys. Res. Commun., 165, 1091-1095 (1989).
22. Ikushima S, Fujii T, and Kobayashi O, Biosci. Biotechnol. Biochem., 73, 879-884 (2009).