A new method for repeated "self-Cloning" promoter replacement in Saccharomyces cerevisiae

Molecular Biotechnology

Volumn 48, Issue 3, 2011, Pages 218-227

  Sofyanovich, Olga A ^a   Nishiuchi, Hiroaki ^b   Yamagishi, Kazuo ^b   Maekawa, Kenjiro ^b   Serebryanyy, Vsevolod A ^a  

^a Ajinomoto Genetika Research Institute   (Russian Federation)

^b AJINOMOTO CO INC   (Japan)

Author keywords

PCR; Promoter replacement; Saccharomyces cerevisiae; Self cloning

Indexed keywords

DNA FRAGMENT; HOMOLOGOUS RECOMBINATION; INDUSTRIAL STRAIN; MARKER GENES; NEGATIVE SELECTION; PCR; PROMOTER REPLACEMENT; PROMOTER UPSTREAM; S.CEREVISIAE; SELF-CLONING; TARGET GENES; YEAST SACCHAROMYCES CEREVISIAE;

CLONING; GENES; INDUSTRIAL RESEARCH; POLYMERASE CHAIN REACTION;

YEAST;

DNA FRAGMENT; SACCHAROMYCES CEREVISIAE PROTEIN; URA3 PROTEIN, S CEREVISIAE;

ARTICLE; CONTROLLED STUDY; EXCISION REPAIR; FUNGAL GENE; GENE CASSETTE; GENE LOCUS; HOMOLOGOUS RECOMBINATION; MARKER GENE; MOLECULAR CLONING; NEGATIVE FEEDBACK; NONHUMAN; POLYMERASE CHAIN REACTION; POSITIVE FEEDBACK; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; TERMINAL SEQUENCE; BIOLOGICAL MODEL; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID;

SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, FUNGAL; GENETIC MARKERS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 80054034892     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-010-9362-6     Document Type: Article

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