Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 68, Issue 5, 2012, Pages 596-600

  Geders, Todd W ^a   Gustafson, Kathryn ^a   Finzel, Barry C ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Crystallization optimization; Differential scanning fluorimetry; Pyridoxal 5' phosphate; ThermoFluor; UV Vis spectroscopy

Indexed keywords

AMINOTRANSFERASE; BACTERIAL PROTEIN; BIOA PROTEIN, BACTERIA; PYRIDOXAL 5 PHOSPHATE;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; FLUOROMETRY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MYCOBACTERIUM TUBERCULOSIS;

BACTERIAL PROTEINS; CRYSTALLIZATION; FLUOROMETRY; MYCOBACTERIUM TUBERCULOSIS; PYRIDOXAL PHOSPHATE; TRANSAMINASES;

EID: 84862516771     PISSN: None     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309112012912     Document Type: Article

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