A stepwise approach for the reproducible optimization of PAMO expression in Escherichia coli for whole-cell biocatalysis

BMC Biotechnology

Volumn 12, Issue , 2012, Pages

  van Bloois, Edwin ^a,b   Dudek, Hanna M ^a   Duetz, Wouter A ^b   Fraaije, Marco W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b Enzyscreen BV   (Netherlands)

Author keywords

Baeyer villiger monoxygenase; Biocatalysis; Escherichia coli; Screening; Square deep well microtiter plates

Indexed keywords

96-WELL MICROTITER PLATE; ACTIVITY-BASED; BAEYER-VILLIGER MONOOXYGENASES; BAEYER-VILLIGER MONOXYGENASE; BIOCATALYSIS; BIOCATALYTIC PERFORMANCE; ELECTRON DONORS; EXPRESSION IN ESCHERICHIA COLI; HOST STRAIN; INDUCTION PERIODS; MICROTITER PLATES; MONOOXYGENASES; OPTIMIZED PARAMETER; OPTIMIZED SYSTEM; RECOMBINANT E. COLI; RELATIVE STANDARD DEVIATIONS; SCREENING PROCEDURES; STEPWISE APPROACH; SUBSTRATE CONCENTRATIONS; SYNTHETIC APPLICATION; THERMOBIFIDA FUSCA; WHOLE-CELL BIOCATALYSIS;

BIOCATALYSTS; BIODEGRADATION; ENZYMES; ESCHERICHIA COLI; SCREENING;

OPTIMIZATION;

ACETONE; BENZENE DERIVATIVE; PHENYLACETONE; PHENYLACETONE MONOOXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; 1-PHENYL-2-PROPANONE; DRUG DERIVATIVE; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL STRAIN; BIOCATALYSIS; BIOCATALYST; BIOTRANSFORMATION; CONTROLLED STUDY; ENZYME MECHANISM; ESCHERICHIA COLI; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; REPRODUCIBILITY; THERMOBIFIDA FUSCA; THERMOPHILIC BACTERIUM; WHOLE CELL; ACTINOMYCETALES; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; TEMPERATURE; TIME;

ESCHERICHIA COLI; THERMOBIFIDA FUSCA;

ACETONE; ACTINOMYCETALES; BIOCATALYSIS; ESCHERICHIA COLI; KINETICS; MIXED FUNCTION OXYGENASES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS;

EID: 84862505690     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-12-31     Document Type: Article

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