Inhibition of cell proliferation and induction of apoptosis by oleanane triterpenoid (CDDO-Me) in pancreatic cancer cells is associated with the suppression of hTERT gene expression and its telomerase activity

Biochemical and Biophysical Research Communications

Volumn 422, Issue 4, 2012, Pages 561-567

  Deeb, Dorrah ^a   Gao, Xiaohua ^a   Liu, Yongbo ^a   Kim, Sahn Ho ^b   Pindolia, Kirit R ^a   Arbab, Ali S ^a   Gautam, Subhash C ^a  

^a HENRY FORD HEALTH SYSTEM   (United States)

^b HENRY FORD HOSPITAL   (United States)

Author keywords

Akt (protein kinase b); Apoptosis; CDDO Me; HTERT; MTS; NF B; Pancreatic cancer; PARP 1; STAT 3; Telomerase

Indexed keywords

BARDOXOLONE METHYL; TELOMERASE; TELOMERASE REVERSE TRANSCRIPTASE;

ANTIINFLAMMATORY ACTIVITY; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL PROLIFERATION; CONTROLLED STUDY; DISEASE ASSOCIATION; ENZYME ACTIVITY; GENE EXPRESSION; GENE REPRESSION; GENETIC SUSCEPTIBILITY; HUMAN; HUMAN CELL; HUMAN TISSUE; PANCREAS CANCER; PRIORITY JOURNAL; TUMOR CELL;

ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; CELL PROLIFERATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; OLEANOLIC ACID; PANCREATIC NEOPLASMS; SUPPRESSION, GENETIC; TELOMERASE;

EID: 84862284026     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.05.024     Document Type: Article

References (38)

1. Greider C.W. Chromosome first aid. Cell 1991, 67:645-647.
2. Kim N.W., Piatyszek M.A., Prowse K.R., Harley C.B., West M.D., Ho P.L., Coviello G.M., Wright W.E., Weinrich S.L., Shay J.W. Specific association of human telomerase activity with immortal cells and cancer. Science 1994, 266:2011-2015.
3. Shay J.W., Bacchetti S. A survey of telomerase activity in human cancer. Eur. J. Cancer 1997, 33:787-791.
4. Kilian A., Bowtell D.D., Abud H.E., Hime G.R., Venter D.J., Keese P.K., Duncan E.L., Reddel R.R., Jefferson R.A. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum. Mol. Genet. 1997, 6:2011-2019.
5. Feng J., Funk W.D., Wang S.S., Weinrich S.L., Avilion A.A., Chiu C.P., Adams R.R., Chang E., Allsopp R.C., Yu J., et al. The RNA component of human telomerase. Science 1995, 269:1236-1241.
6. De Boeck G., Forsyth R.G., Praet M., Hogendoorn P.C. Telomere-associated proteins: cross-talk between telomere maintenance and telomere-lengthening mechanisms. J. Pathol. 2009, 217:327-344.
7. Wright W.E., Piatyszek M.A., Rainey W.E., Byrd W., Shay J.W. Telomerase activity in human germline and embryonic tissues and cells. Dev. Genet. 1996, 18:173-179.
8. Holt S.E., Wright W.E., Shay J.W. Regulation of telomerase activity in immortal cell lines. Mol. Cell. Biol. 1996, 16:2932-2939.
9. Blasco M.A., Hahn W.C. Evolving views of telomerase and cancer. Trends Cell Biol. 2003, 13:289-294.
10. Newbold R.F. The significance of telomerase activation and cellular immortalization in human cancer. Mutagenesis 2002, 17:539-550.
11. Cech T.R. Beginning to understand the end of the chromosome. Cell 2004, 116:273-279.
12. Janknecht R. On the road to immortality: hTERT upregulation in cancer cells. FEBS Lett. 2004, 564:9-13.
13. Meyerson M., Counter C.M., Eaton E.N., Ellisen L.W., Steiner P., Caddle S.D., Ziaugra L., Beijersbergen R.L., Davidoff M.J., Liu Q., Bacchetti S., Haber D.A., Weinberg R.A. HEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 1997, 90:785-795.
14. Liby K.T., Yore M.M., Sporn M.B. Triterpenoids and rexinoids as multifunctional agents for the prevention and treatment of cancer. Nat. Rev. Cancer 2007, 7:357-369.
15. Ito Y., Pandey P., Sporn M.B., Datta R., Kharbanda S., Kufe D. The novel triterpenoid CDDO induces apoptosis and differentiation of human osteosarcoma cells by a caspase-8 dependent mechanism. Mol. Pharmacol. 2001, 59:1094-1099.
16. Konopleva M., Tsao T., Estrov Z., Lee R.M., Wang R.Y., Jackson C.E., McQueen T., Monaco G., Munsell M., Belmont J., Kantarjian H., Sporn M.B., Andreeff M. The synthetic triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid induces caspase-dependent and -independent apoptosis in acute myelogenous leukemia. Cancer Res. 2004, 64:7927-7935.
17. Gao X., Deeb D., Jiang H., Liu Y., Dulchavsky S.A., Gautam S.C. Synthetic triterpenoids inhibit growth and induce apoptosis in human glioblastoma and neuroblastoma cells through inhibition of prosurvival Akt, NF-kappaB and Notch1 signaling. J. Neurooncol. 2007, 84:147-157.
18. Konopleva M., Contractor R., Kurinna S.M., Chen W., Andreeff M., Ruvolo P.P. The novel triterpenoid CDDO-Me suppresses MAPK pathways and promotes p38 activation in acute myeloid leukemia cells. Leukemia 2005, 19:1350-1354.
19. Ahmad R., Raina D., Meyer C., Kharbanda S., Kufe D. Triterpenoid CDDO-Me blocks the NF-kappaB pathway by direct inhibition of IKKbeta on Cys-179. J. Biol. Chem. 2006, 281:35764-35769.
20. Suh N., Roberts A.B., Birkey Reffey S., Miyazono K., Itoh S., ten Dijke P., Heiss E.H., Place A.E., Risingsong R., Williams C.R., Honda T., Gribble G.W., Sporn M.B. Synthetic triterpenoids enhance transforming growth factor beta/Smad signaling. Cancer Res. 2003, 63:1371-1376.
21. Ling X., Konopleva M., Zeng Z., Ruvolo V., Stephens L.C., Schober W., McQueen T., Dietrich M., Madden T.L., Andreeff M. The novel triterpenoid C-28 methyl ester of 2-cyano-3, 12-dioxoolen-1, 9-dien-28-oic acid inhibits metastatic murine breast tumor growth through inactivation of STAT3 signaling. Cancer Res. 2007, 67:4210-4218.
22. Liby K., Royce D.B., Williams C.R., Risingsong R., Yore M.M., Honda T., Gribble G.W., Dmitrovsky E., Sporn T.A., Sporn M.B. The synthetic triterpenoids CDDO-methyl ester and CDDO-ethyl amide prevent lung cancer induced by vinyl carbamate in A/J mice. Cancer Res. 2007, 67:2414-2419.
23. Liby K.T., Royce D.B., Risingsong R., Williams C.R., Maitra A., Hruban R.H., Sporn M.B. Synthetic triterpenoids prolong survival in a transgenic mouse model of pancreatic cancer. Cancer Prev. Res. (Phila) 2010, 3:1427-1434.
24. Deeb D., Gao X., Liu Y., Jiang D., Divine G.W., Arbab A.S., Dulchavsky S.A., Gautam S.C. Synthetic triterpenoid CDDO prevents the progression and metastasis of prostate cancer in TRAMP mice by inhibiting survival signaling. Carcinogenesis 2011, 32:757-764.
25. Gao X., Deeb D., Liu Y., Arbab A.S., Divine G.W., Dulchavsky S.A., Gautam S.C. Prevention of prostate cancer with oleanane synthetic triterpenoid CDDO-Me in the tramp mouse model of prostate cancer. Cancers (Basel) 2011, 3:3353-3369.
26. Smith L.L., Coller H.A., Roberts J.M. Telomerase modulates expression of growth-controlling genes and enhances cell proliferation. Nat. Cell Biol. 2003, 5:474-479.
27. Herbert B., Pitts A.E., Baker S.I., Hamilton S.E., Wright W.E., Shay J.W., Corey D.R. Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proc. Natl. Acad. Sci. USA 1999, 96:14276-14281.
28. Zhang X., Mar V., Zhou W., Harrington L., Robinson M.O. Telomere shortening and apoptosis in telomerase-inhibited human tumor cells. Genes Dev. 1999, 13:2388-2399.
29. Park T.W., Riethdorf S., Riethdorf L., Loning T., Janicke F. Differential telomerase activity, expression of the telomerase catalytic sub-unit and telomerase-RNA in ovarian tumors. Int. J. Cancer 1999, 84:426-431.
30. Meeran S.M., Patel S.N., Tollefsbol T.O. Sulforaphane causes epigenetic repression of hTERT expression in human breast cancer cell lines. PLoS One 2010, 5:e11457.
31. Jagadeesh S., Kyo S., Banerjee P.P. Genistein represses telomerase activity via both transcriptional and posttranslational mechanisms in human prostate cancer cells. Cancer Res. 2006, 66:2107-2115.
32. Rabi T., Banerjee S. Novel semisynthetic triterpenoid AMR-Me inhibits telomerase activity in human leukemic CEM cells and exhibits in vivo antitumor activity against Dalton's lymphoma ascites tumor. Cancer Lett. 2009, 278:156-163.
33. Kyo S., Takakura M., Taira T., Kanaya T., Itoh H., Yutsudo M., Ariga H., Inoue M. Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT). Nucleic Acids Res. 2000, 28:669-677.
34. Konnikova L., Simeone M.C., Kruger M.M., Kotecki M., Cochran B.H. Signal transducer and activator of transcription 3 (STAT3) regulates human telomerase reverse transcriptase (hTERT) expression in human cancer and primary cells. Cancer Res. 2005, 65:6516-6520.
35. Kimura A., Ohmichi M., Kawagoe J., Kyo S., Mabuchi S., Takahashi T., Ohshima C., Arimoto-Ishida E., Nishio Y., Inoue M., Kurachi H., Tasaka K., Murata Y. Induction of hTERT expression and phosphorylation by estrogen via Akt cascade in human ovarian cancer cell lines. Oncogene 2004, 23:4505-4515.
36. Kang S.S., Kwon T., Kwon D.Y., Do S.I. Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase subunit. J. Biol. Chem. 1999, 274:13085-13090.
37. Liu Y., Gao X., Deeb D., Gautam S.C. Oleanane triterpenoid CDDO-Me inhibits Akt activity without affecting PDK1 kinase or PP2A phosphatase activity in cancer cells. Biochem. Biophys. Res. Commun. 2012, 417:570-575.
38. Li Y., Liu L., Andrews L.G., Tollefsbol T.O. Genistein depletes telomerase activity through cross-talk between genetic and epigenetic mechanisms. Int. J. Cancer 2009, 125:286-296.