MEHP-induced oxidative DNA damage and apoptosis in HepG2 cells correlates with p53-mediated mitochondria-dependent signaling pathway

Food and Chemical Toxicology

Volumn 50, Issue 7, 2012, Pages 2424-2431

  Yang, Guangtao ^a   Zhou, Xue ^a   Wang, Jing ^a   Zhang, Wenjuan ^a   Zheng, Hongyan ^a   Lu, Wenhong ^a   Yuan, Jing ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Apoptosis; Mitochondrial dysfunction; Mono (2 ethylhexyl)phthalate; Oxidative stress; P53

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; ADENOSINE TRIPHOSPHATE; ASCORBIC ACID; BETA ACTIN; CASPASE 3; CASPASE 9; GLUTATHIONE PEROXIDASE; MALONALDEHYDE; PHTHALIC ACID BIS(2 ETHYLHEXYL) ESTER; PROTEIN BAX; PROTEIN BCL 2; PROTEIN NOXA; PROTEIN P53; PUMA PROTEIN; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE;

APOPTOSIS; ARTICLE; CELL STRAIN HEPG2; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; CYTOTOXICITY TEST; DNA DAMAGE; DOWN REGULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; INTRACELLULAR SIGNALING; LIVER CELL CARCINOMA; LIVER TOXICITY; OXIDATIVE STRESS; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; UPREGULATION; WESTERN BLOTTING;

APOPTOSIS; CASPASE 3; CASPASE 9; CELL LINE, TUMOR; DIETHYLHEXYL PHTHALATE; DNA DAMAGE; FLOW CYTOMETRY; HUMANS; IN SITU NICK-END LABELING; MITOCHONDRIA; OXIDATIVE STRESS; SIGNAL TRANSDUCTION; TUMOR SUPPRESSOR PROTEIN P53;

EID: 84861438867     PISSN: 02786915     EISSN: 18736351     Source Type: Journal    
DOI: 10.1016/j.fct.2012.04.023     Document Type: Article

References (45)

1. Abdel-Hafez H.Z., Mohamed E.E., Abd-Elghany A.A. Tissue and blood superoxide dismutase activity and malondialdehyde level in leprosy. J. Eur. Acad. Dermatol. Venereol. 2010, 24:704-708.
2. Bissonnette S.L., Teague J.E., Sherr D.H., Schlezinger J.J. An endogenous prostaglandin enhances environmental phthalate-induced apoptosis in bone marrow B cells: activation of distinct but overlapping pathways. J. Immunol. 2008, 181:1728-1736.
3. Briganti S., Picardo M. Antioxidant activity, lipid peroxidation and skin diseases. What's new. J. Eur. Acad. Dermatol. Venereol. 2003, 17:663-669.
4. Chen T., Yang W., Li Y., Chen X., Xu S. Mono-(2-ethylhexyl) phthalate impairs neurodevelopment: inhibition of proliferation and promotion of differentiation in PC12 cells. Toxicol. Lett. 2011, 201:34-41.
5. Cheng M.L., Ho H.Y., Huang Y.W., Lu F.J., Chiu D.T. Humic acid induces oxidative DNA damage, growth retardation, and apoptosis in human primary fibroblasts. Exp. Biol. Med. (Maywood) 2003, 228:413-423.
6. Erkekoglu P., Rachidi W., Yuzugullu O.G., Giray B., Favier A., Ozturk M., Hincal F. Evaluation of cytotoxicity and oxidative DNA damaging effects of di(2-ethylhexyl)-phthalate (DEHP) and mono(2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium. Toxicol. Appl. Pharmacol. 2010, 248:52-62.
7. Erkekoglu P., Rachidi W., Yuzugullu O.G., Giray B., Ozturk M., Favier A., Hincal F. Induction of ROS, p53, p21 in DEHP- and MEHP-exposed LNCaP cells-protection by selenium compounds. Food Chem. Toxicol. 2011, 49:1565-1571.
8. Erkekoglu P., Zeybek N.D., Giray B., Asan E., Arnaud J., Hincal F. Reproductive toxicity of di(2-ethylhexyl) phthalate in selenium-supplemented and selenium-deficient rats. Drug Chem. Toxicol. 2011, 10.3109/01480545.2010.547499.
9. Fay M., Donohue J.M., De Rosa C. ATSDR evaluation of health effects of chemicals. VI. Di(2-ethylhexyl)phthalate. Agency for toxic substances and disease registry. Toxicol. Ind. Health 1999, 15:651-746.
10. Gross A., McDonnell J.M., Korsmeyer S.J. BCL-2 family members and the mitochondria in apoptosis. Genes Dev. 1999, 13:1899-1911.
11. Hainaut P., Milner J. Redox modulation of p53 conformation and sequence-specific DNA binding in vitro. Cancer Res. 1993, 53:4469-4473.
12. Halden R.U. Plastics and health risks. Annu. Rev. Public Health 2010, 31:179-194.
13. Heudorf U., Mersch-Sundermann V., Angerer J. Phthalates: toxicology and exposure. Int. J. Hyg. Environ. Health 2007, 210:623-634.
14. Ito Y., Yamanoshita O., Asaeda N., Tagawa Y., Lee C.H., Aoyama T., Ichihara G., Furuhashi K., Kamijima M., Gonzalez F.J., Nakajima T. Di(2-ethylhexyl)phthalate induces hepatic tumorigenesis through a peroxisome proliferator-activated receptor alpha-independent pathway. J. Occup. Health 2007, 49:172-182.
15. Iwasa F., Galbraith R.A., Sassa S. Effects of dimethyl sulphoxide on the synthesis of plasma proteins in the human hepatoma HepG2. Induction of an acute-phase-like reaction. Biochem. J. 1988, 253:927-930.
16. Kasahara E., Sato E.F., Miyoshi M., Konaka R., Hiramoto K., Sasaki J., Tokuda M., Nakano Y., Inoue M. Role of oxidative stress in germ cell apoptosis induced by di(2-ethylhexyl)phthalate. Biochem. J. 2002, 365:849-856.
17. Koniecki D., Wang R., Moody R.P., Zhu J. Phthalates in cosmetic and personal care products: concentrations and possible dermal exposure. Environ. Res. 2011, 111:329-336.
18. Kruse J.P., Gu W. Modes of p53 regulation. Cell 2009, 137:609-622.
19. Latini G., De Felice C., Presta G., Del Vecchio A., Paris I., Ruggieri F., Mazzeo P. Exposure to Di(2-ethylhexyl)phthalate in humans during pregnancy. A preliminary report. Biol. Neonate. 2003, 83:22-24.
20. Li N., Jia X., Chen C.Y., Blumberg J.B., Song Y., Zhang W., Zhang X., Ma G., Chen J. Almond consumption reduces oxidative DNA damage and lipid peroxidation in male smokers. J. Nutr. 2007, 137:2717-2722.
21. Lin L., Zheng L.X., Gu Y.P., Wang J.Y., Zhang Y.H., Song W.M. Levels of environmental endocrine disruptors in umbilical cord blood and maternal blood of low-birth-weight infants. Zhonghua Yu Fang Yi Xue Za Zhi 2008, 42:177-180.
22. Liu L., Bao H., Liu F., Zhang J., Shen H. Phthalates exposure of Chinese reproductive age couples and its effect on male semen quality, a primary study. Environ. Int. 2011, 10.1016/j.envint.2011.04.005.
23. Ma Q., Fang H., Shang W., Liu L., Xu Z., Ye T., Wang X., Zheng M., Chen Q., Cheng H. Superoxide flashes: early mitochondrial signals for oxidative stress-induced apoptosis. J. Biol. Chem. 2011, 286:27573-27581.
24. Mates J.M., Sanchez-Jimenez F. Antioxidant enzymes and their implications in pathophysiologic processes. Front. Biosci. 1999, 4:D339-D345.
25. Nakajima W., Tanaka N. Synergistic induction of apoptosis by p53-inducible Bcl-2 family proteins Noxa and Puma. J. Nihon. Med. Sch. 2007, 74:148-157.
26. Pabon A., Carmona J., Burgos L.C., Blair S. Oxidative stress in patients with non-complicated malaria. Clin. Biochem. 2003, 36:71-78.
27. Palmer C.N., Hsu M.H., Griffin K.J., Raucy J.L., Johnson E.F. Peroxisome proliferator activated receptor-alpha expression in human liver. Mol. Pharmacol. 1998, 53:14-22.
28. Rakkestad K.E., Holme J.A., Paulsen R.E., Schwarze P.E., Becher R. Mono(2-ethylhexyl) phthalate induces both pro- and anti-inflammatory responses in rat alveolar macrophages through crosstalk between p38, the lipoxygenase pathway and PPAR alpha. Inhal. Toxicol. 2010, 22:140-150.
29. Reinsberg J., Wegener-Toper P., van der Ven K., van der Ven H., Klingmueller D. Effect of mono-(2-ethylhexyl) phthalate on steroid production of human granulosa cells. Toxicol. Appl. Pharmacol. 2009, 239:116-123.
30. Rusyn I., Peters J.M., Cunningham M.L. Modes of action and species-specific effects of di-(2-ethylhexyl)phthalate in the liver. Crit. Rev. Toxicol. 2006, 36:459-479.
31. Shea K.M. Pediatric exposure and potential toxicity of phthalate plasticizers. Pediatrics 2003, 111:1467-1474.
32. Sjoberg P.O., Bondesson U.G., Sedin E.G., Gustafsson J.P. Exposure of newborn infants to plasticizers. Plasma levels of di-(2-ethylhexyl) phthalate and mono-(2-ethylhexyl) phthalate during exchange transfusion. Transfusion 1985, 25:424-428.
33. Svensson K., Hernandez-Ramirez R.U., Burguete-Garcia A., Cebrian M.E., Calafat A.M., Needham L.L., Claudio L., Lopez-Carrillo L. Phthalate exposure associated with self-reported diabetes among Mexican women. Environ. Res. 2011, 111:792-796.
34. Tsujimoto Y. Cell death regulation by the Bcl-2 protein family in the mitochondria. J. Cell Physiol. 2003, 195:158-167.
35. Urso M.L., Clarkson P.M. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003, 189:41-54.
36. Uzzaman M., Keller G., Germano I.M. Enhanced proapoptotic effects of tumor necrosis factor-related apoptosis-inducing ligand on temozolomide-resistant glioma cells. J. Neurosurg. 2007, 106:646-651.
37. Wang Q., Frolova A.I., Purcell S., Adastra K., Schoeller E., Chi M.M., Schedl T., Moley K.H. Mitochondrial dysfunction and apoptosis in cumulus cells of type I diabetic mice. PLoS One 2010, 5:e15901.
38. Wang X. The expanding role of mitochondria in apoptosis. Genes Dev. 2001, 15:2922-2933.
39. Wei M.C., Zong W.X., Cheng E.H., Lindsten T., Panoutsakopoulou V., Ross A.J., Roth K.A., MacGregor G.R., Thompson C.B., Korsmeyer S.J. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 2001, 292:727-730.
40. Xu Y., Cook T.J., Knipp G.T. Effects of di-(2-ethylhexyl)-phthalate (DEHP) and its metabolites on fatty acid homeostasis regulating proteins in rat placental HRP-1 trophoblast cells. Toxicol. Sci. 2005, 84:287-300.
41. Yang M.S., Gupta R.C. Determination of energy charge potential in the C6 glioma and the HepG-2 Cell Culture. Toxicol. Mech. Methods 2003, 13:97-101.
42. Yoshioka Y., Kitao T., Kishino T., Yamamuro A., Maeda S. Nitric oxide protects macrophages from hydrogen peroxide-induced apoptosis by inducing the formation of catalase. J. Immunol. 2006, 176:4675-4681.
43. Yu J., Zhang L. The transcriptional targets of p53 in apoptosis control. Biochem. Biophys. Res. Commun. 2005, 331:851-858.
44. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001, 7:673-682.
45. Yuan J., Lu W.Q., Zou Y.L., Wei W., Zhang C., Xie H., Chen X.M. Influence of aroclor 1254 on benzo(a)pyrene-induced DNA breakage, oxidative DNA damage, and cytochrome P4501A activity in human hepatoma cell line. Environ. Toxicol. 2009, 24:327-333.