A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay

Protein Science

Volumn 16, Issue 7, 2007, Pages 1422-1428

  Gutmann, Daniel A P ^a   Mizohata, Eiichi ^a   Newstead, Simon ^a   Ferrandon, Sebastian ^a,d   Henderson, Peter J F ^b   Van Veen, Hendrik W ^c   Byrne, Bernadette ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF LEEDS   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Aggregation; Detergent; High throughput; Membrane proteins; Monodispersity; Stability; Ultracentrifugation

Indexed keywords

BUFFER; DETERGENT; MEMBRANE PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; GEL PERMEATION CHROMATOGRAPHY; INTERMETHOD COMPARISON; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN STRUCTURE; SEDIMENTATION; SOLUBILITY; ULTRACENTRIFUGATION;

CHROMATOGRAPHY, GEL; CRYSTALLOGRAPHY, X-RAY; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MEMBRANE PROTEINS; SOLUBILITY; ULTRACENTRIFUGATION;

PROKARYOTA;

EID: 34250833480     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.072759907     Document Type: Article

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