Development and validation of a fluorescence-based hts assay for the identification of P/Q-type calcium channel blockers

Combinatorial Chemistry and High Throughput Screening

Volumn 15, Issue 5, 2012, Pages 372-385

  Mezler, Mario ^a   Hermann, David ^a   Swensen, Andrew M ^b   Draguhn, Andreas ^c   Terstappen, Georg C ^a   Gross, Gerhard ^a   Schoemaker, Hans ^a   Freiberg, Gail ^b   Pratt, Steve ^b   Gopalakrishnan, Sujatha M ^b   Nimmrich, Volker ^a  

^a ABBOTT GMBH AND CO KG   (Germany)

^b ABBOTT LABORATORIES   (United States)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Automated patch clamp; Electrophysiology; FLIPR; High throughput screening; P Q type voltage gated calcium channel; Tetracycline dependent expression

Indexed keywords

CALCIUM CHANNEL BLOCKING AGENT; CALCIUM CHANNEL P TYPE; CALCIUM CHANNEL Q TYPE; TETRACYCLINE;

ARTICLE; AUTOMATION; DRUG IDENTIFICATION; DRUG SCREENING; ELECTROPHYSIOLOGY; FLUORESCENCE; HIGH THROUGHPUT SCREENING; IC 50; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; VALIDATION STUDY;

CALCIUM CHANNEL BLOCKERS; CALCIUM CHANNELS, P-TYPE; CALCIUM CHANNELS, Q-TYPE; CELL LINE; ELECTROPHYSIOLOGY; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; PATCH-CLAMP TECHNIQUES; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

EID: 84860574611     PISSN: 13862073     EISSN: 18755402     Source Type: Journal    
DOI: 10.2174/138620712800194503     Document Type: Article

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