Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 3, 2012, Pages 431-441

  Kitova, Elena N ^a   El Hawiet, Amr ^a   Schnier, Paul D ^b   Klassen, John S ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b AMGEN INC   (United States)

Author keywords

Association constant; Binding assay; Electrospray ionization mass spectrometry; Library screening; Protein ligand interaction

Indexed keywords

ASSOCIATION CONSTANT; BINDING ASSAYS; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; LIBRARY SCREENING; PROTEIN-LIGAND INTERACTIONS;

CARBOHYDRATES; DIAGNOSIS; MASS SPECTROMETRY; STOICHIOMETRY;

ASSOCIATION REACTIONS;

ANALYTICAL ERROR; ARTICLE; BINDING AFFINITY; BINDING ASSAY; COLLISIONALLY ACTIVATED DISSOCIATION; COMPLEX FORMATION; CONCENTRATION RESPONSE; ELECTROSPRAY MASS SPECTROMETRY; LIGAND BINDING; PH; PROTEIN AGGREGATION; PROTEIN INTERACTION; RELIABILITY; SENSITIVITY ANALYSIS; STOICHIOMETRY; SURFACE PROPERTY; TEMPERATURE SENSITIVITY; CHEMISTRY; METABOLISM; METHODOLOGY; PROTEIN BINDING; PROTEIN DATABASE; TEMPERATURE;

LIGAND; PROTEIN;

DATABASES, PROTEIN; HYDROGEN-ION CONCENTRATION; LIGANDS; PROTEIN BINDING; PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TEMPERATURE;

EID: 84860557228     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0311-9     Document Type: Article

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