Optimization of antibody fragment production in Bacillus megaterium: The role of metal ions on protein secretion

Journal of Biotechnology

Volumn 150, Issue 1, 2010, Pages 115-124

  David, F ^a   Westphal, R ^a   Bunk, B ^a   Jahn, D ^a   Franco Lara, E ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Genetic algorithm; Microtiter plates; Multi objective optimization; Recombinant protein

Indexed keywords

ANTIBODY FRAGMENT; BACILLUS MEGATERIUM; DEEP WELLS; KEY COMPONENT; KEY ELEMENTS; MEDIA COMPOSITION; METAL ION CONCENTRATION; MICROTITER PLATES; MINIMAL MEDIAS; PARETO-FRONT; PROTEIN PRODUCTION; PROTEIN SECRETION; RECOMBINANT PROTEIN;

ANTIBODIES; BACTERIOLOGY; BIOMASS; GENETIC ALGORITHMS; MAGNESIUM; METAL IONS; METALS;

MULTIOBJECTIVE OPTIMIZATION;

ANTIBODY; COBALT; CUPRIC ION; FERRIC ION; FRUCTOSE; MAGNESIUM; MANGANESE; METAL ION; ZINC ION;

ANTIBODY PRODUCTION; ARTICLE; BACILLUS MEGATERIUM; BIOMASS; GENETIC ALGORITHM; GENOME; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; OXYGEN CONCENTRATION; PLASMID; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN SECRETION; SCREENING;

ALGORITHMS; BACILLUS MEGATERIUM; BIOMASS; BIOREACTORS; COMPUTATIONAL BIOLOGY; CULTURE MEDIA; ENZYME-LINKED IMMUNOSORBENT ASSAY; FRUCTOSE; HUMANS; INTRACELLULAR SPACE; MAGNESIUM; METALS, HEAVY; MODELS, CHEMICAL; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

BACILLUS MEGATERIUM;

EID: 77957364733     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.07.023     Document Type: Article

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