Influence of fructose and oxygen gradients on fed-batch recombinant protein production using Bacillus megaterium

Engineering in Life Sciences

Volumn 11, Issue 4, 2011, Pages 338-349

  Korneli, Claudia ^a   David, Florian ^a   Godard, Thibault ^a   Franco Lara, Ezequiel ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

DO based feeding; Flow cytometry; Scale down; Single cell analysis; Substrate gradients

Indexed keywords

BACILLUS MEGATERIUM; CONSTANT GRADIENTS; CONTROL PARAMETERS; CONTROLLER OUTPUTS; DO CONCENTRATION; DO-BASED FEEDING; EXPERIMENTAL APPROACHES; FED BATCHES; FEED CONTROL; GREEN FLUORESCENT PROTEIN; HIGH FREQUENCY OSCILLATIONS; LOW-AMPLITUDE; OPTIMAL FEEDING; PRODUCT FORMATION; PUMPING CAPACITY; RECOMBINANT PROTEIN PRODUCTIONS; SCALE DOWN; SINGLECELL ANALYSIS; STIRRED TANK REACTORS; SUBSTRATE CONCENTRATIONS;

BACILLI; BACTERIOLOGY; CONTROLLERS; FEEDING; FLOW CYTOMETRY; FRUCTOSE; OPTIMIZATION; PROTEINS; STICTION; SUBSTRATES;

DISSOLVED OXYGEN;

ALKALINE PROTEINASE; CARBOXYLIC ACID; DISSOLVED OXYGEN; FRUCTOSE; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; XYLOSE;

BACTERIUM; BIOMASS; BIOREACTOR; DISSOLVED OXYGEN; EXPERIMENTAL STUDY; FLOW CYTOMETRY; OXYGEN; PUMPING; SUBSTRATE; SUGAR;

ARTICLE; BACILLUS MEGATERIUM; BACTERIAL STRAIN; BIOMASS; BIOREACTOR; CELL GROWTH; CELL METABOLISM; CELL SUSPENSION; FLOW CYTOMETRY; FLOW RATE; HIGH FREQUENCY OSCILLATION; NONHUMAN; OPTICAL DENSITY; OSCILLATION; PROTEIN SYNTHESIS; SINGLE CELL ANALYSIS; SPECTROMETRY;

BACILLUS MEGATERIUM;

EID: 84860389633     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201000161     Document Type: Article

References (36)

1. Vary, P. S., Biedendieck, R., Fürch, T., Meinhardt, et al., Bacillus megaterium -- from simple soil bacterium to industrial protein production host. Appl. Microbiol. Biotechnol. 2007, 76, 957-967.
2. Biedendieck, R., Beine, R., Gamer, M., Jordan, E., et al., Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. Appl. Microbiol. Biotechnol. 2007, 74, 1062-1073.
3. Martin, L., Prieto, M. A., Cortes, E., Garcia, J. L., Cloning and sequencing of the pac gene encoding the penicillin G acylase of Bacillus megaterium ATCC 14945. FEMS Microbiol. Lett. 1995, 125, 287-292.
4. Takasaki, Y., Novel maltose-producing amylase from Bacillus megaterium G-2. Agri. Biol. Chem. 1989, 53, 341-347.
5. Raux, E., Lanois, A., Warren, M. J., Rambach, A., Thermes, C., Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon. Biochem. J. 1998, 335, 159-166.
6. He, J. S., Fulco, A. J., A barbiturate-regulated protein binding to a common sequence in the cytochrome P450 genes of rodents and bacteria. J. Biol. Chem. 1991, 266, 7864-7869.
7. Wang, W., Hollmann, R., Furch, T., Nimtz, M., et al., Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase. Proteome Sci. 2005, 3, 4.
8. Furch, T., Wittmann, C., Wang, W., Franco-Lara, E., et al., Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. J. Biotechnol. 2007, 132, 385-394.
9. Moes, J., Griot, M., Keller, J., Heinzle, E., et al., A microbial culture with oxygen-sensitive product distribution as a potential tool for characterizing bioreactor oxygen transport. Biotechnol. Bioeng. 1985, 27, 482-489.
10. Hollmann, R., Deckwer, W. D., Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation. J. Biotechnol. 2004, 111, 89-96.
11. Biedendieck, R., Yang, Y., Deckwer, W. D., Malten, M., Jahn, D., Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium. Biotechnol. Bioeng. 2007, 96, 525-537.
12. Cutayar, J. M., Poillon, D., High cell density culture of E. coli in a fed-batch system with dissolved oxygen as substrate feed indicator. Biotechnol. Lett. 1989, 11, 155-160.
13. Stammen, S., Müller, B. K., Korneli, C., Biedendieck, R., et al., High-yield intra- and extracellular protein production using Bacillus megaterium. Appl. Environ. Microbiol. 2010, 76, 4037-4046.
14. Enfors, S. O., Jahic, M., Rozkov, A., Xu, B., et al., Physiological responses to mixing in large scale bioreactors. J. Biotechnol. 2001, 85, 175-185.
15. Bylund, F., Collet, E., Enfors, S. O., Larsson, G., Substrate gradient formation in the large-scale bioreactor lowers cell yield and increases by-product formation. Bioproc. Biosyst. Eng. 1998, 18, 171-180.
16. George, S., Larsson, G., Olsson, K., Enfors, S. O., Comparison of the Baker's yeast process performance in laboratory and production scale. Bioprocess. Eng. 1998, 18, 135-142.
17. Delvigne, F., Boxus, M., Ingels, S., Thonart, P., Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coli. Microb. Cell Fact. 2009, 8, 15.
18. Hewitt, C. J., Onyeaka, H., Lewis, G., Taylor, I. W., Nienow, A. W., A comparison of high cell density fed-batch fermentations involving both induced and non-induced recombinant Escherichia coli under well-mixed small-scale and simulated poorly mixed large-scale conditions. Biotechnol. Bioeng. 2007, 96, 495-505.
19. Lara, A. R., Galindo, E., Ramirez, O. T., Palomares, L. A., Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells. Mol. Biotechnol. 2006, 34, 355-381.
20. Amanullah, A., McFarlane, C. M., Emery, A. N., Nienow, A. W., Scale-down model to simulate spatial pH variations in large-scale bioreactors. Biotechnol. Bioeng. 2001, 73, 390-399.
21. Biedendieck, R., Yang, Y., Deckwer, W.-D., Malten, M., Jahn, D., Plasmid System for the Intracellular Production and Purification of Affinity-Tagged Proteins in Bacillus megaterium. Biotechnol. Bioeng. 2007, 96, 525-537.
22. Yang, Y., Biedendieck, R., Wang, W., Gamer, M., et al., High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium. Microb. Cell Fact. 2006, 5, 36.
23. Wittchen, K. D., Meinhardt, F., Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl. Microbiol. Biotechnol. 1995, 42, 871-877.
24. Rygus, T., Hillen, W., Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl. Microbiol. Biotechnol. 1991, 35, 594-599.
25. Amanullah, A., Nienow, A. W., Emery, A. N., McFarlane, C. M., The use of Bacillus subtilis as an oxygen sensitive culture to simulate dissolved oxygen cycling in large scale fermenters. Food Bioprod. Process.: Trans. Inst. Chem. Eng., Part C 1993, 71, 206-208.
26. Lüders, S., David, F., Steinwand, M., Jordan, E., et al., Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Appl. Microbiol. Biotechnol. 2011, doi: 10.1007/s00253-011-3193-7.
27. Yoon, S. M., Kim, S. C., Kim, J. H., Identification of inhibitory metabolites in high density culture of recombinant Bacillus megaterium pCK108. Biotechnol. Lett. 1994, 16, 1011-1014.
28. Lara, A. R., Leal, L., Flores, N., Gosset, G., et al., Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system. Biotechnol. Bioeng. 2006, 93, 372-385.
29. Salmon, K., Hung, S. P., Mekjian, K., Baldi, P., et al., Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR. J. Biol. Chem. 2003, 278, 29837-29855.
30. Lüders, S., Prozess- und Proteomanalyse gestresster Mikroorganismen. Cuvillier-Verlag, Göttingen 2010. ISBN 1431-7230.
31. Schweder, T., Kruger, E., Xu, B., Jurgen, B., et al., Monitoring of genes that respond to process-related stress in large-scale bioprocesses. Biotechnol. Bioeng. 1999, 65, 151-159.
32. Lüders, S., Fallet, C., Franco-Lara, E., Proteome analysis of the Escherichia coli heat shock response under steady-state conditions. Proteome Sci. 2009, 7, 36.
33. Yang, F., Moss, L. G., Phillips, G. N., Jr., The molecular structure of green fluorescent protein. Nat. Biotechnol. 1996, 14, 1246-1251.
34. Takahashi, E., Takano, T., Numata, A., Hayashi, N., et al., Genetic oxygen sensor: GFP as an indicator of intracellular oxygenation. Adv. Exp. Med. Biol. 2005, 566, 39-44.
35. Cormack, B. P., Valdivia, R. H., Falkow, S., FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173, 33-38.
36. Gnoth, S., Jenzsch, M., Simutis, R., Lubbert, A., Control of cultivation processes for recombinant protein production: a review. Bioprocess Biosyst. Eng. 2008, 31, 21-39.