Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

BioTechniques

Volumn 52, Issue 4, 2012, Pages 263-270

  Feng, Lin ^a   Lintula, Susanna ^b   Ho, Tho Huu ^a   Anastasina, Maria ^b   Paju, Annukka ^c   Haglund, Caj ^c   Stenman, Ulf Håkan ^b   Hotakainen, Kristina ^b,c   Orpana, Arto ^b,c   Kainov, Denis ^b   Stenman, Jakob ^a,b,d  

^a MINERVA FOUNDATION INSTITUTE FOR MEDICAL RESEARCH   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

^d KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Primer independent reverse transcription; Proportion; RT PCR

Indexed keywords

COMPLEMENTARY DNA; PRIMER DNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; DNA SYNTHESIS; GENE AMPLIFICATION; GENE EXPRESSION; GENE TARGETING; INFLUENZA VIRUS A; NONHUMAN; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SYNTHESIS; TEMPERATURE;

ANIMALS; BASE SEQUENCE; CELL LINE, TUMOR; DNA PRIMERS; DNA, COMPLEMENTARY; DOGS; GENE EXPRESSION REGULATION; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID DENATURATION; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA REPLICASE; RNA, VIRAL; TEMPERATURE; VIRAL PROTEINS;

EUKARYOTA; INFLUENZA A VIRUS; RNA VIRUSES;

EID: 84859467162     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/0000113842     Document Type: Article

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