Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters

Science

Volumn 322, Issue 5909, 2008, Pages 1845-1848

  Core, Leighton J ^a   Waterfall, Joshua J ^a   Lis, John T ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; RNA POLYMERASE;

ENZYME ACTIVITY; GENE EXPRESSION; GENOME; HOMINID; RNA;

ARTICLE; GENETIC VARIABILITY; HUMAN; NUCLEOSOME; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; RNA CLEAVAGE; RNA SEQUENCE; RNA TRANSCRIPTION; TRANSCRIPTION REGULATION;

CELL LINE; CPG ISLANDS; DNA-DIRECTED RNA POLYMERASES; GENOME, HUMAN; HUMANS; NUCLEOSOMES; PROMOTER REGIONS, GENETIC; RNA POLYMERASE II; RNA, ANTISENSE; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC;

EID: 57849109058     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1162228     Document Type: Article

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24. We gratefully thank C. Haudenschild for advice on construction of our libraries and for performing the initial alignments, Q. Sun and L. Ponnala for aligning the trimmed reads, A. Siepel for computational and statistical discussion, and the members of the Lis lab for suggestions regarding this work. The work was funded by NIH grant GM25232 to J.T.L. The data discussed in this publication have been deposited in National Center for Biotechnology Information's Gene Expression Omnibus under accession number GSE13518. The authors are filing a patent based on the work in this paper.