Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA

Journal of Virological Methods

Volumn 173, Issue 1, 2011, Pages 1-6

  Kawakami, Eiryo ^a   Watanabe, Tokiko ^b   Fujii, Ken ^b   Goto, Hideo ^a   Watanabe, Shinji ^b   Noda, Takeshi ^a   Kawaoka, Yoshihiro ^a,b,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Detection of influenza vRNA, cRNA and mRNA; Influenza A virus; Quantitative real time PCR; Strand specific real time RT PCR

Indexed keywords

COMPLEMENTARY RNA; MESSENGER RNA; VIRUS RNA;

ANIMAL CELL; ARTICLE; GENE DOSAGE; INFLUENZA A; INFLUENZA VIRUS A; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SEQUENCE; RNA SYNTHESIS; STRAND SPECIFIC REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CELL LINE; DNA PRIMERS; DOGS; INFLUENZA A VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, COMPLEMENTARY; RNA, MESSENGER; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIROLOGY;

INFLUENZA A VIRUS; ORTHOMYXOVIRIDAE;

EID: 79952189030     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.12.014     Document Type: Article

References (18)

1. Bouvier N., Palese P. The biology of influenza viruses. Vaccine 2008, 26:D49-D53.
2. Craggs J., Ball J., Thomson B., Irving W., Grabowska A. Development of a strand-specific RT-PCR based assay to detect the replicative form of hepatitis C virus RNA. J. Virol. Methods 2001, 94:111-120.
3. Ge Q., McManus M.T., Nguyen T., Shen C.H., Sharp P.A., Eisen H.N., Chen J.Z. RNA interference of influenza virus production by directly targeting rnRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc. Natl. Acad. Sci. U.S.A. 2003, 100:2718-2723.
4. Hatada E., Hasegawa M., Mukaigawa J., Shimizu K., Fukuda R. Control of influenza virus gene expression: quantitative analysis of each viral RNA species in infected cells. J. Biochem. 1989, 105:537-546.
5. Karlas A., Machuy N., Shin Y., Pleissner K., Artarini A., Heuer D., Becker D., Khalil H., Ogilvie L., Hess S., Mäurer A., Müller E., Wolff T., Rudel T., Meyer T. Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication. Nature 2010, 463:818-822.
6. Lanford R., Sureau C., Jacob J., White R., Fuerst T. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 1994, 202:606-614.
7. Lee K., Seong B. The position 4 nucleotide at the 3′ end of the influenza virus neuraminidase vRNA is involved in temporal regulation of transcription and replication of neuraminidase RNAs and affects the repertoire of influenza virus surface antigens. J. Gen. Virol. 1998, 79:1923-1934.
8. Mackay I., Arden K., Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 2002, 30:1292-1305.
9. Mizuno Y., Carninci P., Okazaki Y., Tateno M., Kawai J., Amanuma H., Muramatsu M., Hayashizaki Y. Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display. Nucleic Acids Res. 1999, 27:1345-1349.
10. Neumann G., Brownlee G., Fodor E., Kawaoka Y. Orthomyxovirus replication, transcription, and polyadenylation. Curr. Top. Microbiol. Immunol. 2004, 283:121-144.
11. Neumann G., Watanabe T., Ito H., Watanabe S., Goto H., Gao P., Hughes M., Perez D., Donis R., Hoffmann E. Generation of influenza A viruses entirely from cloned cDNAs. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:9345-9350.
12. Palese P., Shaw M. Orthomyxoviridae: the viruses and their replication. Fields Virol 2007, 1647-1689. Lippincott Williams & Wilkins, Philadelphia. 5th ed. D. Knipe, P. Howley (Eds.).
13. Portela A., Digard P. The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J. Gen. Virol. 2002, 83:723-734.
14. Robb N., Smith M., Vreede F., Fodor E. NS2/NEP protein regulates transcription and replication of the influenza virus RNA genome. J. Gen. Virol. 2009, 90:1398-1407.
15. Shapiro G., Gurney T., Krug R. Influenza virus gene expression: control mechanisms at early and late times of infection and nuclear-cytoplasmic transport of virus-specific RNAs. J. Virol. 1987, 61:764-773.
16. Spackman E., Senne D., Myers T., Bulaga L., Garber L., Perdue M., Lohman K., Daum L., Suarez D. Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J. Clin. Microbiol. 2002, 40:3256-3260.
17. Vester D., Lagoda A., Hoffmann D., Seitz C., Heldt S., Bettenbrock K., Genzel Y., Reichl U. Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics. J. Virol. Methods 2010, 168:63-71.
18. Vreede F., Jung T., Brownlee G. Model suggesting that replication of influenza virus is regulated by stabilization of replicative intermediates. J. Virol. 2004, 78:9568-9572.