Development of a Taqman RT-PCR assay for the detection and quantification of negatively stranded RNA of human enteroviruses: Evidence for false-priming and improvement by tagged RT-PCR

Journal of Virological Methods

Volumn 153, Issue 2, 2008, Pages 182-189

  Bessaud, Maël ^a   Autret, Arnaud ^a   Jegouic, Sophie ^a   Balanant, Jean ^a   Joffret, Marie Line ^a   Delpeyroux, Francis ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Human enterovirus; Minus stranded RNA; Real time PCR; Replication; Silica based purification; Tagged RT PCR

Indexed keywords

SINGLE STRANDED RNA; VIRUS RNA;

AMPLICON; ARTICLE; CONTROLLED STUDY; ENTEROVIRUS; FALSE POSITIVE RESULT; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY ANALYSIS; VIRION; VIRUS INFECTION;

CELL LINE; DNA PRIMERS; DNA, COMPLEMENTARY; ENTEROVIRUS; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE; VIRUS REPLICATION;

ENTEROVIRUS; RNA VIRUSES;

EID: 53049094782     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.07.010     Document Type: Article

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