Real-time RT-qPCR assay for the analysis of human influenza A virus transcription and replication dynamics

Journal of Virological Methods

Volumn 168, Issue 1-2, 2010, Pages 63-71

  Vester, Diana ^a   Lagoda, Antje ^a   Hoffmann, Diana ^b   Seitz, Claudius ^c   Heldt, Stefan ^c   Bettenbrock, Katja ^c   Genzel, Yvonne ^c   Reichl, Udo ^a,c  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b TECHNISCHE HOCHSCHULE MITTELHESSEN   (Germany)

^c MAX PLANCK INSTITUTE FOR DYNAMICS OF COMPLEX TECHNICAL SYSTEMS   (Germany)

Author keywords

Influenza A virus; Polarity specific reverse transcription; Quantitative real time PCR; RNA reference standards; Virus replication dynamics

Indexed keywords

MESSENGER RNA; VIRUS RNA;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL CULTURE; CELL STRAIN; CONTROLLED STUDY; DYNAMICS; INFLUENZA VIRUS A; LIFE CYCLE; MAMMAL; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SYNTHESIS; SENSITIVITY ANALYSIS; VALIDATION PROCESS; VIRUS GENOME; VIRUS REPLICATION; VIRUS TRANSCRIPTION; ANIMAL; BIOSYNTHESIS; DOG; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; PHYSIOLOGY; PROCEDURES; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

INFLUENZA A VIRUS; MAMMALIA; ORTHOMYXOVIRIDAE;

ANIMALS; CELLS, CULTURED; DOGS; GENE EXPRESSION PROFILING; INFLUENZA A VIRUS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TRANSCRIPTION, GENETIC; VIRUS REPLICATION;

EID: 77955094012     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.04.017     Document Type: Article

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