Commercial reverse transcriptase as source of false-positive strand-specific RNA detection in human cells

Biochimie

Volumn 93, Issue 10, 2011, Pages 1731-1737

  Moison, Celine ^a,b,c   Arimondo, Paola B ^a,b,d   Guieysse Peugeot, Anne Laure ^a,b  

^a MUSÉUM NATIONAL D'HISTOIRE NATURELLE   (France)

^b INSERM   (France)

^c SORBONNE UNIVERSITÉ   (France)

^d INSTITUT DE RECHERCHE PIERRE FABRE   (France)

Author keywords

ncRNA; RT false priming; RT PCR false positive; Strand specific RNA detection

Indexed keywords

COMPLEMENTARY DNA; COMPLEMENTARY RNA; PRIMER RNA; RETINOIC ACID RECEPTOR BETA; RETINOIC ACID RECEPTOR BETA 2; RNA DIRECTED DNA POLYMERASE; UNCLASSIFIED DRUG; UNTRANSLATED RNA;

ACCURACY; ARTICLE; CONTROLLED STUDY; DNA SYNTHESIS; FALSE POSITIVE RESULT; GENE AMPLIFICATION; HUMAN; HUMAN CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TEMPERATURE; TUMOR CELL LINE;

CELL LINE, TUMOR; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA-DIRECTED DNA POLYMERASE;

EID: 80052094817     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2011.06.005     Document Type: Article

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