Production and secretion of recombinant thaumatin in tobacco hairy root cultures

Biotechnology Journal

Volumn 7, Issue 4, 2012, Pages 537-545

  Pham, Ngoc Bich ^a,b   Schäfer, Holger ^b   Wink, Michael ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b INSTITUTE OF BIOTECHNOLOGY   (Viet Nam)

Author keywords

Agrobacterium rhizogenes; Hairy roots; Recombinant protein; Sweet tasting protein; Thaumatin

Indexed keywords

AGROBACTERIUM RHIZOGENES; CALRETICULIN; CULTURE MEDIUM; EXTRACELLULAR; HAIRY ROOT CULTURE; HAIRY ROOTS; ORGAN CULTURE; PLANT CELLS; POLY VINYL PYRROLIDONE; PROTEIN YIELD; PURIFICATION PROCEDURES; ROOT CELLS; SIGNAL PEPTIDE; STATIONARY PHASE; THAUMATIN; TRANSGENIC PLANTS;

ENZYME ACTIVITY; GENE ENCODING; PHYSIOLOGY; PLANT CELL CULTURE; PRODUCTION PLATFORMS; SODIUM CHLORIDE; TOBACCO;

RECOMBINANT PROTEINS;

CALRETICULIN; POVIDONE; PROTEINASE; SIGNAL PEPTIDE; SODIUM CHLORIDE; THAUMATIN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HAIRY ROOT CULTURE; NONHUMAN; PLANT CELL; PLANT ROOT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; TOBACCO; TRANSGENIC PLANT;

ENDOPLASMIC RETICULUM; PLANT PROTEINS; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; POVIDONE; RECOMBINANT PROTEINS; SODIUM CHLORIDE; TOBACCO;

AGROBACTERIUM RHIZOGENES; NICOTIANA TABACUM;

EID: 84859238209     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100430     Document Type: Article

References (50)

1. Doran, P. M., Foreign protein production in plant tissue cultures. Curr. Opin. Biotechnol. 2000, 11, 199-204.
2. Hellwig, S., Drossard, J., Twyman, R. M., Fischer, R., Plant cell cultures for the production of recombinant proteins. Nat. Biotechnol. 2004, 22, 1415-1422.
3. Evangelista, R. L., Kusnadi, A. R., Howard, J. A., Nikolov, Z. L., Process and economic evaluation of the extraction and purification of recombinant beta-glucuronidase from transgenic corn. Biotechnol. Progr. 1998, 14, 607-614.
4. Giri, A., Narasu, M. L., Transgenic hairy roots: recent trends and applications. Biotechnol. Adv. 2000, 18, 1-22.
5. Wink, M., The cell culture medium-a functional extracellular compartment of suspension cultured cells. Plant Cell Tissue Organ Cult. 1994, 38, 307-319.
6. Sharp, J. M., Doran, P. M., Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol. Prog. 2001, 17, 979-992.
7. Doran, P. M., Foreign protein degradation and instability in plants and plant tissue cultures. Trends Biotechnol. 2006, 24, 426-432.
8. van der Wel, H., Ledeboer, A. M., The thaumatins, in: Marcus, A. (Ed.), The Biochemistry of Plants-A Comprehensive Treatise, Academic Press, New York, 1989, pp. 379-391.
9. van der Wel, H., Loeve, K., Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii (Benth). Eur. J. Biochem. 1972, 31, 221-225.
10. Masuda, T., Kitabatake, N., Developments in biotechnological production of sweet proteins. J. Biosci. Bioeng. 2006, 102, 375-389.
11. Iyengar, R. B., Smits, P., van der Ouderaa, F. J. G., van der Wel, H. et al., The complete amino acid sequence of the sweet-tasting protein thaumatin I. Eur. J. Biochem. 1979, 96, 193-204.
12. Edens, L., Heslinga, L., Klok, R., Ledeboer, A. M. et al., Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene 1982, 18, 1-12.
13. Daniell, S., Mellits, K. H., Faus, I., Connerton, I., Refolding the sweet-tasting protein thaumatin II from insoluble inclusion bodies synthesised in Escherichia coli. Food Chem. 2000, 71, 105-110.
14. Faus, I., Patino, C., Rio, J. L., del Moral, C. et al., Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli. Biochem. Biophys. Res. Commun. 1996, 229, 121-127.
15. Illingworth, C., Larson, G., Hellekant, G., Secretion of the sweet-tasting plant protein thaumatin by Bacillus subtilis. Biotech. Lett. 1988, 10, 587-592.
16. Illingworth, C., Larson, G., Hellekant, G., Secretion of the sweet-tasting plant protein thaumatin by Streptomyces lividans. J. Ind. Microbiol. 1989, 4, 37-42.
17. Faus, I., del Moral, C., Adroer, N., del Rio, J. L. et al., Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori. Appl. Microbiol. Biotechnol. 1998, 49, 393-398.
18. Moralejo, F. J., Cardoza, R. E., Gutierrez, S., Martin, J. F., Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage. Appl. Environ. Microbiol. 1999, 65, 1168-1174.
19. Lee, J. H., Weickmann, J. L., Koduri, R. K., Ghosh-Dastidar, P. et al., Expression of synthetic thaumatin genes in yeast. Biochemistry 1988, 27, 5101-5107.
20. Masuda, T., Tamaki, S., Kaneko, R., Wada, R. et al., Cloning, expression and characterization of recombinant sweet-protein thaumatin II using the methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 2004, 85, 761-769.
21. Masuda, T., Ohta, K., Mikami, B., Kitabatake, N., High-resolution structure of the recombinant sweet-tasting protein thaumatin I. Acta Crystallogr. Sect. F 2011, 67, 652-658.
22. Witty, M., Thaumatin II: A simple marker gene for use in plants. Nucleic Acids Res. 1989, 17, 3312.
23. Bartoszewski, G., Niedziela, A., Szwacka, M., Niemirowicz-Szczytt, K., Modification of tomato taste in transgenic plants carrying a thaumatin gene from Thaumatococcus daniellii Benth. Plant Breed. 2003, 122, 347-351.
24. Szwacka, M., Siedlecka, E., Zawirska-Wojtasiak, R., Wisniewski, L., Malepszy, S., Expression pattern of the pre-prothaumatin II gene under the control of the CaMV 35S promoter in transgenic cucumber (Cucumis sativus L.) flower buds and fruits. J. Appl. Genet. 2009, 50, 9-16.
25. Schestibratov, K. A., Dolgov, S. V., Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea. Scientia Hortic. Amsterdam 2005, 106, 177-189.
26. Lebedev, V. G., Taran, S. A., Shmatchenko, V. V., Dolgov, S. V., Pear transformation with the gene for supersweet protein thaumatin II. Acta Hortic. 2002, 1, 199-202.
27. Stahl, R., Luhrs, R., Dargatz, H., Thaumatin from transgenic barley. US Patent Application 20090031458.
28. Pushin, A. S., Ovchinnikova, E. V., Shul'ga, O. A., Firsov, A. P., Dolgov, S. V., Accumulation of a supersweet protein thaumatin II in apoplast of tobacco transgenic plants. Biotekhnologiya 2008, 31-40.
29. Karimi, M., Inze, D., Depicker, A., GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 2002, 7, 193-195.
30. Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 1962, 15, 473-497.
31. Lloyd, G., McCown, B., Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Comb. Proc. Int. Plant Propagators Soc. 1981, 30, 421-437.
32. Khanuja, P. S. S., Shasany, A. K., Darokar, M. P., Kumar, S., Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant Mol. Biol. Rep. 1999, 17, 1-7.
33. Zayed, R., Wink, M., Induction of tropane alkaloid formation in transformed root cultures of Brugmansia suaveolens (Solanaceae). Z. Naturforsch. 2004, 59c, 863-867.
34. Richards, H. A., Halfhill, M. D., Millwood, R. J., Stewart, C. N. Jr., Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants. Plant Cell Rep. 2003, 22, 117-121.
35. Hakkinen, S. T., Moyano, E., Cusido, R. M., Palazon, J. et al., Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase. J. Exp. Bot. 2005, 56, 2611-2618.
36. Dotsikas, Y., Loukas, Y. L., Effect of the luminol signal enhancer selection on the curve parameters of an immunoassay and the chemiluminescence intensity and kinetics. Talanta 2007, 71, 906-910.
37. Wilkinson, B., Gilbert, H. F., Protein disulfide isomerase. Biochim. Biophys. Acta 2004, 1699, 35-44.
38. Conrad, U., Fiedler, U., Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity. Plant Mol. Biol. 1998, 38, 101-109.
39. Martnez, C., Petruccelli, S., Giulietti, A. M., Alvarez, M. A., Expression of the antibody 14D9 in Nicotiana tabacum hairy roots. Electron. J. Biotechnol. 2005, 8, 170-176.
40. Doran, P. M., Loss of secreted antibody from transgenic plant tissue cultures due to surface adsorption. J. Biotechnol. 2006, 122, 39-54.
41. Szwacka, M., Krzymowska, M., Osuch, A., Kowalczyk, M., Malepszy, S., Variable properties of transgenic cucumber plants containing the thaumatin II gene from Thaumatococcus daniellii. Acta Physiol. Plant 2002, 24, 173-185.
42. Ma, J. C., Drake, P. M. W., Christou, P., The production of recombinant pharmaceutical protein in plants. Nat. Rev. Genet. 2003, 4, 794-805.
43. Borisjuk, N. V., Borisjuk, L. G., Logendra, S., Petersen, F. et al., Production of recombinant proteins in plant root exudates. Nat. Biotechnol. 1999, 17, 466-469.
44. Grignon, C., Sentenac, H., pH and ionic conditions in the apoplast. Ann. Rev. Plant Physiol. Plant Mol. Biol. 1991, 42, 103-128.
45. Magnuson, N. S., Linzmaier, P. M., Gao, J. W., Reeves, R. et al., Enhanced recovery of a secreted mammalian protein from suspension culture of genetically modified tobacco cells. Protein Expression Purif. 1996, 7, 220-228.
46. LaCount, W., An, G. H., Lee, J. M., The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol. Lett. 1997, 19, 93-96.
47. James, E. A., Wang, C., Wang, Z., Reeves, R. et al., Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Expression Purif. 2000, 19, 131-138.
48. Lee, J. H., Kim, N. S., Kwon, T. H., Jang, Y. S., Yang, M. S., Increased production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the addition of stabilizing polymer in plant suspension cultures. J. Biotechnol. 2002, 96, 205-211.
49. Lee, J. H., Kim, N. S., Kwon, T. H., Yang, M. S., Effects of osmotic pressure on production of recombinant human granulocyte-macrophage colony stimulating factor in plant cell suspension culture. Enzyme Microb. Technol. 2002, 30, 768-773.
50. Shadwick, F. S., Doran, P. M., Foreign protein expression using plant cell suspension and hairy root cultures, in: Fischer, R., Schillberg, S. (Eds.), Molecular Farming-Plant-Made Pharmaceuticals and Technical Proteins, Wiley-VCH, Weinheim 2004, pp. 13-36.