Refolding the sweet-tasting protein thaumatin II from insoluble inclusion bodies synthesised in Escherichia coli

Food Chemistry

Volumn 71, Issue 1, 2000, Pages 105-110

  Daniell, Sarah ^a   Mellits, Kennneth H ^b   Faus, Ignacio ^c   Connerton, Ian ^a,b  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c URQUIMA SA Uriach Group   (Spain)

Author keywords

Protein refolding; Sweet taste; Thaumatin; Thiol disulfide redox

Indexed keywords

GLUTATHIONE; RECOMBINANT PROTEIN; THAUMATIN; THAUMATIN II; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; GENE EXPRESSION SYSTEM; HUMAN; MOUSE; NONHUMAN; NORMAL HUMAN; PROTEIN ANALYSIS; PROTEIN FOLDING; RENATURATION; SWEETNESS; TOXICITY TESTING;

EID: 0033897334     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0308-8146(00)00151-5     Document Type: Article

References (23)

1. de Vos A.M., Hatada M., van der Wel H., Krabbendam H., Peerdeman A.F., Kim S.H. Three-dimensional structure of thaumatin I, an intensely sweet protein. Proceedings of the National. Acadamy of Sciences. 82:1985;1406-1409.
2. Edens L., Born I., Ledeboer A.M., Maat J., Toonen M.Y., Visser C., Verrips C.T. Synthesis and processing of the plant protein thaumatin in yeast. Cell. 37:1984;629-633.
3. Edens L., Heslinga L., Klok R., Ledeboer A.M., Maat J., Toonen M.Y., Visser C., Verrips C.T. Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli. Gene. 18:1982;1-12.
4. Edens L., Van der Wel H. Microbial synthesis of the sweet-tasting plant protein thaumatin. Trends in Biotechnology. 3:1985;61-64.
5. Etheridge, K. (1994) The sales and marketing of Talin. In M. Witty & J.D. Higginbotham, Thaumatin. Boca Raton, FL:CRC Press.
6. Faus I., del Moral C., Adroer N., del Río J.L., Patiño C., Sisniega H., Casas C., Bladé J., Rubio V. Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori. Applied Microbiology Biotechnology. 49:1998;393-398.
7. Faus I., Patiño C., del Río J.L., del Moral C., Sisniega H., Rubio V. Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli. Biochemical and Biophysical Research Communications. 229:1996;121-127.
8. Hahm Y.T., Batt C.A. Expression and secretion of thaumatin from Aspergillus oryzae. Agric. Biol. Chem. 54:1990;2513-2520.
9. Iyengar R.B., Smits P., Van der Ouderaa F.J.G., Van der Wel H., Van Brouwershaven J.V., Ravestein P., Richters G., Wassenaar P.D. The complete amino acid sequence of the sweet-tasting protein thaumatin I. European Journal of Biochemistry. 96:1979;193-204.
10. Illingworth C., Larson G., Hellekant G. Secretion of the sweet-tasting plant protein thaumatin by Bacillus subtilis. Biotechnology Letters. 10:1988;587-592.
11. Illingworth C., Larson G., Hellekant G. Secretion of the sweet-tasting plant protein thaumatin by Streptomyces lividans. J. Ind. Microbiol. 4:1989;37-42.
12. Kaneko R., Kitabatake N. Heat-induced formation of intermolecular disulfide linkages between thaumatin molecules that do not contain cysteine residues. Journal of Agricultural Food Chemistry. 47:1999;4950-4955.
13. Korver O., Gorkom M.V., Van der Wel H. Spectrometric investigation of thaumatin I and II, two sweet-tasting proteins from Thaumatococcus daniellii Benth. European Journal of Biochemistry. 35:1973;554-558.
14. Moralejo F.J., Cardoza R.E., Gutierrez S., Martin J.F. Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage. Appl. & Environ. Microbiol. 65:1999;1168-1174.
15. Ogata C.M., Gordon P.F., de Vos A.M., Kim S.H. Crystal structure of a sweet tasting protein thaumatin I, at 1 65A resolution. Journal of Molecular Biology. 228:1992;893-908.
16. Provencher S.W., Glöckner J. Estimation of globular protein secondary structure from circular dichroism. Biochemistry. 20:1981;33-37.
17. Perczel A., Hollósi M., Tusnády G., Fasman G.D. Convex constraint analysis. A natural deconvolution of circular dichroism curves of proteins Protein Eng. 4:1991;669-679.
18. Studier F.W. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. Journal of Molecular Biology. 219:1991;37-44.
19. Van der Wel, H. (1994). Structure-activity relations in the thaumatin molecule. In M. Witty, J.D. Higginbotham Thaumatin., Boca Raton, FL:CRC Press.
20. Van der Wel H., Iyengar R.B., Van Brouwershaven J.V., Wassenaar P.D.V., Bel W.J., Van der Ouderaa F.J.G. Assignment of the disulfide bonds in the sweet-tasting protein thaumatin I. European Journal of Biochemistry. 144:1984;41-45.
21. Van der Wel H., Loeve K. Isolation and characterization of thaumatin I and II, the sweet-tasting proteins from Thaumatococcus daniellii Benth. European Journal of Biochemistry. 31:1972;221-225.
22. Witty M. New technologies for taste modifying proteins. Trends in Food Science & Technology. 9:1998;275-280.
23. Zemanek E., Wasserman B.P. Issues and advances in the use of transgenic organisms for the production of thaumatin, the intensely sweet protein from Thaumatococcus daniellii. Crit. Rev. Food Sci. Nutr. 35:1995;455-466.