Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage

Applied and Environmental Microbiology

Volumn 65, Issue 3, 1999, Pages 1168-1174

  Moralejo, Francisco Jose ^a   Cardoza, Rosa Elena ^a   Gutierrez, Santiago ^a,b   Martin, Juan F ^a,b  

^a Institute of Biotechnology INBIOTEC Parque Científico de León   (Spain)

^b UNIVERSITY OF LEÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

THAUMATIN;

ARTICLE; ASPERGILLUS AWAMORI; ASPERGILLUS NIDULANS; CONTROLLED STUDY; GENE CASSETTE; GENE DOSAGE; GROWTH CURVE; NONHUMAN; PROMOTER REGION; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN SECRETION; PROTEIN SYNTHESIS;

ASPERGILLUS AWAMORI; ASPERGILLUS NIDULANS;

EID: 0032985532     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.3.1168-1174.1999     Document Type: Article

References (33)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. Moore, J. A. Smith, J. G. Seidman, and K. Struhl. 1989. Current protocols in molecular biology. Wiley, New York, N.Y.
2. Brakhage, A. A. 1998. Molecular regulation of β-lactam biosynthesis in filamentous fungi. Microbiol. Mol. Biol. Rev. 62:547-585.
3. Brenner, C., and R. S. Fuller. 1992. Structural and enzymatic characterization of a purified prohormone-processing enzyme: secreted, soluble Kex2 protease. Proc. Natl. Acad. Sci. USA 89:922-926.
4. Calmels, T. P. G., F. Martín, H. Durand, and G. Tiraby. 1991. Proteolytic events in the processing of secreted proteins in fungi. J. Biotechnol. 17:51-66.
5. Cardoza, R. E., F. J. Moralejo, S. Gutiérrez, J. Casqueiro, F. Fierro, and J. F. Martín. 1998. Characterization and nitrogen-source regulation at the transcriptional level of the gdhA gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase. Curr. Genet. 34:50-59.
6. Contreras, R., D. Carrez, J. R. Kinghorn, C. A. M. J. J. van den Hondel, and W. Fiers. 1991. Efficient Kex2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Bio/Technology 9:378-381.
7. Dunbar, B. S., and E. D. Schwoebel. 1990. Preparation of polyclonal antibodies. Methods Enzymol. 182:663-670.
8. Edens, L., I. Born, A. M. Ledeboer, J. Maat, M. Y. Toonen, C. Visser, and C. T. Verrips. 1984. Synthesis and processing of the plant protein thaumatin in yeast. Cell 37:629-633.
9. Faus, I., C. del Moral, N. Adroer, J. L. del Río, C. Patińo, H. Sisniega, C. Casas, J. Bladé, and V. Rubio. 1998. Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori. Appl. Microbiol. Biotechnol. 49:393-398.
10. Faus, I., C. Patińo, J. L. del Río, C. del Moral, H. Sisniega, and V. Rubio. 1996. Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli. Biochem. Biophys. Res. Commun. 229:121-127.
11. Fernández, F. J., S. Gutierrez, J. Velasco, E. Montenegro, A. T. Marcos, and J. F. Martín. 1994. Molecular characterization of three loss-of-function mutations in the isopenicillin N-acyltransferase gene (penDE) of Penicillium chrysogenum. J. Bacteriol. 176:4941-4948.
12. Finkelstein, D. B., J. Rambosek, M. S. Crawford, C. L. Soliday, P. C. McAda, and J. Leach. 1989. Protein secretion in Aspergillus niger, p. 295-300. In C. L. Hershberger, S. W. Queener, and G. Hegeman (ed.), Genetics and molecular biology of industrial microorganisms. American Society for Microbiology, Washington, D.C.
13. Fuller, R. S., R. E. Sterne, and J. Thorner. 1988. Enzymes required for yeast prohormone processing. Annu. Rev. Physiol. 50:345-362.
14. Gutiérrez, S., J. Velasco, A. T. Marcos, F. J. Fernández, F. Fierro, J. L. Barredo, B. Díez, and J. F. Martín. 1997. Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum. Appl. Microbiol. Biotechnol. 48:606-614.
15. Gwynne, D. I., and M. Devchand. 1992. Expression of foreign proteins in the genus Aspergillus, p. 203-214. In J. W. Bennet and M. A. Klich (ed.), Aspergillus, the biology and industrial applications. Butterworth, London, United Kingdom.
16. Hahm, Y. T., and C. A. Batt. 1990. Expression and secretion of thaumatin from Aspergillus oryzae. Agric. Biol. Chem. 54:2513-2520.
17. Hammond, C., and A. Helenius. 1995. Quality control in the secretory pathway. Curr. Opin. Cell Biol. 7:523-529.
18. Hijarrubia, M. J., J. Casqueiro, S. Gutiérrez, F. J. Fernández, and J. F. Martin. 1997. Characterization of the hip gene of Aspergillus awamori encoding a protein with an HDEL retention signal homologous to the mammalian BiP involved in polypeptide secretion. Curr. Genet. 32:139-146.
19. Illingworth, C., G. Larson, and G. Hellekant. 1988. Secretion of the sweet-tasting plant protein thaumatin by Bacillus subtilis. Biotechnol. Lett. 10:587-592.
20. Illingworth, C., G. Larson, and G. Hellekant. 1989. Secretion of the sweet-tasting plant protein thaumatin by Streptomyces lividans. J. Ind. Microbiol. 4:37-42.
21. Moralejo, F. J., R. E. Cardoza, S. Gutiérrez, and J. F. Martin. Unpublished data.
22. Punt, P. J., M. A. Dingemanse, J. Kuyvenhoven, R. D. M. Soede, P. H. Pouwels, and C. A. M. J. J. von den Hondel. 1990. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene, encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93:101-109.
23. Punt, P. J., C. Kramer, A. Kuyvenhoven, P. H. Pouwels, and C. A. M. J. J. van den Hondel. 1992. An upstream activating sequence from the Aspergillus nidulans gpdA gene. Gene 120:67-73.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Shen, Y.-Q., J. Heim, N. A. Solomon, S. Wolfe, and A. L. Demain. 1984. Repression of 3-lactam production in Cephalosporium acremonium by nitrogen sources. J. Antibiot. 37:503-511.
26. Van den Homberg, J. P. T. W., P. J. I. van de Vondervoort, L. Fraissinet-Tachet, and J. Visser. 1997. Aspergillus as a host for heterologous protein production: the problem of proteases. Trends Biotechnol. 15:256-263.
27. van den Hondel, C. A. M. J. J., P. J. Punt, and R. F. M. van Gorcom. 1991. Heterologous gene expression in filamentous fungi, p. 396-428. In J. W. Bennett and L. L. Lasure (ed.), More gene manipulation in fungi. Academic Press, San Diego, Calif.
28. Velasco, J., S. Gutiérrez, and J. F. Martín. Unpublished data.
29. Verdoes, J. C., P. J. Punt, and C. A. M. J. J. van den Hondel. 1995. Molecular genetic strain improvement for the over-production of fungal proteins by filamentous fungi. Appl. Microbiol. Biotechnol. 43:195-205.
30. Visser, J., H. J. Bussink, and D. Witteveen. 1995. Gene expression in filamentous fungi, p. 241-308. In A. Smith (ed.), Gene expression in recombinant microorganisms. Marcel Dekker Inc., New York, N.Y.
31. Witty, M., and J. D. Higginbotham. 1994. Thaumatin. CRC Press, Boca Raton, Fla.
32. Yelton, M. M., J. E. Hamer, and W. E. Timberlake. 1984. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc. Natl. Acad. Sci. USA 81:1470-1474.
33. Zemanek, E., and B. P. Wasserman. 1995. Issues and advances in the use of transgenic organisms for the production of thaumatin, the intensely sweet protein from Thaumatococcus daniellii. Crit. Rev. Food Sci. Nutr. 35:455-466.