Spo0A~p imposes a temporal gate for the bimodal expression of competence in bacillus subtilis

PLoS Genetics

Volumn 8, Issue 3, 2012, Pages

  Mirouze, Nicolas ^a   Desai, Yaanik ^b   Raj, Arjun ^b   Dubnau, David ^a,c  

^a PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMK PROTEIN; REGULATOR PROTEIN; REPRESSOR PROTEIN; ROK PROTEIN; SPOOA-P PROTEIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; COMK PROTEIN, BACILLUS SUBTILIS; RHO KINASE; SPO0A PROTEIN, BACILLUS SUBTILIS; TRANSCRIPTION FACTOR;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIUM COMPETENCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; GROWTH CURVE; GROWTH RATE; NONHUMAN; PROMOTER REGION; PROTEIN BINDING; STATIONARY PHASE; BACTERIUM TRANSFORMATION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; DNA TRANSFORMATION COMPETENCE; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; RHO-ASSOCIATED KINASES; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSFORMATION, BACTERIAL;

EID: 84859226953     PISSN: 15537390     EISSN: 15537404     Source Type: Journal    
DOI: 10.1371/journal.pgen.1002586     Document Type: Article

References (43)

1. Losick R, Desplan C, (2008) Stochasticity and cell fate. Science 320: 65-68.
2. Raj A, van Oudenaarden A, (2008) Nature, nurture, or chance: stochastic gene expression and its consequences. Cell 135: 216-226.
3. Balazsi G, van Oudenaarden A, Collins JJ, (2011) Cellular decision making and biological noise: from microbes to mammals. Cell 144: 910-925.
4. Cozy LM, Kearns DB, (2010) Gene position in a long operon governs motility development in Bacillus subtilis. Mol Microbiol 76: 273-285.
5. Suel GM, Garcia-Ojalvo J, Liberman LM, Elowitz MB, (2006) An excitable gene regulatory circuit induces transient cellular differentiation. Nature 440: 545-550.
6. Suel GM, Kulkarni RP, Dworkin J, Garcia-Ojalvo J, Elowitz MB, (2007) Tunability and noise dependence in differentiation dynamics. Science 315: 1716-1719.
7. Cagatay T, Turcotte M, Elowitz MB, Garcia-Ojalvo J, Suel GM, (2009) Architecture-dependent noise discriminates functionally analogous differentiation circuits. Cell 139: 512-522.
8. Maamar H, Raj A, Dubnau D, (2007) Noise in gene expression determines cell fate in Bacillus subtilis. Science 317: 526-529.
9. Veening JW, Smits WK, Hamoen LW, Kuipers OP, (2006) Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media. J Appl Microbiol 101: 531-541.
10. Veening JW, Igoshin OA, Eijlander RT, Nijland R, Hamoen LW, et al. (2008) Transient heterogeneity in extracellular protease production by Bacillus subtilis. Mol Syst Biol 4: 184.
11. Leisner M, Stingl K, Radler JO, Maier B, (2007) Basal expression rate of comK sets a 'switching-window' into the K-state of Bacillus subtilis. Mol Microbiol 63: 1806-1816.
12. Errington J, (2003) Regulation of endospore formation in Bacillus subtilis. Nat Rev Microbiol 1: 117-126.
13. Dubnau D, Lovett CM Jr, (2002) Transformation and Recombination. In: Hoch JA, Losick R, Sonenshein AL, editors. Bacillus subtilis and Its Relatives: From Genes to Cells Washington, DC American Society for Microbiology pp. 453-471.
14. Lopez D, Vlamakis H, Kolter R, (2010) Biofilms. Cold Spring Harb Perspect Biol 2: a000398.
15. Chai Y, Chu F, Kolter R, Losick R, (2008) Bistability and biofilm formation in Bacillus subtilis. Mol Microbiol 67: 254-263.
16. Haseltine-Cahn F, Fox MS, (1968) Fractionation of transformable bacteria from competent cultures of Bacillus subtilis on renografin gradients. J Bacteriol 95: 867-875.
17. Hadden C, Nester EW, (1968) Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol 95: 876-885.
18. van Sinderen D, Venema G, (1994) comK acts as an autoregulatory control switch in the signal transduction route to competence in Bacillus subtilis. J Bacteriol 176: 5762-5770.
19. Smits WK, Eschevins CC, Susanna KA, Bron S, Kuipers OP, et al. (2005) Stripping Bacillus: ComK auto-stimulation is responsible for the bistable response in competence development. Mol Microbiol 56: 604-614.
20. Maamar H, Dubnau D, (2005) Bistability in the Bacillus subtilis K-state (competence) system requires a positive feedback loop. Mol Microbiol 56: 615-624.
21. Leisner M, Stingl K, Frey E, Maier B, (2008) Stochastic switching to competence. Curr Opin Microbiol 11: 553-559.
22. Leisner M, Kuhr J-T, Radler JO, Frey E, Maier B, (2009) Kinetics of genetic switching into the state of bacterial competence. Biophys J 96: 1178-1188.
23. Mirouze N, Prepiak P, Dubnau D, (2011) Fluctuations in spo0A transcription control rare developmental transitions in Bacillus subtilis. PLoS Genet 7: e1002048.
24. Albano M, Hahn J, Dubnau D, (1987) Expression of competence genes in Bacillus subtilis. J Bacteriol 169: 3110-3117.
25. Burbulys D, Trach KA, Hoch JA, (1991) Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell 64: 545-552.
26. Hoa TT, Tortosa P, Albano M, Dubnau D, (2002) Rok (YkuW) regulates genetic competence in Bacillus subtilis by directly repressing comK. Mol Microbiol 43: 15-26.
27. Hamoen LW, Van Werkhoven AF, Bijlsma JJE, Dubnau D, Venema G, (1998) The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix. Genes Dev 12: 1539-1550.
28. Smits WK, Hoa TT, Hamoen LW, Kuipers OP, Dubnau D, (2007) Antirepression as a second mechanism of transcriptional activation by a minor groove binding protein. Mol Microbiol 64: 368-381.
29. Smits WK, Grossman AD, (2010) The transcriptional regulator Rok binds A+T-rich DNA and is involved in repression of a mobile genetic element in Bacillus subtilis. PLoS Genet 6: e1001207.
30. Molle V, Fujita M, Jensen ST, Eichenberger P, Gonzalez-Pastor JE, et al. (2003) The Spo0A regulon of Bacillus subtilis. Mol Microbiol 50: 1683-1701.
31. Chai Y, Norman T, Kolter R, Losick R, (2011) Evidence that metabolism and chromosome copy number control mutually exclusive cell fates in Bacillus subtilis. Embo J 30: 1402-1413.
32. Serror P, Sonenshein AL, (1996) CodY is required for nutritional repression of Bacillus subtilis genetic competence. J Bacteriol 178: 5910-5915.
33. Haijema BJ, Hahn J, Haynes J, Dubnau D, (2001) A ComGA-dependent checkpoint limits growth during the escape from competence. Mol Microbiol 40: 52-64.
34. Gonzalez-Pastor JE, Hobbs EC, Losick R, (2003) Cannibalism by sporulating bacteria. Science 301: 510-513.
35. Fujita M, Gonzalez-Pastor JE, Losick R, (2005) High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis. J Bacteriol 187: 1357-1368.
36. Elowitz MB, Levine AJ, Siggia ED, Swain PS, (2002) Stochastic gene expression in a single cell. Science 297: 1183-1186.
37. Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329: 533-538.
38. Chastanet A, Vitkup D, Yuan GC, Norman TM, Liu JS, et al. (2010) Broadly heterogeneous activation of the master regulator for sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A 107: 8486-8491.
39. Gillespie D, (1977) Exact stochastic simulation of coupled chemical reactions. J Phys Chem 81: 2340-2361.
40. Banse AV, Chastanet A, Rahn-Lee L, Hobbs EC, Losick R, (2008) Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis. Proc Natl Acad Sci U S A 105: 15547-15552.
41. Muchova K, Lewis RJ, Perecko D, Brannigan JA, Ladds JC, et al. (2004) Dimer-induced signal propagation in Spo0A. Mol Microbiol 53: 829-842.
42. Prepiak P, Defrancesco M, Spadavecchia S, Mirouze N, Albano M, et al. (2011) MecA dampens transitions to spore, biofilm exopolysaccharide and competence expression by two different mechanisms. Mol Microbiol 80: 1014-1030.
43. Buckler DR, Stock AM, (2000) Synthesis of [(32)P]phosphoramidate for use as a low molecular weight phosphodonor reagent. Anal Biochem 283: 222-227.