A neurosteroid analogue photolabeling reagent labels the colchicine-binding site on tubulin: A mass spectrometric analysis

Electrophoresis

Volumn 33, Issue 4, 2012, Pages 666-674

  Chen, Zi Wei ^a   Chen, Li Hai ^a,b   Akentieva, Natalia ^a   Lichti, Cheryl F ^a   Darbandi, Ramin ^a   Hastings, Randy ^a   Covey, Douglas F ^a   Reichert, David E ^c   Townsend, R Reid ^a   Evers, Alex S ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^c WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Mass spectrometry; Neurosteroids; Photolabeling; Tubulin

Indexed keywords

AMINO ACIDS; DESORPTION; ELECTROPHORESIS; LIQUID CHROMATOGRAPHY; MAMMALS; PROTEINS; PURIFICATION;

BINDING-SITES; BOVINE BRAIN; COLCHICINE BINDING; MASS SPECTROMETRIC ANALYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION; MATRIXASSISTED LASER DESORPTION-IONIZATION (MALDI); NEUROSTEROIDS; PHOTOLABELING; PHOTOLABELS; TUBULIN;

MASS SPECTROMETRY;

2 METHOXYESTRADIOL; 6 AZIPREGNANOLONE; ALPHA TUBULIN; BETA TUBULIN; BETA TUBULIN ANTIBODY; BRAIN PROTEIN; COLCHICINE; CYSTEINE; ESTRADIOL; ISOPROTEIN; NEUROSTEROID; PEPTIDE; PROTEIN ANTIBODY; REAGENT; THREONYLALANYLVALYLCYSTEINYLASPARTYLISOLEUCYLPROLYLPROLYLARGININE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; COVALENT BOND; DRUG BINDING SITE; DRUG PROTEIN BINDING; ELECTROSPRAY MASS SPECTROMETRY; HORMONE STRUCTURE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; ISOELECTRIC POINT; LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NANOANALYSIS; NONHUMAN; PHOTOAFFINITY LABELING; PREDICTION; PROTEIN DETERMINATION; PROTEIN PURIFICATION; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 84858983826     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201100434     Document Type: Article

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