Sample preparation and in-solution protease digestion of proteins for chromatography-based proteomic analysis

Current Protocols in Protein Science

Volumn , Issue SUPPL.53, 2008, Pages

  Washburn, Michael P ^a  

^a STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

Author keywords

MudPIT; Multidimensional protein identification technology; Protein; Proteolysis in solution; Proteomics; Trypsin digestion

Indexed keywords

PROTEINASE; PROTEIN; PROTEOME; ARGININE; ASPARAGINE; CATION EXCHANGE RESIN; CHYMOTRYPSIN; ELASTASE; LYSINE DECARBOXYLASE; PROPROTEIN CONVERTASE 9; PROTEINASE K; TRICHLOROACETIC ACID; TRYPSIN;

ANALYTIC METHOD; CHROMATOGRAPHY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME SPECIFICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MULTIDIMENTIONAL PROTEIN IDENTIFICATION TECHNOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEOMICS; REVIEW; SEPARATION TECHNIQUE; TANDEM MASS SPECTROMETRY; ARTICLE; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; CATION EXCHANGE; CHROMATOGRAPHY BASED PROTEOMICS; COMPLEX FORMATION; ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; HYDROPHILICITY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MULTIDIMENSIONAL PROTEIN IDENTIFICATION TECHNOLOGY; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS; VISCOSITY;

CHROMATOGRAPHY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENDOPEPTIDASES; HUMANS; PROTEINS; PROTEOME; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 55249104730     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2306s53     Document Type: Review

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