Pancreatic islets from dexamethasone-treated rats show alterations in global gene expression and mitochondrial pathways

General Physiology and Biophysics

Volumn 31, Issue 1, 2012, Pages 65-76

  Roma, Letícia P ^a,b   Souza, Kleber L A ^a   Carneiro, Everardo M ^a   Boschero, Antonio C ^a   Bosqueiro, José R ^c  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Cell viability; Diabetes; Gene expression; Insulin secretion

Indexed keywords

COMPLEMENTARY DNA; DEXAMETHASONE; DEXANIL; FATTY ACID; GLUCOCORTICOID; GLUCOSE; INSULIN; MESSENGER RNA; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL TYPE; CELL VIABILITY; CONTROLLED STUDY; CORTICOSTEROID THERAPY; DRUG MECHANISM; FATTY ACID BLOOD LEVEL; GENE EXPRESSION REGULATION; GENE SEQUENCE; IMMUNOBLOTTING; INSULIN RELEASE; MALE; MITOCHONDRION; NON INSULIN DEPENDENT DIABETES MELLITUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PANCREAS ISLET; POLYMERASE CHAIN REACTION; RADIOIMMUNOASSAY; RAT; TARGET CELL; TREATMENT OUTCOME; TRIACYLGLYCEROL BLOOD LEVEL; UNINDEXED SEQUENCE;

ANIMALS; CELLS, CULTURED; DEXAMETHASONE; GENE EXPRESSION REGULATION; GLUCOCORTICOIDS; ISLETS OF LANGERHANS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; RATS; RATS, WISTAR; REACTIVE OXYGEN SPECIES; SIGNAL TRANSDUCTION;

MAMMALIA; RATTUS;

EID: 84858763067     PISSN: 02315882     EISSN: 13384325     Source Type: Journal    
DOI: 10.4149/gpb_2012_011     Document Type: Article

References (41)

1. Adams J. M., Cory S. (2001): Life-or-death decisions by the Bcl-2 protein family. Trends Biochem. Sci. 26, 61-66 http://dx.doi.org/10.1016/S0968-0004(00) 01740-0 (Pubitemid 32124712)
2. Auphan N., DiDonato J. A., Rosette C., Helmberg A., Karin M. (1995): Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis. Science 270, 286-290 http://dx.doi.org/10. 1126/science.270.5234.286
3. Barseghian G., Levine R. (1980): Effect of corticosterone on insulin and glucagon secretion by the isolated perfused rat pancreas. Endocrinology 106, 547-552 http://dx.doi.org/10.1210/endo-106-2-547 (Pubitemid 10164172)
4. Beato M. (1991): Transcriptional control by nuclear receptors. FASEB. J. 5, 2044-2051 (Pubitemid 121000168)
5. Beato M., Herrlich P., Schutz G. (1995): Steroid hormone receptors: many actors in search of a plot. Cell 83, 851-857 http://dx.doi.org/10.1016/0092- 8674(95)90201-5 (Pubitemid 26006527)
6. Brunstedt J., Nielsen J. H. (1981): Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta Endocrinol. (Copenh.) 96, 498-504 (Pubitemid 11110431)
7. Chrysis D., Zaman F., Chagin A. S., Takigawa M., Savendahl L. (2005): Dexamethasone induces apoptosis in proliferative chondrocytes through activation of caspases and suppression of the Akt-phosphatidylinositol 3'-kinase signaling pathway. Endocrinology 146,1391-1397 http://dx.doi.org/10.1210/en.2004-1152 (Pubitemid 40289327)
8. Cole T. G., Wilcox H. G., Heimberg, M. (1982): Effects of adrenalectomy and dexamethasone on hepatic lipid metabolism. J. Lipid. Res. 23, 81-91 (Pubitemid 12112615)
9. Cory S., Adams J. M. (2002): The Bcl2 family: regulators of the cellular life-or-death switch. Nat. Rev. Cancer 2, 647-656 http://dx.doi.org/10.1038/ nrc883 (Pubitemid 37328916)
10. Delaunay F., A. Khan, Cintra A., Davani B., Ling Z. C., Andersson A., Ostenson C. G., Gustafsson J., Efendic S., Okret S. (1997): Pancreatic beta cells are important targets for the diabetogenic effects of glucocorticoids. J. Clin. Invest. 100, 2094-2098 http://dx.doi.org/10.1172/JCI119743 (Pubitemid 27459365)
11. Eizirik D. L., Mandrup-Poulsen T. (2001): A choice of death-the signal-transduction of immune-mediated beta-cell apoptosis. Diabetologia 44, 2115-2133 http://dx.doi.org/10.1007/s001250100021 (Pubitemid 34015464)
12. El-Assaad W., Joly E., Barbeau A., Sladek R., Buteau J., Maestre I., Pepin E., Zhao S., Iglesias J., Roche E., Prentki M. (2010): Glucolipotoxicity alters lipid partitioning and causes mitochondrial dysfunction, cholesterol, and ceramide deposition and reactive oxygen species production in INS832/13 ss-cells. Endocrinology 151, 3061-3073 http://dx.doi.org/10.1210/en.2009-1238
13. Fischer B., Rausch U., Wollny P., Westphal H., Seitz J., Aumuller G. (1990): Immunohistochemical localization of the glucocorticoid receptor in pancreatic beta-cells of the rat. Endocrinology 126, 2635-2641 http://dx.doi.org/10.1210/endo-126-5-2635 (Pubitemid 20173057)
14. Hoogwerf B., Danese R. D. (1999): Drug selection and the management of corticosteroid-related diabetes mellitus. Rheum. Dis. Clin. North. Am. 25, 489-505 http://dx.doi.org/10.1016/S0889-857X(05)70083-1 (Pubitemid 29400386)
15. Jeong I. K., Oh S. H., Kim B. J., Chung J. H., Min Y. K., Lee M. S., Lee M. K., Kim K. W. (2001): The effects of dexamethasone on insulin release and biosynthesis are dependent on the dose and duration of treatment. Diabetes Res. Clin. Pract. 51, 163-171 http://dx.doi.org/10.1016/S0168-8227(00)00229-1
16. Jorns A., Sennholz C., Naujok O., Lenzen S. (2010): Beta cell mass regulation in the rat pancreas through glucocorticoids and thyroid hormones. Pancreas 39, 1167-1172 http://dx.doi.org/10.1097/MPA.0b013e3181dfce4f
17. Kaneto H., Matsuoka T. A., Nakatani Y., Kawamori D., Matsuhisa M., Yamasaki Y. (2005): Oxidative stress and the JNK pathway in diabetes. Curr. Diabetes Rev. 1, 65-72 http://dx.doi.org/10.2174/1573399052952613
18. Kassel O., Sancono A., Kratzschmar J., Kreft B., Stassen M., Cato A. C. (2001): Glucocorticoids inhibit MAP kinase via increased expression and decreased degradation of MKP-1. EMBO J. 20, 7108-7116 http://dx.doi.org/10.1093/ emboj/20.24.7108 (Pubitemid 34062302)
19. Kaufmann S. H., Hengartner M. O. (2001): Programmed cell death: alive and well in the new millennium. Trends Cell. Biol. 11, 526-534 http://dx.doi.org/ 10.1016/S0962-8924(01)02173-0 (Pubitemid 33079147)
20. Kawai A., Kuzuya N. (1977): On the role of glucocorticoid in glucose-induced insulin secretion. Horm. Metab. Res. 9, 361-365 http://dx.doi.org/10.1055/s-0028-1093528 (Pubitemid 8196613)
21. Kutlu B., Cardozo A. K., Darville M., I.Kruhoffer M., Magnusson N., Orntoft T., Eizirik D. L. (2003): Discovery of gene networks regulating cytokine-induced dysfunction and apoptosis in insulin-producing INS-1 cells. Diabetes 52, 2701-2719 http://dx.doi.org/10.2337/diabetes.52.11.2701 (Pubitemid 37339700)
22. Lambillotte C., Gilon P., Henquin J. C. (1997): Direct glucocorticoid inhibition of insulin secretion. An in vitro study of dexamethasone effects in mouse islets. J. Clin. Invest. 99, 414-423 http://dx.doi.org/10.1172/JCI119175 (Pubitemid 27414798)
23. Lenzen S., Bailey C. J. (1984): Thyroid hormones, gonadal and adrenocortical steroids and the function of the islets of Langerhans. Endocr. Rev. 5, 411-434 http://dx.doi.org/10.1210/edrv-5-3-411
24. Mizuno N., Yoshitomi H., Ishida H., Kuromi H., Kawaki J., Seino Y., Seino S. (1998): Altered bcl-2 and bax expression and intracellular Ca2+ signaling in apoptosis of pancreatic cells and the impairment of glucose-induced insulin secretion. Endocrinology 139, 1429-1439 http://dx.doi.org/10.1210/en.139.3.1429 (Pubitemid 28510165)
25. Nicod N., Giusti V., Besse C., Tappy L. (2003): Metabolic adaptations to dexamethasone-induced insulin resistance in healthy volunteers. Obes. Res. 11, 625-631 http://dx.doi.org/10.1038/oby.2003.90 (Pubitemid 41151613)
26. Oltvai Z. N., Milliman C. L., Korsmeyer S. J. (1993): Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74, 609-619 http://dx.doi.org/10.1016/0092-8674(93) 90509-O (Pubitemid 23259742)
27. Patel J. V., Cummings D. E., Girod J. P., Mascarenhas A.V., Hughes E. A., Gupta M., Lip G. Y., Reddy S., Brotman D. J. (2006): Role of metabolically active hormones in the insulin resistance associated with short-term glucocorticoid treatment. J. Negat. Results Biomed. 5, 14 http://dx.doi.org/10. 1186/1477-5751-5-14
28. Pick A., Clark J., Kubstrup C., Levisetti M., Pugh W., Bonner-Weir S., Polonsky K. S. (1998): Role of apoptosis in failure of beta-cell mass compensation for insulin resistance and beta-cell defects in the male Zucker diabetic fatty rat. Diabetes 47, 358-364 http://dx.doi.org/10.2337/diabetes.47. 3.358 (Pubitemid 28104196)
29. Pierluissi J., Navas F. O., Ashcroft S. J. (1986): Effect of adrenal steroids on insulin release from cultured rat islets of Langerhans. Diabetologia 29, 119-121 http://dx.doi.org/10.1007/BF00456122 (Pubitemid 16162755)
30. Porte D. Jr., Kahn S. E. (2001): beta-cell dysfunction and failure in type 2 diabetes: potential mechanisms. Diabetes 50 (Suppl. 1), S160-163 http://dx.doi.org/10.2337/diabetes.50.2007.S160 (Pubitemid 32148244)
31. Rafacho A., Roma L. P., Taboga S. R., Boschero A. C., Bosqueiro J. R. (2007): Dexamethasone-induced insulin resistance is associated with increased connexin 36 mRNA and protein expression in pancreatic rat islets. Can. J. Physiol. Pharmacol. 85, 536-545 http://dx.doi.org/10.1139/Y07-037 (Pubitemid 47309800)
32. Rafacho A., Cestari T. M., Taboga S. R., Boschero A. C., Bosqueiro J. R. (2009): High doses of dexamethasone induce increased beta-cell proliferation in pancreatic rat islets. Am. J. Physiol. Endocrinol. Metab. 296, E681-689 http://dx.doi.org/10.1152/ajpendo.90931.2008
33. Ranta F., Avram D., Berchtold S., Dufer M., Drews G., Lang F., Ullrich S. (2006): Dexamethasone induces cell death in insulin- secreting cells, an effect reversed by exendin-4. Diabetes 55, 1380-1390 http://dx.doi.org/10.2337/db05- 1220 (Pubitemid 44195377)
34. Roma L. P., Bosqueiro J. R., Cunha D. A., Carneiro E. M., Gurgul-Convey E., Lenzen S., Boschero A. C., Souza K. L. (2009): Protection of insulin-producing cells against toxicity of dexamethasone by catalase overexpression. Free Radic. Biol. Med. 47, 1386-1393 http://dx.doi.org/10.1016/ j.freeradbiomed.2009.08.010
35. Roma L. P., Oliveira C. A., Carneiro E. M., Albuquerque G. G., Boschero A. C., Souza K. L. (2011): N-acetylcysteine protects pancreatic islet against glucocorticoid toxicity. Redox. Rep. 16, 173-180 http://dx.doi.org/10.1179/ 1351000211Y.0000000006
36. Schaaf M. J., Cidlowski J. A. (2002): Molecular mechanisms of glucocorticoid action and resistance. J. Steroid Biochem. Mol. Biol. 83, 37-48 http://dx.doi.org/10.1016/S0960-0760(02)00263-7 (Pubitemid 37040640)
37. Schafroth U., Godang K., Ueland T., Berg J. P., Bollerslev J. (2000): Leptin levels in relation to body composition and insulin concentration in patients with endogenous Cushing's syndrome compared to controls matched for body mass index. Endocrinol. Invest. 23, 349-355 (Pubitemid 30433173)
38. Schmidt S., Rainer J., Ploner C., Presul E., Riml S., Kofler R. (2004): Glucocorticoid-induced apoptosis and glucocorticoid resistance: molecular mechanisms and clinical relevance. Cell Death Differ. 11 (Suppl. 1), S45-55 http://dx.doi.org/10.1038/sj.cdd.4401456 (Pubitemid 39136798)
39. Souza K. L., Elsner M., Mathias P. C., Lenzen S., Tiedge M. (2004): Cytokines activate genes of the endocytotic pathway in insulin-producing RINm5F cells. Diabetologia 47, 1292-1302 http://dx.doi.org/10.1007/s00125-004-1435-2 (Pubitemid 39093326)
40. Weinhaus A. J., Bhagroo N. V., Brelje T. C., Sorenson R. L. (2000): Dexamethasone counteracts the effect of prolactin on islet function: implications for islet regulation in late pregnancy. Endocrinology 141, 1384-1393 http://dx.doi.org/10.1210/en.141.4.1384 (Pubitemid 32269106)
41. Zhang S., Liu J., Saafi E. L., Cooper G. J. (1999): Induction of apoptosis by human amylin in RINm5F islet beta-cells is associated with enhanced expression of p53 and p21WAF1/CIP1. FEBS Lett. 455, 315-320 http://dx.doi.org/10.1016/S0014-5793(99)00894-7 (Pubitemid 29333059)